Abstract: Objective To explore the method and significance of cultivating humanistic qualities of general discipline students in standardized resident training. Methods A total of 29 general discipline students of grade 2015-2016 who were trained in the first affiliated hospital of Bengbu medical college were selected as research objects. The classroom teaching, special lectures, grassroots practice, clinical teaching were used to develop the humanistic quality training of the whole disciplines. Based on the questionnaire and scale survey of the 29 trainees before the training, after the training, the trainees will be surveyed and scaled. Self-designed open questionnaire, Caring Characteristics Scale(CAS), Caring Ability Inventory(CAI), SEGUE Framework(SEGUE) and General Doctor Competency Questionnaire(GDCQ) were used to evaluate the effects of humanistic quality training. Results The participation rate of the training of the humanities quality of the students is 100.0%. There were 89.7% of the general discipline students believed that cultivating humanistic quality is very important for clinical work. The knowledge of humanistic quality of general discipline students comes from the highest proportion of clinical teaching accounting for 81.6%, followed by grassroots practice and special lectures, accounting for 70.7% and 60.3%, respectively, while the proportion from classroom teaching is the lowest, accounting for 53.4%. Before and after the training of humanistic quality, the humanistic care consciousness, caring ability, doctor-patient communication ability and post competence of the general discipline students were significantly improved, and the differences were significant(all P<0.05). Conclusion Cultivating humanistic quality for all disciplines and training students can be carried out through classroom learning, special lectures, social practice, clinical practice, etc., and classroom teaching has the greatest proportion of the improvement of comprehensive humanistic quality.