Abstract: Objective To explore the application effect of four-step didactics in teaching of laparoscope simulator. Methods Total 132 clinical medical doctors receiving standardized training in the Training Center of the First Affiliated Hospital of Bengbu Medical College from May to December 2019 were divided into control group(n=66) and experimental group(n=66) according to random number table. The control group were taught with conventional didactics, and the experimental group were taught with four-step didactics. The teaching contents included three basic operations:transferring beans, stitching knots and cutting graphics under endoscope simulator. Three weeks later, the assessment combined with the classroom feedback questionnaire for teaching effect were analyzed and compared. Results Compared with the control group, the experimental group had advantages in objective score[(59.06±4.37) points vs.(57.39±4.45) points], subjective score[(19.73±1.36) points vs.(18.98±2.62) points] and total score[(78.79±4.40) points vs.(76.38±5.27) points], and the difference was statistically significant(P<0.05). The scores of the experimental group were significantly higher than those of the control group(Z=2.093, P=0.036). Conclusion The application of four-step didactics in laparoscopic simulator teaching can improve the basic skills, which is worth applying and popularizing in bed teaching.