木犀草素对人肺癌细胞株H460增殖和迁移的影响

储娜; 张璇; 阮大鹏; 陈思远; 王允

doi:10.16766/j.cnki.issn.1674-4152.001904

木犀草素对人肺癌细胞株H460增殖和迁移的影响

doi: 10.16766/j.cnki.issn.1674-4152.001904

蚌埠医学院公共卫生学院，安徽蚌埠 233000

基金项目:

安徽省高校自然科学研究项目 KJ2019A0316

蚌埠医学院研究生科研创新项目 Byycx1907

蚌埠医学院研究生科研创新项目 Byycx20051

详细信息

通讯作者:
王允，E-mail：wy_sunnyday@126.com

中图分类号: R734.2 R730.43
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- 文章访问数: 267
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- 被引次数: 0
出版历程
- 收稿日期: 2020-11-26
- 网络出版日期: 2022-02-16

Effect of luteolin on proliferation and migration of human lung cancer cell line H460

School of Public Health, Bengbu Medical College, Bengbu, Anhui 233000, China

摘要

摘要: 目的探讨木犀草素(luteolin，LUT)对人肺大细胞癌H460细胞增殖和迁移能力的影响，并初步分析其作用机制。方法不同浓度的LUT处理H460细胞24 h或48 h后，用MTT法检测细胞活性；用平板克隆形成实验和划痕实验分别检测其克隆形成能力和迁移能力；用蛋白免疫印迹法检测上皮性钙粘附素(E-cadherin)和神经性钙粘附素(N-cadherin)蛋白的表达水平。结果 LUT可呈浓度和时间依赖性抑制H460细胞的活性(P＜0.05)。0、10和20 μmol/L LUT处理H460细胞48 h后，Control、LUT10和LUT20处理组的克隆形成率分别为(1.00±0.05)%、(0.78±0.05)%和(0.49±0.04)%，划痕愈合度分别为(30.37±2.92)%、(26.23±2.42)%和(22.16±1.74)%，N-cadherin和E-cadherin蛋白的表达水平分别为0.62±0.01、0.61±0.01、0.59±0.01，0.85±0.05、0.88±0.05、0.94±0.05。即与Control组相比，LUT10和LUT20处理组的平板克隆形成率和划痕愈合度均下降(均P＜0.05)，E-cadherin蛋白表达水平上调(均P＜0.05)，N-cadherin蛋白表达水平下调(均P＜0.05)。结论 LUT可有效抑制H460细胞的增殖和迁移，这可能与其对E-cadherin和N-cadherin等上皮间质转化相关标志蛋白的调控有关。
- 木犀草素 /
- H460细胞 /
- 上皮间质转化 /
- 化学预防
Abstract: Objective To explore the effect of luteolin (LUT) on the proliferation and migration of human lung large cell carcinoma H460 cells and analyse its mechanism of action. Methods After the H460 cells were treated with different concentrations of LUT for 24 h or 48 h, the cell viability was detected by MTT assay. The clone formation ability and migration ability were detected by plate clone formation test and scratch test, respectively. The expression levels of E-cadherin and N-cadherin protein were detected by Western blotting. Results LUT can inhibit the activity of H460 cells in a concentration-dependent and time-dependent manner (P < 0.05). After H460 cells were treated with 0, 10 and 20 μmol/L LUT for 48 h, the clone formation rates of the control, LUT10 and LUT20 groups respectively were 1.00±0.05, 0.78±0.05 and 0.49±0.04, and the scratch healing degree respectively were (30.37 ±2.92)%, (26.23±2.42)% and (22.16 ±1.74)%. The expression levels of N-cadherin and E-cadherin proteins were 0.62±0.01, 0.61±0.01 and 0.59±0.01; 0.85±0.05, 0.88 ±0.05 and 0.94 ±0.05, respectively. Compared with those of the control group, the plate clone formation rate and scratch healing rate of LUT10 and LUT20 groups decreased (all P < 0.05), the expression level of E-cadherin protein was up-regulated (all P < 0.05) and the expression level of N-cadherin protein was down-regulated (all P < 0.05). Conclusion LUT can effectively inhibit the proliferation and migration of H460 cells, which may be related to the regulation of E-cadherin and N-cadherin-related marker proteins of epithelial-mesenchymal transition.
- Luteolin /
- H460 cell line /
- Epithelial to mesenchymal transition /
- Chemoprevention

HTML全文

图 1 LUT对H460细胞活性的影响

注：与0 μmol/L LUT处理组比较, ^aP＜0.05。

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图 2 LUT对H460细胞克隆形成能力的影响

注：与对照组比较, ^aP＜0.05。

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图 3 LUT对H460细胞迁移能力的影响

注：与对照组比较, ^aP＜0.05。

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图 4 LUT对H460细胞相关蛋白表达的影响

注：与对照组比较, ^aP＜0.05。

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