Abstract: Objective To investigate the stress response of patients undergoing otorhinolaryngologic surgery and the effect of clinical intervention. Methods From January to February 2017, 100 cases of otorhinolaryngologic surgery were included in the Department of Otolaryngology of Jiaxing first hospital to observe the occurrence of stress reaction and the effect of clinical intervention. Results The operation completed successfully, the average time was(21.23±5.66) min. No complications occurred in the follow-up. Pain comparison:the VAS pain scores at 1 hour after operation(before intervention) was(4.69±1.42) scores and in 1 th day, 3 th day and 7 th day(1 week) after operation were(2.75±1.03) score,(1.63±0.84) score,(0.59±0.45) score,the difference was statistically significant(F=308.951, P<0.001). There was statistically significant difference in VAS pain level at 1 hour after operation(before intervention) with Grade 0 of 10 cases, grade Ⅰ of 23 cases, grade Ⅱ of 54 cases, grade Ⅲ of 13 cases, and 7 th day(1 week) after operation with Grade 0 of 40 cases, grade Ⅰ of 49 cases, grade Ⅱ of 10 cases, grade Ⅲ of 1 case, the difference was statistically significant(Hc=67.920, P<0.001).The SAS scores before the operation and at the 1 th day, 3 th day and 7 th day(1 week) after operation were(56.42±9.78) score,(46.30±7.98) score,(40.12±10.47) score, and(33.25±8.45) score, respectively.There were statistically significant differences in SAS score(F=113.671, P<0.001). There SDS scores before the operation and at the 1 th day, 3 th day and 7 th day(1 week) after operation were(48.55±6.59) score,(46.20±5.33) score,(35.89±4.60) score, and(34.70±4.52) score, respectively. There were statistically significant differences in SDS score(F=175.614, P<0.001). Conclusion Most of the patients with otorhinolaryngologic surgery have stress reaction. The clinical intervention such as pain intervention and psychological counseling can reduce their bad mood, relieve and improve the postoperative pain feeling.