Abstract: Objective To analyze the application value of hemorheology test in the diagnosis of cardiovascular diseases, and to explore the correlation between hemorheology indexes and the occurrence and development of cardiovascular diseases. Methods A total of 62 cases of cardiovascular disease patients in our hospital from June 2019 to June 2020 were selected and included in the study group. Meanwhile, 62 healthy volunteers who underwent routine physical examination in our hospital at the same time were selected as the control group. The whole blood high shear viscosity, whole blood low shear viscosity, plasma viscosity, erythrocyte aggregation index, erythrocyte rigidity index, red blood cell fineness index were measured by automatic hemorheology analyzer. The hemorheology indexes such as cell deformation index, hematocrit, erythrocyte sedimentation rate, plasma fibrinogen were compared between the two groups. Pearson correlation analysis method was used to analyze the correlation between hemorheology indexes and cardiovascular disease. Results Before treatment, the whole blood high shear viscosity, whole blood low shear viscosity, erythrocyte aggregation index, erythrocyte rigidity index, erythrocyte deformation index, hematocrit and plasma fibrinogen test levels of the study group were higher than those of the control group, and the ESR test level was lower than that of the control group(P<0.05). The plasma viscosity of the study group before treatment was similar to that of the control group(P>0.05). After treatment, the above indexes in the study group were compared with those in the control group(all P>0.05). After treatment, the whole blood high shear viscosity, whole blood low shear viscosity, erythrocyte aggregation index, erythrocyte rigidity index, erythrocyte deformation index, hematocrit and plasma fibrinogen test levels in the study group were lower than those before treatment, and the ESR test level was higher than that before treatment(P<0.05). The plasma viscosity was similar to that before treatment(P>0.05). Pearson correlation analysis showed that cardiovascular disease was positively correlated with whole blood high shear viscosity, whole blood low shear viscosity, erythrocyte aggregation index, erythrocyte rigidity index, erythrocyte deformation index, hematocrit and plasma fibrin, and negatively correlated with ESR, but not with plasma viscosity. Conclusion Hemorheology indexes are abnormal expression in cardiovascular diseases. Hemorheology test can be used as an auxiliary reference for clinical diagnosis of cardiovascular diseases, and it is of great significance to improve the accuracy of the diagnosis results.