HYAL1表达与头颈鳞状细胞癌进展和预后的关系

苏奎玮; 李枭; 程刚

doi:10.16766/j.cnki.issn.1674-4152.001909

HYAL1表达与头颈鳞状细胞癌进展和预后的关系

doi: 10.16766/j.cnki.issn.1674-4152.001909

苏奎玮¹,
李枭¹,
程刚^{1, 2, ,}

1.
蚌埠医学院研究生院，安徽蚌埠 233030
2.
浙江省人民医院口腔科，浙江杭州 310014

基金项目:

安徽省教育厅自然科学基金资助项目 KJ2016A480

详细信息

通讯作者:
程刚, E-mail: gang_cheng001@hotmail.com

中图分类号: R739.91 R730.261
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- 文章访问数: 158
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- PDF下载量: 1
- 被引次数: 0
出版历程
- 收稿日期: 2020-09-21
- 网络出版日期: 2022-02-16

The relationship between the expression of HYAL1 and the progression and prognosis of head and neck squamous cell carcinoma

SU Kui-wei¹,
LI Xiao¹,
CHENG Gang^{1, 2
, ,}

1.
Graduate School of Bengbu Medical College, Bengbu, Anhui 233030, China

摘要

摘要: 目的本文旨在检查透明质酸酶1(HYAL1)在头颈鳞状细胞癌(HNSCC)中的表达水平及其与头颈鳞状细胞癌(HNSCC)患者的生存关系。方法收集2008—2015年浙江省人民医院行根治性切除的HNSCC患者资料构建组织芯片，通过免疫组织化学检测了258例患者切除的肿瘤标本和附近组织中HYAL1的表达。结果 HYAL1在HNSCC组织中的表达明显低于正常邻近组织(P < 0.001)。HYAL1的低表达与肿瘤大小(P=0.011)、淋巴结转移(P=0.025)和TMN分期(P=0.038)显著相关。此外，与高HYAL1表达者相比，低HYAL1表达者与短生存期显著相关(P=0.007)。HYAL1低表达患者的总生存期中位数为36个月，而HYAL1高表达患者的总生存期仍未达到中位数(超过74个月)。在本研究有限的病例中，未发现高表达或低表达HYAL1的患者在年龄、性别、病变部位、HPV感染、吸烟、饮酒、组织学分期有任何差异。结论 HYAL1低表达与HNSCC患者的预后不良有关。HYAL1可能是临床上有价值的预后指标，并且是HNSCC治疗的潜在靶标。
- 头颈鳞状细胞癌 /
- 透明质酸酶1 /
- 生物标志物
Abstract: Objective This article aims to study the expression level of hyaluronidase 1 (HYAL1) in head and neck squamous cell carcinoma (HNSCC) and its relationship with the survival of HNSCC patients. Methods The data of HNSCC patients who underwent radical resection in Zhejiang Provincial People's Hospital from 2008 to 2015 were collected to construct tissue chips, and the expression of HYAL1 in tumor specimens and nearby tissues from 258 patients was detected by immunohistochemistry. Results The expression of HYAL1 in HNSCC tissues was significantly lower than that of normal adjacent tissues (P < 0.001). The low expression of HYAL1 was significantly correlated with tumor size (P=0.011), lymph node metastasis (P=0.025) and TNMs staging system (P=0.038). In addition, compared with high HYAL1 expression, low HYAL1 expression was significantly associated with short survival time (P=0.007). The median overall survival of patients with low HYAL1 expression is 36 months, while the median overall survival of patients with high HYAL1 expression has not yet reached (more than 74 months). It is worth noting that in our limited cases, this study did not find any difference in age, gender, lesion site, HPV infection, smoking, drinking, and histological staging between the patients with high low expression of HYAL1. Conclusion The low expression of HYAL1 is related to the poor prognosis of these HNSCC patients. HYAL1 may be a clinically valuable prognostic indicator and a potential target for HNSCC treatment.
- Head and neck squamous cell carcinoma /
- Hyaluronidase 1 /
- Biomarker

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图 1 HYAL1的表达与HNSCC患者生存时间的关系

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表 1 HYAL1在头颈鳞状细胞癌中过表达(例)

组织类型	总计	HYAL1评分		χ²值	P值
组织类型	总计	高	低	χ²值	P值
头颈鳞状细胞癌	258	156	102	22.574	< 0.001
癌旁组织	132	111	21

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表 2 HYAL1的表达与头颈鳞状细胞癌临床特征之间的关系(例)

项目	例数	HYAL1表达水平		χ²值	P值
项目	例数	高	低	χ²值	P值
性别				0.492	0.483
男性	203	125	78
女性	55	31	24
年龄(岁)				0.001	0.978
< 60	66	40	26
≥60	192	116	76
肿瘤大小(cm)				9.531	0.002
≤5	173	116	57
> 5	85	40	45
病变部位				5.847	0.664
牙龈	7	3	4
舌根	23	11	12
颊黏膜	36	16	20
口底	11	4	7
硬腭	14	7	7
咽部	8	4	3
唇	28	12	16
舌	36	10	26
喉	14	8	6
HPV感染				2.393	0.122
阳性	116	69	47
阴性	71	34	37
淋巴结转移				6.757	0.009
否	212	136	76
是	46	20	26
吸烟				0.648	0.421
是	153	88	65
否	64	33	31
TMN分期				8.439	0.004
Ⅰ~Ⅱ	201	131	70
Ⅲ~Ⅳ	57	25	32
组织学分期				4.206	0.122
高	53	21	32
中	132	72	60
低	25	15	10
饮酒				1.622	0.203
否	132	76	56
是	94	62	32

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表 3 HNSCC患者临床病理参数和HYAL1表达的单因素Cox回归生存分析

项目	B	SE	Wald χ²	P值	HR(95% CI)
HYAL1表达	1.166	0.681	7.472	0.009	0.574(0.329~0.902)
肿瘤大小	0.691	0.247	7.831	0.005	1.995(1.230~3.236)
TMN分期	0.609	0.269	5.143	0.023	1.839(1.086~3.115)
组织学分期	0.345	0.309	1.246	0.017	2.134(1.260~3.628)
淋巴结转移	1.087	0.762	12.847	0.001	2.438(1.692~4.446)
HPV感染	0.087	0.061	2.046	0.153	1.091(0.969~1.226)

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表 4 HNSCC患者临床病理参数和HYAL1表达的多因素Cox回归生存分析

项目	B	SE	Wald χ²	P值	HR(95% CI)
HYAL1表达	0.509	0.241	4.454	0.035	0.601(0.375~0.964)
肿瘤大小	0.337	0.212	2.515	0.113	1.401(0.924~2.124)
TMN分期	1.595	0.326	23.970	< 0.001	4.929(2.603~9.335)
组织学分期	0.830	0.280	8.794	0.003	2.294(1.325~3.972)
淋巴结转移	1.053	0.247	18.225	< 0.001	2.868(1.768~3.651)
HPV感染	0.097	0.242	0.161	0.689	1.102(0.685~1.772)

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