Abstract: Objective To strengthen the quality control and continuous improvement of disinfection supply center and analyze its role in infection prevention and control. Methods In August 2018, strengthened quality monitoring and continuous improvement activities were carried out in the disinfection supply center of Shaoxing Seventh People's hospital. The hospital infection rate, B-D test qualification rate of sterilized articles, qualified rate of work quality and delivery time of articles were compared before the implementation of enhanced quality control and continuous improvement activities(May 2017-May 2018) and after(August 2018-August 2019). Results After implementation, the incidence of nosocomial infection in patients treated with medical devices issued by the disinfection supply center was 1.1%, lower than 2.6% before implementation, and the pass rate of B-D experiment was 98.8%, higher than 95.4% before implementation, with statistically significant difference(all P<0.05). The qualified rate of work quality of the disinfection supply center after implementation, including cleaning quality, disinfection and sterilization, packaging quality, recovery, acceptance, classification and distribution, was higher than that before implementation, and the difference was statistically significant(all P<0.05). After implementation, the delivery time of conventional goods and urgently needed goods was(45.25±10.71) min and(9.64±1.17) min, both shorter than that before implementation(51.26±10.33) min and(12.37±2.28) min, with statistically significant difference(all P<0.05). Conclusion Strengthening quality control and continuous improvement can effectively improve the prevention and control of infection in the disinfection supply center, improve the qualified rate of goods in the supply room, and shorten the delivery time of goods, which is worthy of clinical application.