Abstract: Objective To explore the effect of triacetyl resveratrol(TRES) on apoptosis of human promyelocytic leukemia HL-60 cells. Methods After treatment with TRES in different concentrations(5, 12.5, 25, 50, 75 μmol/L) for indicated times(12, 24, 48 h), the proliferation of HL-60 cells were analyzed by Alamar blue assay. Annexin V-FITC/PI staining were conducted to detect apoptosis induced by TRES(5, 25, 50 μmol/L) for different times(12, 24 h). The expression of STAT3, pSTAT3, Bcl-2, Bax and Cleaved caspase-3 in HL-60 treated by TRES(5, 25, 50 μmol/L) for 24 h were detected by Western blotting. Results The results of Alamar blue showed that TRES had inhibitory effect on HL-60. The Annexin V-FITC/PI double staining showed that TRES induced apoptosis of HL-60 cells. With the increase of TRES concentration, the expression of STAT3 and Bax did not change significantly, the expression of pSTAT3 was significantly down regulated, the expression of Bcl-2 and Cleaved caspase-3 was significantly up-regulated, and the ratio of Bcl-2/Bax was down regulated, which eventually led to apoptosis. Conclusion TRES inhibits HL-60 cell proliferation, and induces apoptosis of HL-60 cells through STAT3-Bcl-2/Bax-caspase-3 pathway.