Abstract: Objective To explore the effect of whole process quality control management in disinfection management of supply room and its effect on hospital infection control. Methods The supply room of Quzhou Hospital of traditional Chinese Medicine began to implement the whole process quality control management in disinfection management from July 2016. A total of 25 012 cleaning, disinfection and sterilization articles in the supply room before the implementation of the whole process quality control(from July in 2014 to June in 2016) and 27 885 cleaning, disinfection and sterilization articles in the supply room after the implementation(from July 2016 to June 2018) were statistically analyzed. The qualification rate and nosocomial infection rate of sterilized articles in the supply room were compared before implementation and after implementation. Results Before the implementation of the whole process quality control management, the qualified rate of disinfection, sterilization, aseptic packaging and aseptic distribution were 83.0%, 86.0%, 86.0% and 85.0% respectively. After the implementation of the whole process quality control management, the qualified rate of disinfection, sterilization, aseptic packaging and aseptic distribution were 97.5%, 100.0%, 100.0% and 100.0% respectively. After the implementation, the qualified rates of sterilization, aseptic packaging and aseptic distribution were all increased(χ²=3 269.004, 4 181.060, 4 181.060, 4 502.324; all P<0.05). The total incidence of nosocomial infection(3.25% vs. 1.76%) decreased after the implementation of the whole process quality control management(χ²=120.451, P<0.05). There were significant differences in the infection rate of the surgical site/incision(1.57% vs. 0.27%) and the respiratory system(1.14% vs. 0.93%) before and after the implementation of the whole process quality control management(χ²=255.925, 5.956, all P<0.05). Conclusion The whole process quality control management in disinfection management of the supply room can improve the qualified rate of sterilization articles and reduce the incidence of hospital infection.