Abstract: Objective To study the diagnostic value of CD64 index of neutrophils, PCT and CRP in burn patients. Methods Total 117 patients with suspected burn infection admitted to our hospital from August 2018 to March 2019 were selected as the study subjects. According to the infection situation, the patients were divided into uninfected group(61 cases), infection group(39 cases) and sepsis group(17 cases). Another 40 healthy people in the same period were selected as control group. CD64 index, CD64 positive rate, PCT and CRP were detected. The expression levels of infection indicators in each group were compared. The indicators of gram-positive bacteria and gram-negative bacteria were compared. The ROC curve was drawn to compare the efficacy of infection indicators in diagnosis of burn infection and sepsis. Results The indicators of burn patients of the three groups were significantly higher than those in the control group(all P<0.05). The CD64 index, CD64 positive rate and PCT in the infected group were significantly higher than those in the uninfected group(all P<0.05). The indicators in the sepsis group were significantly higher than those in the infected group(all P<0.05). PCT of gram-negative bacterial infections was significantly higher than that of gram-positive bacterial infections(P<0.05). CD64 index has the best diagnostic efficiency for burn infection, with sensitivity 82.14%(46/56), specificity 88.52%(54/61) and Yoden index 0.707. Yoden index of CD64 index for sepsis(0.780) is the largest, and the AUC(0.896) for PCT is the highest. When CD64 index and PCT were combined to diagnose infection and sepsis, the sensitivity and Yoden index were higher than those of single index. Conclusion CD64 index combined with PCT has good sensitivity and specificity in the diagnosis of burn infection and sepsis, and has good clinical application prospects.