Abstract: Objective To analyze the relationships between lncRNA GAS5 and PTEN protein expressions and endothelial function in elderly patients with hypertension. Methods A total of 87 elderly hypertensive patients admitted to Jinhua Central Hospital from May 2018 to May 2019 were selected, and another 87 healthy persons were selected as control group during the same period. Real-time fluorescence quantitative(qRT-PCR) was used to detect the expression of lncRNA GAS5, and ELISA was used to detect the expression of PTEN. Meanwhile, the change rate of vascular diameter(FMD), systolic blood pressure(SBP) and diastolic blood pressure(DBP) were measured by electronic sphygmomanometer, and endothelium-dependent vasodilation rate(FMD) was measured by ultrasound diagnostic instrument, and the correlations between GAS5 and PTEN levels and the above indexes were analyzed. Results Systolic blood pressure and diastolic blood pressure in elderly patients with hypertension were significantly higher than those in the control group(all P<0.05). The level of vWF in elderly patients with hypertension was significantly higher than that in control group(P<0.05), and the FMD value and NO level in elderly patients with hypertension were significantly lower than those in control group(all P<0.05). The levels of serum lncRNA GAS5 and PTEN protein in elderly patients with hypertension were significantly lower than those in the control group(all P<0.05). Serum lncRNA GAS5 and PTEN protein levels were positively correlated with NO levels in elderly patients with hypertension(r=0.563, P=0.005; r=0.507, P=0.006), and negatively correlated with vWF(r=-0.457, P=0.004; r=-0.426, P=0.008). There was a positive correlation between serum GAS5 and PTEN level in elderly patients with hypertension(r=0.484, P<0.05). Conclusion The expressions of GAS5 and PTEN in serum of elderly patients with hypertension are down-regulated, which are related to the injury of vascular endothelial cells and the decrease of vascular relaxation function.