Abstract: Objective To investigate the application value of gamma globulin in the treatment of sepsis. Methods A total of 118 cases of sepsis patients in our hospital were randomly divided into two groups, 59 cases each group. The control group was received basic treatment, and the observation group was treated with gamma globulin on the basis of the control group. The changes in clinical indicators(SOFA score, APACHE Ⅱ score, WBC, HR), T lymphocyte subsets(CD4⁺, CD8⁺, CD4⁺/CD8⁺), immunoglobulin(IgG, IgM, IgA) and serum inflammatory factors(CRP, TNF-α, IL-10) were compared between the two groups before and after treatment. Results Compared with the control group after treatment, the SOFA score(6.72±1.25), APACHE Ⅱ score(11.19±2.09), WBC(7.82±1.32) and HR(83.29±15.35) in the observation group were significantly reduced(all P<0.001). After treatment, the level of T lymphocyte subsets(CD4⁺, CD8⁺ and CD4⁺/CD8⁺) in the observation group was significantly improved(all P<0.05), and the improvement effect was significantly better than that in the control group(all P<0.05). Meanwhile, in the control group, CD4⁺ and CD4⁺/CD8⁺ were significantly improved(all P<0.05). IgG levels in the observation group were significantly increased(P<0.05) and significantly higher than those in the control group(P<0.05). There was no significant change in humoral immunity in the control group(all P>0.05). After treatment, the levels of pro-inflammatory factors(CRP, TNF-α) in the two groups were significantly decreased, and those in the observation group were lower than those in the control group(P<0.05). The level of anti-inflammatory factor IL-10 in the two groups significantly increased(all P<0.05), and that in the observation group was significantly higher than that in the control group(all P<0.05). Conclusion Gamma globulin adjuvant therapy can significantly enhance the cellular immunity and humoral immunity of patients with sepsis, improve the level of serum inflammatory factors, and contribute to the improvement of patients' condition.