Abstract: Objective Patients with early stage gynecological tumors need indwelling catheterization during operation to ensure smooth urinary drainage during and after surgery. However, difficulty urinating or even urinary retention often occur due to various reasons after surgery. This study aims to explore the effect of intermittent pinch valve combined with anal levator movement on urinary function after urinary catheter removal in gynecologic tumor patients. Methods Ninety patients with gynecologic tumors were enrolled from January 2018 to June 2019 in this study. Patients were randomly divided into experimental group (45 cases) and control group (45 cases) according to the random number table method. Patients in control group were nursed according to the nursing routine of indwelling catheterization, and that in experimental group were nursed with intermittent pinch tube combined with anal movement on the basis of routine nursing, and the catheter was removed when the bladder was full or the patient complained of obvious urinary intention. Rank sum test was used to compare the waiting time for the first urination after extubation. Chi-square test was used to compare the distribution of urinary tract irritation and urination. Results The first urination time in control group was[105.0(35.2,210.4)]min after extubation, and the experimental group was[30.0(10.5,65.7)]min, the difference was statistically significant (U=506.000; P<0.01). Totally, 3 (6.7%) patients in experimental group had urinary tract irritation signs, and 12 (26.67%) in the control group had urinary tract irritation signs. The chi-square value was 6.480, and the difference was statistically significant (P<0.05). Totally, 43 (95.56%) patients in experimental group vomited spontaneously after surgery, 2 (4.44%) patients needed to induce urination, and no patients needed re-catheterization. Totally, 34 (75.56%) patients in the control group spontaneously urinate after surgery, and 7 (15.55%) patients needed induce urination, and 4 (8.89%) needed to urinate again. The chi-square value was 7.830, and the difference was statistically significant (P<0.05). Conclusion Intermittent pinch tube combined with levelling anus exercise, when the bladder filling or the patient complained of obvious urinary tract, the urethral tube can be removed, which can significantly reduce the waiting time for the first urination, promote the patient to resume spontaneous urination as soon as possible, reduce the chance of re-conducting. The nursing intervention measures are simple and effective, worthy of clinical promotion and application.