Abstract: Objective To explore the clinical efficacy of Yellow soup of paeoniae combined with human chorionic gonadotropin in the treatment of female infertility caused by luteal phase defect and its influence on endometrial development, hormone levels and ovarian arterial blood rheology parameters. Methods Eighty-six cases of luteal infertility women treated in Wenzhou City Hospital of Integrated Traditional Chinese and Western Medicine, from January 2017 to March 2019, were divided into 2 groups by random number method, 43 cases in each group. The main follicles were detected human chorionic gonadotropin, and the observation group was given the treatment of Yellow soup of paeoniae and human chorionic gonadotropin. The two groups were treated for one course of treatment, and the treatment was followed for three months. After treatment, the clinical efficacy and symptom score, endometrial thickness and changes of hormone levels in ovarian arterial blood rheology parameters (pulsation index, resistance index, end-diastolic velocity and peak systolic velocity) between observation group and control group were compared. Results After treatment, the total effective rate of the control group was 79.07%, the total effective rate of the observation group was over 95.34%, and the total effective rate of the observation group was higher than that of the control group (χ²=5.108, P=0.024). The total effective rate of the observation group was significantly higher than that of the control group (P<0.05). After treatment, the uterus thickness and main follicle diameter of both groups were significantly increased (all P<0.05), and the endometrial thickness and main follicle diameter of the observation group were significantly higher than the control group (P<0.05). After treatment, the pulsation index, end-diastolic velocity and peak systolic velocity of the two groups were improved (all P<0.05), and the improvement degree of the observation group was significantly better than that of the control group (P<0.05). Conclusion The use of Yellow soup of paeoniae combined with human chorionic gonadotropin in the treatment of luteal phase defect infertility has a significant effect. The clinical symptom score is significantly improved, the regulation function of the hypothalamic-pituitary-ovarian axis is improved, and the function of the corpus luteum and endometrial thickness are improved, effective regulation ovarian artery blood flow parameters.