Changes of aromatic hydrocarbon receptor and cyclooxygenase-2 levels in bronchial lavage fluid of patients with chronic obstructive pulmonary disease and its clinical significance
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摘要: 目的 探讨慢性阻塞性肺疾病患者支气管灌洗液中芳香烃受体(AHR)和环氧合酶-2(COX-2)水平变化及临床意义。 方法 选取2011年10月—2013年10月我院呼吸内科确诊的COPD患者94例为病例组,健康体检者88例作为对照组,测定支气管灌洗液中AHR、COX-2、CD44、Th17、IL-4、IL-8、IL-10、TNF-α、hs-CRP水平。采用Pearson相关分析及Logistic回归分析探讨AHR及COX-2对COPD患者的诊断及预后意义。 结果 病例组支气管灌洗液中AHR、IFN-γ水平低于对照组,COX-2、CD44、IL-4、IL-8、IL-10、TNF-α、hs-CRP、TH17水平高于对照组,差异有统计学意义(均P<0.05);AHR与CD44、IL-4、IL-8、IL-10、TNF-α、hs-CRP、TH17负相关关系明显,与IFN-γ呈正相关关系(均P<0.05);COX-2与CD44、IL-4、IL-8、IL-10、TNF-α、hs-CRP、TH17正相关关系,与IFN-γ呈负相关关系(均P<0.05);高水平的COX-2及低水平的AHR均为慢性阻塞性肺疾病患者出现死亡的危险因素(均P<0.05);COX-2、AHR两者的AUC相近,皆小于AHR+COX-2;COX-2、AHR诊断慢性阻塞性肺疾病的灵敏度、特异度相近(均P>0.05),皆小于AHR+COX-2(均P<0.05)。 结论 慢性阻塞性肺疾病患者支气管灌洗液中芳香烃受体水平降低,环氧合酶-2水平升高,其引发一系列炎症因子失衡可能是慢性阻塞性肺疾病的发病机制;AHR+COX-2诊断慢性阻塞性肺疾病具有较高的灵敏度、特异度,值得在临床上推广应用。Abstract: Objective To investigate the changes of aromatic hydrocarbon receptor and cyclooxygenase-2 levels in bronchial lavage fluid of patients with chronic obstructive pulmonary disease and its clinical significance. Methods Ninety-four COPD patients diagnosed in respiratory department of our hospital were selected as case group and 88 healthy persons as control group. The levels of aromatic hydrocarbon receptor(AHR), cyclooxygenase-2(COX-2), CD44, Th17, IL-4, IL-8, IL-10, TNF-α and hs-CRP in bronchial lavage fluid were measured. Pearson correlation analysis and logistic regression analysis were used to explore the diagnostic and prognostic significance of AHR and COX-2 in COPD patients. Results The levels of AHR and IFN-γ in bronchial lavage fluid of the case group were lower than those of the control group, and the levels of COX-2, CD44, IL-4, IL-8, IL-10, TNF-α, hs-CRP and TH17 were higher than those of the control group(all P<0.05). The negative correlation between AHR and CD44, IL-4, IL-8, IL-10, TNF-α, hs-CRP, TH17 were significant, and the positive correlation between COX-2 and CD44, IL-4, IL-8, IL-10 were significant(all P<0.05). The TNF-α, hs-CRP and TH17 were positively correlated with IFN-γ(all P<0.05). High levels of COX-2, TH17 and low level of AHR were risk factors for death in patients with COPD(P<0.05). The AUC of COX-2 and AHR were similar, both were less than that of AHR + COX-2. The sensitivity and specificity of COX-2 and AHR in diagnosis of COPD were similar(P>0.05). They were all less than that of AHR + COX-2(P<0.05). Conclusion The level of AHR in bronchial lavage fluid of COPD patients decreased, and the level of COX-2 increased. The imbalance of a series of inflammatory factors may be the pathogenesis of COPD. AHR + COX-2 has high sensitivity and specificity in the diagnosis of COPD, and is worthy of clinical application.
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