轻度胃肠炎伴婴幼儿良性惊厥患儿血清γ干扰素、硫化氢水平变化及其临床意义

张玲; 屠友权; 方春艳; 黄明海

doi:10.16766/j.cnki.issn.1674-4152.001780

轻度胃肠炎伴婴幼儿良性惊厥患儿血清γ干扰素、硫化氢水平变化及其临床意义

doi: 10.16766/j.cnki.issn.1674-4152.001780

宁波市妇女儿童医院小儿神经内科，浙江宁波 315000

基金项目:

浙江省医药卫生项目 2020KY883

详细信息

通讯作者:
黄明海，E-mail：hmhai432@126.com

中图分类号: R720.597 R446
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- 被引次数: 0
出版历程
- 收稿日期: 2020-08-06
- 网络出版日期: 2022-02-19

Changes and clinical significance of serum IFN-γ and H₂S levels in children with benign infantile convulsion associated with mild gastroenteritis

Department of Pediatric Neurology, Women and Children's Hospital in Ningbo, Ningbo, Zhejiang 315000, China

摘要

摘要: 目的探究轻度胃肠炎伴婴幼儿良性惊厥(BICE)患儿血清γ干扰素(IFN-γ)、硫化氢(H₂S)水平变化及临床意义，探究其在BICE的发生及疾病进展中的作用。方法以2017年3月—2019年7月在宁波市妇女儿童医院治疗的BICE患儿83例为观察组，并以同期健康体检者65例为对照组。分别采用ELISA法、分光光度法检测血清IFN-γ水平及H₂S水平，分别采用Pearson检验、Spearman检验分析患儿血清IFN-γ、H₂S水平及与病情严重程度的相关性；采用ROC曲线分析血清IFN-γ、H₂S水平对BICE的诊断价值。结果观察组血清H₂S、IFN-γ水平为(30.15±6.09)μmol/L、(5.68±1.13)μg/L，对照组为(43.28±8.34)μmol/L、(4.25±0.92)μg/L(t=11.066、8.277，均P < 0.001)；患儿血清IFN-γ水平与BICE的发病及病情严重程度呈正相关(P < 0.05)，而H₂S水平与BICE的发病及病情严重程度呈负相关(P < 0.05)；血清IFN-γ、H₂S水平诊断BICE的AUC为0.785、0.895。结论 BICE患儿血清IFN-γ、H₂S水平与病情严重程度有关，且血清IFN-γ、H₂S水平对BICE具有诊断价值。
- 轻度胃肠炎伴婴幼儿良性惊厥 /
- 干扰素γ /
- 硫化氢 /
- 病情严重程度
Abstract: Objective To explore the changes and clinical significance of serum interferon-γ (IFN-γ) and hydrogen sulfide (H₂S) levels in children with benign infantile convulsion associated with mild gastroenteritis (BICE), as well as to explore their roles in the occurrence and development of BICE. Methods A total of 83 BICE children who were treated in Women and Children's Hospital in Ningbo from March 2017 to July 2019 were enrolled as observation group, whereas another 65 healthy controls during the same period were enrolled as control group. The levels of serum IFN-γ and H₂S were detected by ELISA and spectrophotometry. The correlation between serum IFN-γ and H₂S levels and the correlation between the two indices and severity of BICE were analysed by Pearson and Spearman tests. The diagnostic value of serum IFN-γ and H₂S levels for BICE was analysed by ROC curves. Results The levels of serum H₂S and IFN-γ in the observation group were significant different from those in the control group [(30.15±6.09) μmol/L, (5.68±1.13) μg/L vs. (43.28±8.34) μmol/L, (4.25±0.92) μg/L, t=11.066, 8.277, all P < 0.001].They were also correlated with the severity of BICE (all P < 0.05). The AUC values of serum IFN-γ and H₂S levels for diagnosis of BICE were 0.785 and 0.895, respectively. Conclusion The levels of serum IFN-γ and H₂S are relevant in BICE children and associated with disease severity. The serum IFN-γ and H₂S levels are of diagnostic value for BICE.
- Benign infantile convulsion associated with mild gastroenteritis /
- Interferon-γ /
- Hydrogen sulfide /
- Disease severity

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图 1 血清IFN-γ、H₂S水平诊断BICE的ROC曲线分析

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表 1 观察组及对照组血清IFN-γ、H₂S水平比较(x ±s)

组别	例数	H₂S(μmol/L)	IFN-γ(μg/L)
观察组	83	30.15±6.09	5.68±1.13
对照组	65	43.28±8.34	4.25±0.92
t值		11.066	8.277
P值		< 0.001	< 0.001

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表 2 急性期及恢复期BICE患儿血清IFN-γ、H₂S水平比较(x ±s)

组别	例数	H₂S(μmol/L)	IFN-γ(μg/L)
急性期	47	38.46±7.83	5.83±1.17
恢复期	36	47.31±7.47	5.09±1.07
t值		5.205	2.962
P值		< 0.001	0.004

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表 3 患儿血清IFN-γ、H₂S水平与BICE发病及病情严重程度的相关性分析

指标	BICE发病		病情严重程度
指标	r值	P值	r值	P值
IFN-γ	0.490	< 0.001	0.329	0.002
H₂S	-0.680	< 0.001	-0.523	< 0.001

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表 4 血清IFN-γ、H₂S水平对BICE的诊断价值分析

指标	截点值	AUC	SE	95% CI
IFN-γ	4.74 μg/L	0.785	0.037	0.710~0.848
H₂S	36.51 μmol/L	0.895	0.026	0.834~0.940

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表 5 IFN-γ高、低水平组和H₂S高、低水平组BICE患儿的一般资料比较(例)

组别	例数	年龄(≤2岁)	性别(男)	惊厥发作次数(≥2次)	惊厥持续时间(≥5 min)	发病季节(冬春)	惊厥发作类型(强直阵挛发作)	发病期(急性期)
IFN-γ高水平组	42	21	25	25	22	28	30	31
IFN-γ低水平组	41	19	22	12	12	25	32	11
χ²值		0.112	0.291	7.687	4.583	0.291	0.481	18.319
P值		0.738	0.590	0.006	0.032	0.589	0.488	< 0.001
H₂S高水平组	50	24	28	10	9	34	40	12
H₂S低水平组	33	16	19	27	25	19	22	30
χ²值		0.002	0.020	32.662	27.422	0.936	1.870	17.161
P值		0.965	0.887	< 0.001	< 0.001	0.333	0.171	< 0.001

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