Abstract: Objective To observe the application effect of continuous brain function monitoring in early diagnosis of neonatal hypoxic-ischemic encephalopathy. Methods Total 60 neonates with hypoxic-ischemic encephalopathy diagnosed in our hospital from June 2018 to May 2019 were selected in this study. They were divided into control group and observation group with 30 cases in each group. Continuous brain function monitoring was used in the observation group, and Transcranial Doppler(TCD) monitoring was used in the control group. The application effect of the two groups was compared. Results The sensitivity and specificity of the two groups were 90.00% and 86.67% in the observation group, 63.33% and 60.00% in the control group, respectively(all P<0.05). The difference of the brain function between the two groups was not statistically significant(P>0.05). The Vs and Vd of the neonates with hypoxic-ischemic encephalopathy increased in a way of time dependent way. Compared with the control group, the Vs and Vd of the observation group at 24 h and 48 h were significantly reduced(all P<0.05). There was no significant difference in Vs and Vd between 2 groups at 72 h(all P>0.05), and there was no significant difference in RI between 2 groups at different times(P>0.05). There was no interaction between the parameter groups and time(P>0.05). Conclusion Continuous brain function monitoring is an objective and reliable method for the monitoring of neonatal hypoxic-ischemic encephalopathy, which can improve the accuracy of monitoring, accurately reflect the state of illness, facilitate the later treatment and guarantee the life safety of the newborn.