内皮祖细胞移植对百草枯中毒所致急性肺损伤大鼠炎性因子表达的影响

靳妍; 刘志; 孙宁; 杨宏达; 才权

doi:10.16766/j.cnki.issn.1674-4152.001715

内皮祖细胞移植对百草枯中毒所致急性肺损伤大鼠炎性因子表达的影响

doi: 10.16766/j.cnki.issn.1674-4152.001715

中国医科大学附属第一医院急诊科，辽宁沈阳 110001

基金项目:

国家自然科学基金项目 81772053

详细信息

通讯作者:
刘志，E-mail：yan753p@126.com

中图分类号: R139.3 R563
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- 文章访问数: 138
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- 被引次数: 0
出版历程
- 收稿日期: 2020-05-27
- 网络出版日期: 2022-02-19

Effects of endothelial progenitor cell transplantation on the expression of inflammatory factors in a rat model of acute lung injury caused by paraquat poisoning

Emergency Department, the First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, China

摘要

摘要: 目的分析内皮祖细胞移植对百草枯中毒所致急性肺损伤大鼠模型炎症因子表达的影响，为内皮祖细胞移植在临床中的应用提供参考。方法选取60只雄性SD大鼠作为实验动物，采取随机数字表法将大鼠分为对照组、百草枯组(模型组)和内皮祖细胞移植组(观察组)，各20只，比较3组大鼠的肺组织损伤情况及血清炎性因子等指标的表达水平。结果 3组大鼠的肺组织损伤评分和湿干重比比较差异有统计学意义(F=12.336、10.571，均P＜0.05)，模型组大鼠的肺组织损伤评分和湿干重比均高于观察组和对照组(均P＜0.05)，但观察组和对照组比较差异无统计学意义(均P＞0.05)；3组大鼠肺血清炎性因子表达水平比较差异有统计学意义(F=23.305、26.271、18.814、20.056，均P＜0.05)，观察组和模型组大鼠的血清炎性因子表达水平均高于对照组(均P＜0.05)，但观察组的血清炎性因子表达水平低于模型组(均P＜0.05)；3组大鼠的氧化应激指标比较差异有统计学意义(F=8.326、14.505，均P＜0.05)，模型组大鼠的丙二醛(MDA)明显高于观察组和对照组(均P＜0.05)，且超氧化物歧化酶(SOD)明显低于观察组和对照组(均P＜0.05)，但观察组和对照组氧化应激指标比较差异无统计学意义(均P＞0.05)。结论内皮祖细胞移植可以降低百草枯中毒所致急性肺损伤大鼠模型炎症因子表达，具有较好的疗效。
- 内皮祖细胞 /
- 百草枯 /
- 急性肺损伤 /
- 炎症因子
Abstract: Objective To analyse the effect of endothelial progenitor cell transplantation on the expression of inflammatory factors in a rat model of acute lung injury caused by paraquat poisoning and to provide references for the clinical application of endothelial progenitor cell transplantation. Methods Sixty male SD rats were selected as experimental animals, and the rats were divided into the control group, paraquat group (model group) and endothelial progenitor cell transplantation group (observation group) by a random number table method. The tissue damage and expression level of serum inflammatory factors and other indicators were also compared. Results The lung tissue damage score and wet-dry weight ratio of the three groups of rats were significantly different (F=12.336, 10.571, all P < 0.05). The lung tissue damage score and wet-dry weight ratio of the model group were higher than those of the observation and control groups (all P < 0.05), but no significant difference was found between the observation and control groups (all P>0.05). The lung serum inflammatory factor expression levels of the three groups of rats were significantly different (F=23.305, 26.271, 18.814, 20.056, all P < 0.05), and the expression levels of the serum inflammatory factors in the observation and model groups were higher than those in the control group (all P < 0.05), but the expression level of the serum inflammatory factors in the observation group was lower than that in the model group (all P < 0.05). The oxidative stress indicators of the three groups of rats were significantly different (F=8.326, 14.505, all P < 0.05). The MDA of the model group was significantly higher than that of the observation and control groups (all P < 0.05), and SOD of the model group was significantly lower than that of the observation and control groups (all P < 0.05), but the observation and control groups had no significant difference in oxidative stress indicators (all P>0.05). Conclusion Endothelial progenitor cell transplantation can reduce the expression of inflammatory factors in a rat model of acute lung injury caused by paraquat poisoning, which has a definite effect.
- Endothelial progenitor cells /
- Paraquat /
- Acute lung injury /
- Inflammatory factors

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图 1 大鼠肺组织HE染色结果(×400)

注：A为对照组；B为模型组；C为观察组。

下载: 全尺寸图片幻灯片

表 1 3组大鼠肺组织损伤评分比较(x ±s，分)

组别	只数	肺组织损伤评分
观察组	20	1.36±0.33
模型组	20	10.03±0.75^ab
对照组	20	0.59±0.11
F值		12.336
P值		0.015
注：与对照组比较，^aP＜0.05；与观察组比较，^bP＜0.05。

下载: 导出CSV

表 2 3组大鼠肺组织湿干重比比较(x ±s)

组别	只数	肺组织湿干重比
观察组	20	4.76±1.02
模型组	20	9.26±1.89^ab
对照组	20	3.82±0.81
F值		10.571
P值		0.020
注：与对照组比较，^aP＜0.05；与观察组比较，^bP＜0.05。

下载: 导出CSV

表 3 3组大鼠的血清炎性因子表达水平比较(x ±s)

组别	只数	TNF-α(pg/mL)	IL-6(ng/mL)	IL-10(ng/mL)	IL-1β(ng/mL)
观察组	20	126.34±12.23^a	152.26±23.36^a	85.71±5.62^a	85.53±7.02^a
模型组	20	202.34±25.82^ab	352.18±52.71^ab	95.54±12.79^ab	142.23±18.83^ab
对照组	20	42.11±4.32	80.22±4.45	47.18±2.36	57.31±4.09
F值		23.305	26.271	18.814	20.056
P值		< 0.001	< 0.001	< 0.001	< 0.001
注：与对照组比较，^aP＜0.05；与观察组比较，^bP＜0.05。

下载: 导出CSV

表 4 3组大鼠肺组织的氧化应激指标比较(x ±s)

组别	只数	MDA(nmol/mg)	SOD(U/mg)
观察组	20	1.02±0.29	26.91±2.59
模型组	20	2.29±0.57^ab	15.54±2.24^ab
对照组	20	0.83±0.07	28.72±1.23
F值		8.326	14.505
P值		0.031	0.011
注：与对照组比较，^aP＜0.05；与观察组比较，^bP＜0.05。

下载: 导出CSV

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