Abstract: Objective To investigate the mental health status of standardized training of general practitioners in Nanjing, and to provide reference for further improvement of standardized general practice training. Methods A questionnaire survey was conducted among 110 general practitioners in Nanjing with the Symptom Checklist 90(SCL-90) and the self-made General Situation Questionnaire. Results The positive rate of SCL-90 of general practitioners in Nanjing was 24.5%, with a total average score of(121.51±30.69), which was lower than the national norm score of(129.96±38.76), t=-2.888, P<0.05. Apart from obsessive-compulsive factor, the average scores of eight factors, including somatization, interpersonal sensitivity, depression, anxiety, hostility, terror, paranoia and psychosis, were lower than those of the national norm, among which there were statistically significant differences in somatization, interpersonal sensitivity, depression, anxiety, hostility and paranoia. Mental health status of students whose training time longer than or equal to one year was better than that of students whose training time less than one year. The mental health status of the students who had completed the general theory study was better than that of the students who had not attended the general theory study. Conclusion The mental health status of Nanjing general practice standardized training physician is still good. Measures such as increasing students' professional interest, implementing the dual tutorial system for resident doctors and conducting group mental health education may effectively improve the mental health status of trained physicians.