Abstract: Objective To explore the application of case-based learning(CBL) combined with problem-based learning(PBL) and traditional teaching method(LBL) in the standardized training of general surgery residents. Methods From September 2016 to September 2018, 36 residents in General Surgery Department of Yijishan Hospital of Wannan Medical College were selected as subjects. They were randomly divided into experimental group(CBL + PBL group, 18 cases) and control group(LBL group, 18 cases). The differences of teaching effect and satisfaction between the two groups were compared. Results There was no significant difference between the two groups in average age, gender, admission examination results, original unit of students(all P<0.05). In basic theory examination, the average scores of resident trainees in the experimental group(47.1±1.4) and the control group(42.2 ±1.2) were significantly different(P<0.05). In skill operation assessment, the average score of the students in the experimental group(45.5±1.3) was significantly better than that in the control group(40.6±1.5), and the difference was statistically significant(P<0.05). The interest in learning, autonomous learning ability, clinical operation skills, team cooperation ability, literature review ability, clinical thinking ability of students in the experimental group were higher than those in the control group(all P<0.05). Conclusion CBL combined with PBL teaching method is helpful to deepen the understanding of general surgery theory knowledge of resident physicians, and to cultivate the ability of autonomous learning and problem solving. It is obviously superior to LBL teaching method and can effectively improve the standardized training effect of resident physicians in general surgery.