糖皮质激素受体激动剂对神经病理性疼痛大鼠脊髓胶质纤维酸性蛋白及炎性因子表达的影响

杨慧芳; 赵莉; 逯岭松; 吴艳辉

doi:10.16766/j.cnki.issn.1674-4152.001817

糖皮质激素受体激动剂对神经病理性疼痛大鼠脊髓胶质纤维酸性蛋白及炎性因子表达的影响

doi: 10.16766/j.cnki.issn.1674-4152.001817

1.
浙江大学医学院附属杭州市第一人民医院麻醉科，浙江杭州 310006
2.
浙江大学医学院附属杭州市第一人民医院中心实验室，浙江杭州 310006

基金项目:

浙江省医药卫生一般项目 2016KYA156

详细信息

通讯作者:
杨慧芳，E-mail：yhf5066@sina.com

中图分类号: R441.1 R-33
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- 被引次数: 0
出版历程
- 收稿日期: 2020-02-25
- 网络出版日期: 2022-02-19

Expression of GFAP and inflammatory factors in spinal cord of neuropathic pain rats treated with glucocorticoid receptor agonists

1.
Department of Anesthesiology, Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310006, China

摘要

摘要: 目的探讨糖皮质激素受体激动剂对神经病理性疼痛大鼠脊髓胶质纤维酸性蛋白(GFAP)及炎性因子表达的影响。方法 20只SD大鼠随机分为对照组、假手术组、模型组、治疗组，每组5只，模型组和治疗组应用坐骨神经慢性压迫性损伤法建立神经病理性疼痛模型，假手术组仅暴露大鼠坐骨神经、不结扎，治疗组鞘内注射地塞米松，其他三组鞘内注射等容量生理盐水，连续注射7 d。于术前1 d和术后3、7 d采用Von Frey纤维丝测定机械痛阈、热刺痛仪测定热痛阈，术后7 d处死大鼠，采用Western blotting检测脊髓GFAP蛋白表达水平，RT-PCR法检测脊髓炎性因子mRNA表达水平。结果治疗组术后3、7 d的机械痛阈和热痛阈较对照组和假手术组显著降低(P < 0.05)，但较模型组显著升高(P < 0.05)。模型组和治疗组脊髓GFAP蛋白表达水平较对照组和假手术组均显著升高(P < 0.05)，但治疗组脊髓GFAP蛋白表达水平显著低于模型组(P < 0.05)。模型组和治疗组脊髓TNF-α、IL-1β mRNA表达水平较对照组和假手术组均显著升高(P < 0.05)，但治疗组脊髓TNF-α、IL-1β mRNA表达水平显著低于模型组(P < 0.05)。结论糖皮质激素受体激动剂可明显减轻神经病理性疼痛大鼠的痛敏反应，其作用机制可能与下调脊髓GFAP及下游炎性因子表达有关。
- 神经病理性疼痛 /
- 糖皮质激素受体激动剂 /
- 胶质纤维酸性蛋白 /
- 炎性因子
Abstract: Objective To investigate the effect of glucocorticoid receptor agonists on glial fibrillary acidic protein (GFAP) and inflammatory factors expression in spinal cord of neuropathic pain rats. Methods Twenty SD rats were randomly divided into control group, sham operation group, model group and treatment group. The model group and treatment group were used to establish neuropathic pain model. In the sham operated group, only the sciatic nerve was exposed, but not ligated. Dexamethasone was injected intrathecally in the treatment group, normal saline was injected intrathecally in other three groups for 7 days. The mechanical pain threshold was measured by Von Frey filament one day before operation, three days after operation and seven days after operation. The rats were killed 7 days after operation. The expression of GFAP protein was detected by western blot and the expression of inflammatory factor was detected by RT-PCR. Results The mechanical pain threshold and thermal pain threshold in the treatment group were significantly lower than those in the control group and the sham operation group (P < 0.05), but significantly higher than those in the model group (P < 0.05). The expression level of GFAP protein in the model group and the treatment group was significantly higher than that in the control group and the sham operation group (P < 0.05), but the expression level of GFAP protein in the treatment group was significantly lower than that in the model group (P < 0.05). The expression of TNF-α and IL-1β mRNA in the spinal cord of the model group and the treatment group was significantly higher than that of the control group and the sham operation group (P < 0.05), but the expression of TNF-α and IL-1β mRNA in the spinal cord of the treatment group was significantly lower than that of the model group (P < 0.05). Conclusion Glucocorticoid receptor agonists can significantly reduce the pain sensitivity of neuropathic pain rats, and its mechanism may be related to the down-regulation of GFAP and the expression of downstream inflammatory factors.
- Neuropathic pain /
- Glucocorticoid receptor agonist /
- Glial fibrillary acidic protein /
- Inflammatory factor

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图 1 Western blotting检测各组脊髓GFAP蛋白表达水平

注：与对照组比较，^aP < 0.05；与假手术组比较，^bP < 0.05；与模型组比较，^cP < 0.05。

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表 1 各组大鼠机械痛阈和热痛阈比较(x ±s)

组别	例数	机械痛阈(g)			热痛阈(s)
组别	例数	术前1 d	术后3 d	术后7 d	术前1 d	术后3 d	术后7 d
对照组	5	25.12±2.42	25.14±1.92	25.18±2.37	14.11±1.58	14.14±1.02	14.09±1.76
假手术组	5	25.26±2.28	22.21±1.71^ab	23.74±2.02^abe	14.05±1.37	12.36±1.32^ab	13.28±1.83^abe
模型组	5	25.24±2.62	17.57±1.98^abc	15.92±1.12^abce	14.29±1.07	9.31±1.38^abc	7.12±1.34^abce
治疗组	5	25.19±2.36	20.94±1.61^abcd	18.97±2.18^abcde	14.22±1.24	10.39±1.27^abcd	9.34±1.25^abcde
F值		0.367	24.609	38.623	0.852	18.773	12.915
P值		0.546	< 0.001	< 0.001	0.359	< 0.001	< 0.001
注：A中1为对照组；2为假手术组；3为模型组；4为治疗组；B中与对照组比较，^aP < 0.05；与假手术组相比，^bP < 0.05；与模型组相比，^cP < 0.05。

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表 2 各组大鼠脊髓炎性因子mRNA表达水平比较

组别	例数	TNF-α mRNA	IL-1β mRNA
对照组	5	1.12±0.36	0.77±1.14
假手术组	5	1.35±0.42	0.98±0.18
模型组	5	4.26±1.08^ab	3.92±1.04^ab
治疗组	5	2.05±0.65^abc	1.73±0.39^abc
F值		4.559	3.872
P值		0.006	0.013
注：与同组术前1 d相比，^aP < 0.05；与对照组相比，^bP < 0.05；与假手术组相比，^cP < 0.05；与模型组相比，^dP < 0.05；与同组术后3 d相比，^eP < 0.05。

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