Expression of miR-155 in villus tissues and its relationship with TIM3 level in patients with recurrent abortion
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摘要: 目的 探讨复发性流产患者绒毛组织中miR-155表达水平及其与TIM3水平的关系。 方法 选择2016年8月—2018年3月在杭州市妇产科医院行清宫术的63例复发性流产患者为研究组,同期行清宫术的57例正常早孕终止妊娠者为对照组。采集2组受试者绒毛组织,检测miR-155、TIM3、INF-γ、IL-4和IL-13表达水平,并分析研究组miR-155与TIM3、INF-γ、IL-4和IL-13的关系。 结果 研究组miR-155、IL-4和IL-13 mRNA表达水平低于对照组(均P<0.05),TIM3、INF-γ、IL-2 mRNA表达水平高于对照组(均P<0.05);研究组TIM3蛋白表达水平高于对照组(P<0.05);研究组miR-155与TIM3、INF-γ、IL-2呈负相关(r=-0.687、-0.636、-0.602,均P<0.05),与IL-4、IL-13呈正相关(r=0.574、0.498,均P<0.05);TIM3与INF-γ、IL-2呈正相关(r=0.713、0.644,均P<0.05),与IL-4、IL-13呈负相关(r=-0.615、-0.597,均P<0.05)。 结论 miR-155在复发性流产患者绒毛组织中表达明显降低,与TIM3存在明显负相关,两者可能在介导复发性流产过程中存在相互作用。
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关键词:
- 复发性流产 /
- 绒毛组织 /
- miR-155 /
- T细胞免疫球蛋白黏蛋白分子-3
Abstract: Objective To investigate the expression of miR-155 in villus tissues of recurrent abortion patients and its relationship with TIM3 level. Methods From August 2016 to March 2018, 63 cases of recurrent abortion patients underwent complete curettage of uterine cavity in Gynecology and Obstetrics Clinic of Hangzhou gynecology and obstetrics hospital were selected as the study group, at the same time, 57 cases of normal early pregnancy women who terminate the pregnancy by complete curettage of uterine cavity were selected as control group. The villus tissues of the two groups were collected to detect the expression levels of miR-155, TIM3, INF-γ, IL-4 and IL-13, and the relationship between miR-155 and TIM3, INF-γ, IL-4 and IL-13 of the study group were analyzed. Results The expression levels of miR-155, IL-4 and IL-13 mRNA in the study group were lower than those in the control group(all P<0.05), and the expression levels of TIM3, INF-γ and IL-2 mRNA were higher than those in the control group(all P<0.05). The expression level of TIM3 protein was higher than that of the control group(P<0.05). The miR155 of the study group was negatively correlated with TIM3, INF-γ and IL-2(r=-0.687,-0.636,-0.602, P<0.05), and positively correlated with IL-4 and IL-13(r=0.574, 0.498, P<0.05). TIM3 was positively correlated with INF-γ and IL-2, respectively(r=0.713, 0.644, P<0.05), and negatively correlated with IL-4 and IL-13(r=-0.615,-0.597, P<0.05). Conclusion The expression of miR-155 in villus tissues of patients with recurrent spontaneous abortion is decreased significantly, it is significantly negatively correlated with TIM3, they may interact with each other in the process of recurrent spontaneous abortion.-
Key words:
- Recurrent abortion /
- Villous tissue /
- MiR-155 /
- T-cell immunoglobulin-mucin molecule-3
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