Abstract: Objective To explore the effective management mode of Rh negative blood security by investigating the supply and regulation of Rh negative blood in Zhejiang Province. Methods The supply of RBC, the supply and the regulation of Rh negative RBC in Zhejiang province from 2014 to 2018 were analyzed retrospectively. Results In the whole province, the supply of RBC and Rh negative RBC, as well as the total amount of Rh negative RBC regulation among cities in the Zhejiang province showed an increasing trend year by year(r=0.987, 0.989, 0.949; P=0.002, 0.001, 0.051). The first two were highly correlated(r=0.979, P=0.004), while the latter two were not statistically significant(r=0.703, P=0.186). In the whole province, the proportion of Rh negative RBC supply in RBC supply increased year by year, which was 0.531%, 0.565%, 0.591%, 0.599% and 0.602% respectively. The RBC supply in each city showed an upward trend year by year, but fluctuated in Rh negative RBC supply and Rh negative RBC dose regulation between cities in the province, and there were great differences among cities. Conclusion The supply of Rh negative RBC in different cities is fluctuating, the overall supply in the province is increasing year by year, and the regulation within the province is becoming more and more active and important. Therefore, it is of great significance to strengthen regional linkage guarantee, establish and improve the cross regional coordination mechanism of Rh negative blood.