Abstract: Objective To investigate the effect of autogenous dentin particles combining platelet rich plasma in treatment of periodontal bone defect. Methods A total of 84 cases of single tooth loss and horizontal bone defect in the anterior maxilla require single implantation and guided bone regeneration patients in our hospital from October 2016 to February 2018 were divided into research group(42 cases) and control group(42 cases). The patients in research group received autogenous dentin particles combining platelet rich plasma, while the patients in control group received connective tissue graft re-establish treatment. All patients were tested by the Cone Bean Computed Tomographies(CBCT) at following time: T0(before surgery), T1(2 weeks after surgery), and T2(1 year after surgery). The buccal soft tissue profile(BSP), buccal bone thickness(BB), buccal soft tissue thickness(BST), repair success rate, complication rate, and pink esthetic score(PES) of the two groups were compared. Results There were statistically significant differences in BSP, BB, and BST between the two groups and time points(all P<0.05). The BB of the control group was larger than that of the research group at T1(P<0.05). The BSP of the research group at T2 was larger than that of the control group(P<0.05), and there was no significant difference in BB, BST between two groups(all P>0.05). There was no significant difference in repair success rate, complication rate and PES between the two groups(all P>0.05). Conclusion Autogenous dentin particles combined with platelet rich plasma have significant efficacy in guiding bone regeneration for the treatment of periodontal bone defects, doing well in aesthetic requirements of single anterior implants.