SOX6在口腔黏液表皮样癌中的表达及其临床意义

郑庆蕊; 周美云; 韩影影; 王倩倩; 徐锦程

doi:10.16766/j.cnki.issn.1674-4152.001821

SOX6在口腔黏液表皮样癌中的表达及其临床意义

doi: 10.16766/j.cnki.issn.1674-4152.001821

蚌埠医学院第一附属医院口腔科，安徽蚌埠 233004

基金项目:

安徽省教育厅自然科学基金资助项目 KJ2016A480

详细信息

通讯作者:
徐锦程，E-mail：xjch9999@163.com

中图分类号: R739.8 R730
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出版历程
- 收稿日期: 2020-10-20
- 网络出版日期: 2022-02-19

Expression of SOX6 in oral mucoepidermoid carcinoma and its clinical significance

Department of Stomatology, the First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233004, China

摘要

摘要: 目的检测转录因子性别决定区Y-box 6(SOX6)在唾液腺黏液表皮样癌组织及癌旁正常组织中的表达情况，并探究其表达的差异与临床病理特征及患者预后的关系。方法采用免疫组化EnVision法检测55例唾液腺黏液表皮样癌及相应癌旁正常组织中SOX6蛋白的表达情况，应用统计学方法分析SOX6的表达情况与唾液腺黏液表皮样癌患者的临床病理特征(性别、年龄、病理分级、TNM分期、淋巴结转移)及其预后的关系。结果黏液表皮样癌组织中SOX6阳性表达率为62.3%(37/55)，其相应癌旁正常组织的阳性表达率为36.4%(20/55)，黏液表皮样癌组织中SOX6阳性表达率明显高于其相应癌旁正常组织(P < 0.05)。在黏液表皮样癌中，SOX6表达阳性率的差异与病理分级和临床分期相关(均P < 0.05)。随着唾液腺黏液表皮样癌患者病理分级的降低、TNM分期的升高，SOX6阳性表达率明显升高。但患者性别、年龄与SOX6阳性表达率的高低间的差异无统计学意义(均P>0.05)。统计分析结果示，SOX6阴性组的5年生存期较阳性组长，差异有统计学意义，SOX6的高表达与患者不良预后有明显的相关性(P < 0.05)，SOX6可能是影响唾液腺黏液表皮样癌患者的独立预后因素。结论 SOX6在唾液腺黏液表皮样癌组织中呈高表达，其可能在唾液腺黏液表皮样癌发生、发展过程中扮演着重要角色，这为唾液腺黏液表皮样癌的治疗及预后评估提供一定的参考依据。
- 黏液表皮样癌 /
- 转录因子性别决定区Y-box 6 /
- 免疫组织化学 /
- 预后
Abstract: Objective To detect the expression of transcription factor sex-determining region Y-box 6 (SOX6) in mucoepidermoid carcinoma (MEC) of salivary gland and adjacent normal tissues, as well as to explore the relationship between the expression difference and clinicopathological features and prognosis of patients. Methods The immunohistochemical EnVision method was used to detect the expression of SOX6 protein in 55 cases of salivary gland mucoepidermoid carcinoma and the corresponding normal tissues adjacent to the cancer. The relationship between the expression of SOX6 and the clinicopathological characteristics (gender, age, pathological grade, TNM staging, and lymph-node metastasis) and prognosis of salivary gland mucoepidermoid carcinoma was analysed by statistical methods. Results The positive expression rate of SOX6 in mucoepidermoid carcinoma was 62.3% (37/55), and the positive expression rate of SOX6 in the corresponding normal tissues adjacent to the cancer was 36.4% (20/55). The positive expression rate of SOX6 in mucoepidermoid carcinoma was significantly higher than that of the corresponding normal tissues (P < 0.05). The difference of SOX6 expression in mucoepidermoid carcinoma was related to pathological grade and clinical stage (all P < 0.05). With decreased pathological grade of MEC patients and increased TNM stage, the expression of SOX6 increased. But there was no statistical significance between gender, age and SOX6 positive expression rate (all P>0.05). The results of statistical analysis showed that the five-year survival time of the SOX6 negative group was longer than that of the positive group, and the difference was statistically significant. The high expression of SOX6 was significantly correlated with the poor prognosis of patients (P < 0.05). SOX6 may be an independent prognostic factor in patients with mucoepidermoid carcinoma of salivary gland. Conclusion SOX6 is highly expressed in MEC tissues and may play an important role in the occurrence of MEC, which has certain reference value for the treatment and diagnosis of MEC.
- Mucoepidermoid carcinoma /
- Transcription factor sex determining region Y-box 6 /
- Immunohistochemistry /
- Prognosis

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图 1 SOX6在MEC和癌旁正常上皮的表达(EnVision法, ×400)

注：A为SOX6在癌旁正常上皮组织中的表达；B为SOX6在MEC组织中的表达

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图 2 SOX6对MEC患者5年生存期的影响

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表 1 MEC中SOX6的表达与临床病理参数的关系 [例(%)]

临床特征		例数	SOX6表达		χ²值	P值
临床特征		例数	-	+	χ²值	P值
性别	男性	27	10(37.0)	17(63.0)	0.447	0.504
	女性	28	8(28.6)	20(71.4)
年龄	≤45岁	29	9(31.0)	20(69.0)	0.080	0.778
	>45岁	26	9(34.6)	17(65.4)
淋巴结转移	无	33	14(42.4)	19(57.6)	3.524	0.061
	有	22	4(18.2)	18(81.8)
病理分级	高分化	25	12(48.0)	13(52.0)	4.856	0.028
	低/中分化	30	6(20.0)	24(80.0)
TNM分期	Ⅰ+Ⅱ期	20	10(50.0)	10(50.0)	4.259	0.039
	Ⅲ+Ⅳ期	35	8(22.9)	27(77.1)

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表 2 影响MEC患者5年生存率的COX单因素回归分析

因素	B	SE	Wald χ²	P值	HR(95% CI)
性别	0.147	0.45	0.107	0.743	1.159(0.480~2.796)
年龄	-0.355	0.456	0.603	0.437	0.701(0.287~1.716)
淋巴结转移	0.237	0.45	0.277	0.599	1.267(0.525~3.061)
病理分级	1.051	0.517	4.128	0.042	2.860(1.038~7.882)
TNM分期	1.284	0.627	4.186	0.041	3.610(1.055~12.348)
SOX6表达	1.801	0.747	5.816	0.016	6.056(1.401~26.176)

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表 3 影响MEC患者5年生存率的COX多因素回归分析

因素	B	SE	Wald χ²	P值	HR(95% CI)
性别	-0.223	0.482	0.215	0.643	0.800(0.311~2.056)
年龄	-0.097	0.502	0.037	0.847	0.908(0.339~2.427)
淋巴结转移	-0.658	0.511	1.661	0.198	0.518(0.19~1.409)
病理分级	0.421	0.589	0.512	0.474	1.524(0.481~4.828)
TNM分期	1.184	0.724	2.676	0.102	3.269(0.791~13.511)
SOX6表达	1.591	0.777	4.195	0.041	4.907(1.071~22.483)

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