Abstract: Objective To explore the application and effects of specialist nurse-led multidisciplinary collaboration nursing model in promoting the comprehensive ability of nurses in Radiotherapy Department of Gynecological Oncology in cancer hospital. Methods A total of 34 formal nurses were selected, who were given routine nursing management(control group) from January to December 2017 and multi-disciplinary cooperation mode management(observation group) led by specialized nurses from January to December 2018. The ability to master the professional knowledge, the degree of mastering the disease, the understanding of MDT, the amount of regional nursing consultation and nursing satisfaction were compared between the two groups. Results The observation group:the image data interpretation(2.64±0.81), treatment guide application(2.25±0.76), special nursing guide(2.69±0.78), correct care of skin lesions(3.23±0.69), understanding of tumor stage(2.64±0.81), treatment means(2.82±0.62), nursing focus(2.91±0.77), scientific evaluation of nutrition(2.71±0.52) and psychology(2.53±0.59) and MDT comprehensive ability(2.82±0.62) were significantly higher than those of the control group[(1.94±0.64),(1.87±0.62),(2.31±0.65),(2.61±0.58),(2.20±0.76),(2.44±0.74),(2.52±0.61),(2.38±0.40),(2.21±0.55),(2.47±0.52)](all P<0.05). The nursing satisfaction(99.14±0.19) score was significantly higher than that of the control group(94.98±2.02), t=11.956, P<0.05. The amount of nursing consultation(42 cases) and the direction of consultation(9 cases) in the observation group were significantly increased compared with those in the control group(7 cases, 5 cases). Conclusion Specialist nurse-led multidisciplinary collaboration model can promote the comprehensive ability of nurses in specialized hospital.