Abstract: Objective To assess the application value of preoperative blood inflammatory index neutrophil-lymphocyte ratio (NLR), platelet-lymphocyte ratio (PLR) and monocyte-lymphocyte ratio (MLR) in the prognosis of patients with glioma. Methods The clinical data of 236 patients with glioma treated at the Affiliated Hospital of Nantong University from January 2013 to December 2018 was analyzed retrospectively. The application value of preoperative NLR, PLR, MLR in prognosis of patients with glioma wad assessed by ROC curve, Kaplan-Meier survival curve, univariate and multivariate analysis. Results The expressions of NLR, PLR and MLR were correlated with tumor grade (all P<0.05). The best cutoff values for survival predict were 2.40, 127 and 0.24 respectively. The survival curve showed the median survival time in the high NLR group, high PLR group, and high MLR group was significantly shorter than that in the low NLR group, low PLR group, and low MLR group (all P<0.001). Multivariate analysis showed that preoperative NLR was an independent risk factor for predicting prognosis (P=0.008). Conclusion Preoperative blood inflammatory index NLR test is convenient and fast, which is an independent risk factor that affects the prognosis of glioma patients and has an important clinical value for prognosis of glioma patients.