Abstract: Objective To explore the clinical effect of warm acupuncture on knee osteoarthritis(KOA) and its influence on the changes of patients' score of osteoarthritis(WOMAC), health survey(SF-36) and pain visual analogue(VAS). Methods Total 80 patients with KOA in Wenzhou Traditional Chinese Medicine Hospital from January to October 2018 were selected as research object, who were divided into control group and observation group according to the random number table method, 40 cases in each group. The control group was treated with conventional acupuncture, and the observation group was treated with warm acupuncture. The two groups needed continuous treatment for 28 d. The curative effects of the two groups were recorded. Before treatment, immediately after treatment and 6 months after treatment, VAS score and WOMAC score were used to evaluate the improvement of symptoms in the two groups. SF-36 score was used to evaluate the changes of quality of life(QOL). Results The total effective rate of KOA in the observation group was 90.0%, which was significantly higher than that in the control group(50.0%), the difference was statistically significant(χ²=15.238, P<0.001). The VAS score, WOMAC score and SF-36 score of the two groups were significantly improved at each time point after treatment, and the difference was statistically significant(all P<0.05). VAS scores, WOMAC scores and SF-36 scores in the observation group(3.20±0.98, 10.96±1.89, 96.83±2.96) were significantly better than those of the control group(4.30±0.99, 27.56±7.20, 80.03±3.20), and the difference was statistically significant(all P<0.01). Conclusion The warm acupuncture has good clinical treatment effect for treating the KOA. It can effectively relieve the pain of the patient and improve the activity of the bone joint of the patient, improve the quality of life of the patient, and has the clinical application value.