Abstract: Objective Build a "mobile medical assistant" stage dynamic missionary education model, and apply it to patients with in vitro fertilization and embryo transfer, To further improve the quality of health education in reproductive centers. Methods Total 340 patients undergoing IVF-embryo transfer admitted to the Reproductive Center of the Second Affiliated Hospital of Wenzhou Medical University were selected by convenient sampling. According to the time of admission, they were divided into control group and observation group, 170 cases in each group. The control group performed routine mission, and the observation group based on the control group combined with the "mobile assistance doctors" module to implement periodic dynamic mission. The intervention effects of the two groups were compared. Results At the pregnancy confirmation day, the scores of anxiety, depression and negative coping style in the observation group were(36.46±3.79) points,(35.90±3.39) points,(22.15±4.48) points, significantly lower than the control group [(40.07±4.43) points,(40.84±4.17) points,(25.09±5.13) points], and the observation group's positive coping style score was(40.15±5.56) points, significantly higher than the control group(37.53±6.02) points, the differences were statistically significant(all P<0.05). At the embryo transfer day, the cortisol level in the observation group was(49.12±4.11) pg/mL, significantly lower than that in the control group(52.30±4.53) pg/mL, the difference was statistically significant(all P<0.05). At the pregnancy confirmation day, the health knowledge awareness rate in the observation group(90.59%) was significantly higher than that in the control group(80.59%), and the health education satisfaction of the observation group(95.88%) was significantly higher than that of the control group(89.41%), the differences were statistically significant(all P<0.05). Conclusion "Mobile assistance doctors" stage dynamic mission can significantly improve the negative emotions, psychological stress and coping styles of patients with IVF-embryo transfer, and improve patient satisfaction.