Abstract: Objective To summarize Professor Cui Yun's experience of paired drugs and the three drugs pairing in treating oligoasthenospermia. Methods The clinical cases of oligoasthenospermia treated by Professor Cui Yun were collected. Professor Cui Yun's experience of paired drugs and the three drugs pairing in treating oligoasthenospermia were comprehensively analyzed on the basis of fully understanding the etiology and pathogenesis. The medication thinking and theory of Cui Yun's experience in treating oligoasthenospermia were discussed, and a case was given to prove it. Results Professor Cui Yun thought that the cause of oligozoospermia was closely related to kidney, liver, heart, spleen, lung organs, qi and blood, damp-heat and turbidity toxin. In view of the characteristics of pathogenesis, we taken paired drugs and the three drugs pairing such as "treatment of liver and kidney" "pleasant the heart and tranquilizing the mind" "smoothing the liver and regulating the stomach" "generation between the metal and water" "regulation of qi and blood", and "detoxification and dispel turbidity". "Nvzhenzi-Hanliancao" "Chaihu-Xuduan" "Shengdihuang-shanyao-Shanzhuyu" "Wuweizi-Fuling" "Yuanzhi-Shichangpu" "Dazao-Huangqin" "Shengguya-Shengmaiya" "Shanzha-Wuweizi" "Maidong-Wuweizi" "Huangqi-Dangshen-Danshen" "Danggui-Chuangxiong" "Diyu-Huzhang" "Guanzhong-Shengma-Xixiancao" were the most common used herb pairs and the three drugs pairing in practice. Professor Cui Yun was good at theoretical innovation in treating oligoasthenospermia based on syndrome differentiation according to the theory of liver and kidney. The treatment methods included that strengthening the body resistance to eliminate pathogenic factors, therapy of supplementation and draining and therapy of reinforcement and elimination in combination. The drugs for oligoasthenospermia therapy was warm but not dry, nourishing but not greasy. The experience of paired drugs and the three drugs pairing in treating oligoasthenospermia was not only profound but also the mechanism, method, prescription and drug of discriminating was unique. Conclusion The thought of Professor Cui Yun about mechanism and treatment of oligoasthenospermia is profound. It is worth learning and using for reference.