Abstract: Objective To investigate the effect of Huayu Sanjie therapy on the expression of progesterone receptor(PR), proliferating cell nuclear associated antigen Ki-67(Ki-67), B-cell lymphoma/leukemia-2 gene(Bcl-2) in endometrium of patients after transcervical resection of polyp(TCRP). Methods A total of 216 patients admitted by Ningbo Hospital of Traditional Chinese medicine from May 2016 to May 2018 were divided into A, B and C groups according to the random number method, with 72 case each group. Group A was given Huayu Sanjie prescription oral plus external application, group B was given marvelon, group C was not given any drugs. The changes of TCM syndrome score, serum estradiol(E2), progesterone(P), estrogen receptor(ER), progesterone receptor(PR), ki-67 and Bcl-2 before and after treatment were compared in the three groups. Results After treatment, the scores of TCM syndromes in the three groups were reduced, and group A was lower than group B and C. In group A and B, the expression of PR were increased(all P<0.05), the expression of Ki-67 and Bcl-2 were decreased(all P<0.05). The improvement of group A was better than that of group B and C(all P<0.05). Conclusion Huayu Sanjie therapy can reduce the clinical symptoms of patients with endometrial polyps after hysteroscopic resection and effectively prevent recurrence of endometrial polyps. Its mechanism may be related to that this method can improve the expression of endometrial PR and inhibit the over-expression of endometrial Ki-67 and Bcl-2 in endometrium.