Abstract: Objective To understand the current situation of mental workers' occupational stress, and to discuss the factors affecting mental workers' occupational stress. Methods From 2018 to 2020, a total of 1 260 city's medical staff, teachers, ordinary civil servants, accountants, administrators, designers and other knowledge workers were randomly selected as the research object by random cluster sampling method. The occupational stress inventory(OSI-R) was used to investigate the occupational stress status mental labor population. The t test, analysis of variance, and logistic regression analysis were used to analyzed current situation and influence factors of occupational stress. Results The scores of mental workers in the occupational task questionnaire(except the working environment), the individual stress response questionnaire, the individual coping resources questionnaire and the three dimensions were all higher than the norm. Male, graduate student, high salary, 10-20 years of service, administrative position, night shift frequency had high occupational task score(all P<0.05). Male, length of service 10-20 years, graduate degree, working time >40 hours per week, high frequency of night shift, intermediate job title, had strong stress response(all P<0.05). The scores of coping resources were higher in males, undergraduates, junior professional titles and short working years(all P<0.05). The results of logistic regression analysis showed that gender, length of service, educational background and frequency of night shift were the risk factors affecting the occupational stress of mental workers. Conclusion Gender, length of service, frequency of night shift and educational background are the main influence factors for the serious degree of occupational stress of mental workers.