Abstract: Objective To explore the clinical diagnostic value of serum gamma-glutamine transferase(γ-GT), cholinesterase(CHE), Apelin and visfatin for nonalcoholic fatty liver disease(NAFLD), and the effect of the changes of Apelin and visfatin on the levels of γ-GT and ChE in serum. Methods Fifty patients with NAFLD diagnosed in the Department of Gastroenterology in our hospital from June to September 2019 were selected as NAFLD group. A total of 50 healthy subjects who underwent physical examination in our hospital at the same time were selected as control group. Patients received venous blood and blood biochemical tests, and the differences in blood biochemical tests(γ-GT, CHE, Apelin, and Visfatin) were compared between the two groups. The Apelin(γ-GT, CHE) and Visfatin(γ-GT, CHE) four sets of random variables were used to perform pairwise-pairwise Person linear test. Results ① The levels of serum γ-GT, CHE and Apelin in the NAFLD group were higher than those in the control group. The serum visfatin level in the NAFLD group was lower than that in the control group(t=35.294, 29.373, 50.727, 37.009, all P<0.001). ② The level of Apelin in NAFLD group was significantly positively correlated with the changes of γ-GT(r=0.676) and CHE(r=0.699, all P<0.05). The visfatin level was negatively correlated with the γ-GT(r=-0.731), the CHE(r=-0.698, all P<0.05). Conclusion The levels of γ-GT, CHE, Apelin and visfatin in patients with NAFLD have reference value for the clinical diagnosis of NAFLD. Apelin is a possible inducer of NAFLD, while visfatin is a protective factor of NAFLD, and also has clinical value for the prevention and treatment of NAFLD.