Abstract: Objective To investigate Human papilloma virus(HPV) prevalence and genotype distribution of males whose female partners with cervical HPV infection and the HPV type-specific concordance between heterosexual couples. Methods A total of 443 heterosexual couples, among whom female partners had cervical HPV infection, were enrolled in our hospital from November 2017 to April 2019. The swab samples from males' external genital epithelial cells and females' cervical exfoliated cells were collected and 27 HPV subtypes both high-risk and low-risk types were detected. The results were analyzed. Results Among 443 males, HPV infection was positive in 216 with an infective rate of 48.76%. The top three HPV subtypes in males were 53, 52, 16, while the top three HPV subtypes among their wives also were 53, 16, 52. The prevalence of low-risk HPV subtypes between couples was different, the top two in men were HPV43, 61, while HPV81, 6 in women. Among 216 both HPV positive sexual partners the complete and partial concordance rate of HPV were 26.39%(57/216) and 36.11%(78/216), the total concordance rate was 62.50%(135/216), most of concordant subtype was single type, especially in high-risk HPV. Conclusion The HPV infective rate of males' external genital was slightly high among whose female partners had cervical HPV infection. The concordance rate of HPV subtypes in which both couples had HPV infection was high, especially in high-risk subtypes. We recommend to detect HPV among males whose female partners had cervical HPV infection and to cure, so that to improve the HPV clearance rate.