Abstract: Objective To investigate the correlation of plasma High-sensitivity C-reactive protein(hs-CRP), matrix metalloproteinase-9(MMP-9) and lipoprotein-related phospholipase A2(Lp-PLA2) levels with neurological impairment, cerebral infarction volume and carotid artery plaque stability in patients with acute cerebral infarction(ACI). Methods A total of 120 ACI in patients(ACI group) who were admitted in the Affiliated Hospital of Hangzhou Normal University from June 2017 to February 2020 were selected, and 60 healthy people for physical examination during the same period were selected as health control group. The levels of hs-CRP, MMP-9 and Lp-PLA2 in different groups were compared, and the Spearman Method was used to analyze the correlation. Results The levels of hs-CRP, MMP-9 and Lp-PLA2 in the ACI group were significantly higher than those in the healthy control group(t=20.364, 22.115, 14.163, all P<0.05); the levels of hs-CRP, MMP-9 and Lp-PLA2 in the mild, moderate, and severe neurological deficit groups increased sequentially, with significant differences(F=171.283, 82.137, 278.610, all P<0.05); the levels of hs-CRP, MMP-9 and Lp-PLA2 in the small, moderate, and severe infarct focus group increased sequentially, with significant differences(F=100.672, 59.707, 134.199, all P<0.05); the levels of hs-CRP, MMP-9 and Lp-PLA2 in the non-, stable, and unstable plaque groups increased sequentially, with significant differences(F=50.120, 34.056, 67.255, all P<0.05). The levels of MMP-9, hs-CRP and Lp-PLA2 in ACI patients were positively correlated with neurological deficits, cerebral infarction volume, and carotid plaque Carotid stability(all P<0.05). Conclusion The more unstable the plaque nature, the larger the cerebral infarction volume and the higher the neurological function defect degree were, the higher the serum HS-CRP, MMP-9 and LP-PLA2 levels were.