Abstract: Objective To practice the tutorial system in the general practitioners transfer training and discuss the training effect. Methods A total of 295 general practitioners who were trained in the First Affiliated Hospital of Chongqing Medical University(Chongqing general practice medical education center) from 2015 to 2017 were selected as the objects of the study, and the tutorial system was adopted for training. Before the training, the entrance examination were carried out. After the training, the Chongqing Municipal Health Committee uniformly organized the completion theory examination, cardiopulmonary resuscitation(CPR) and clinical practice skill examination of the same difficulty, which were evaluated by the percentage system, and 60 points were considered as passing. Results Before training, the failure rate of CPR and clinical practice skills was nearly 60%(59.36% vs. 57.24%), and the failure rate after training were significantly decreased(1.41% vs. 0.71%), After cardiopulmonary resuscitation and clinical practice skill training, female doctors were better than male doctors, with statistically significant difference(all P<0.05). There was no gender difference in the theoretical examination at the end of the course(P>0.05). The score of technical secondary school graduates before clinical practice skill training was significantly lower than that of college graduates and undergraduate graduates, and the increase of score after training was the highest(all P<0.05). Working years were negatively correlated with the score of CPR and clinical practice skill after training(CPR: r=-0.158, P=0.007; Clinical practice skills: r=-0.121, P=0.004). There was no correlation between education level and CPR and clinical practice skills after training(CPR: r=0.101, P=0.083; Clinical practice skills: r=0.062, P=0.293), but there was a positively correlated with theoretical test scores(r=0.282, P<0.001). Conclusion Through the transfer training program of general practitioners under the tutorial system, the trainees have significantly improved their skills in CPR and clinical practice, and passed the theoretical examination. It is suggested that the training program of general practitioner transfer under the tutorial system can effectively improve the diagnosis and treatment ability of general practitioners.