溃疡性结肠炎患者中TLR9和STAT3的表达及其与IL-10的相关性研究

张雯婷; 顾林; 柯希权; 王启之; 汪强武; 汪建超; 李大鹏

doi:10.16766/j.cnki.issn.1674-4152.001950

溃疡性结肠炎患者中TLR9和STAT3的表达及其与IL-10的相关性研究

doi: 10.16766/j.cnki.issn.1674-4152.001950

蚌埠医学院第一附属医院消化内科，安徽蚌埠 233004

基金项目:

安徽省教育厅2018年度高校优秀拔尖人才培育资助项目 gxgwfx2018052

蚌埠医学院自然科学重点项目 BYKY2019085ZD

详细信息

通讯作者:
王启之，E-mail：wangqz2004@sina.com

中图分类号: R574.62 R446
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- 文章访问数: 231
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- 被引次数: 0
出版历程
- 收稿日期: 2020-10-22
- 网络出版日期: 2022-02-16

Expressions of TLR9 and STAT3 and their correlation with IL-10 in patients with ulcerative colitis

Department of Gastroenterology, the First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233004, China

摘要

摘要: 目的检测Toll样受体9(TLR9)、信号转导与转录活化因子3(STAT3)及白介素10(IL-10)在溃疡性结肠炎(ulcerative colitis，UC)患者外周血及肠黏膜组织中的表达水平, 探讨它们在UC发病中的作用及其相互关系。方法选取2019年7月—2020年7月在蚌埠医学院第一附属医院诊治的32例UC患者归为实验组，30例健康体检者纳入正常对照组，其中实验组又分为轻度(10例)、中度(14例)及重度组(8例)。检测外周血单个核细胞中TLR9 mRNA和STAT3 mRNA的表达和血清IL-10水平及肠黏膜组织中TLR9、STAT3及IL-10蛋白表达水平。结果实验组UC患者TLR9 mRNA和STAT3 mRNA的表达水平均高于正常对照组(均P < 0.05)；血清中IL-10的表达水平明显低于正常对照组(P < 0.05)。肠黏膜组织中TLR9、STAT3的蛋白表达水平均高于正常对照组(均P < 0.05)，IL-10蛋白表达水平低于正常对照组(P < 0.05)。TLR9 mRNA和STAT3 mRNA、TLR9和STAT3蛋白表达水平之间均呈正相关，血清IL-10与TLR9 mRNA、STAT3 mRNA表达水平呈负相关，肠黏膜组织IL-10与TLR9、STAT3蛋白表达水平呈负相关。结论 TLR9、STAT3、IL-10共同参与了UC的发生和发展，在UC的发病机制中TLR9和STAT3可能参与了IL-10的表达与调控。
- 溃疡性结肠炎 /
- Toll样受体9 /
- 信号转导与转录活化因子3 /
- 白介素10
Abstract: Objective The expression levels of toll-like receptor 9 (TLR9), signal transduction and transcriptional activator factor 3 (STAT3) and interleukin-10 (IL-10) in peripheral blood and intestinal mucosal tissues of patients with ulcerative colitis (UC) were detected to explore their roles in the pathogenesis of UC and their correlation. Methods Thirty-two patients with UC treated in the First Affiliated Hospital of Bengbu Medical College from July 2019 to July 2020 were selected as the experimental group; and 30 healthy subjects according to the results of physical examination were included in the normal control group. The experimental group was further divided into mild group (n=10), moderate group (n=14) and severe (n=8) group. The expressions of TLR9 and STAT3 mRNA in peripheral blood mononuclear cells, serum IL-10 levels and TLR9, as well as levels of STAT3 and IL-10 proteins, in intestinal mucosal tissues were detected. Results The expression levels of TLR9 mRNA and STAT3 mRNA in the experimental group were both higher than those in the control group (all P < 0.05). The expression level of IL-10 in serum in the experimental group was significantly lower than that in the normal control group (P < 0.05). The expression levels of TLR9 and STAT3 in intestinal mucosal tissues were all higher than that in the normal control group (all P < 0.05). By contrast, the expression levels of IL-10 in the experimental group were lower than that in the normal control group (P < 0.05). Expressions of TLR9 mRNA and STAT3 mRNA and TLR9 and STAT3 mRNA were positively correlated. Serum IL-10 was negatively correlated with TLR9 and STAT3 mRNA expression levels. Intestinal mucosal IL-10 was negatively correlated with TLR9 and STAT3 protein expression levels. Conclusion TLR9, STAT3 and IL-10 are jointly involved in the occurrence and development of UC. TLR9 and STAT3 may be involved in the expression and regulation of IL-10 in the pathogenesis of UC.
- Ulcerative colitis /
- Toll like receptors 9 /
- STAT3 /
- interleukin-10

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图 1 免疫组织化学检测相关蛋白表达(×400)

注：横向依次为TLR9、STAT3和IL-10蛋白表达；纵向依次为对照组、轻度组、中度组、重度组。

下载: 全尺寸图片幻灯片

表 1 RT-PCR引物系列及产物长度一览表

基因	引物方向	引物序列(5' -3')	扩增产物长度(bp)
TLR9	上游引物	ACTGGCTGTTCCTGAAGTCTGTG	254
	下游引物	CAGGTTTAGCTCTTCCAGGGTG
STAT3	上游引物	GAGAAGGACATCAGCGGTAAG	138
	下游引物	CAGTGGAGACACCAGGATATTG
β-actin	上游引物	GGAAGCTTGTCATCAATGGAAATC	168
	下游引物	TGATGACCCTTTTGGCTCCC

下载: 导出CSV

表 2 各组TLR9 mRNA、STAT3 mRNA水平及IL-10表达 (x ±s，ng/mL)

组别	分级	例数	TLR9	STAT3	IL-10
对照组		30	0.80±0.12	0.83±0.13	56.56±7.11
实验组	轻度	10	0.98±0.05^a	1.23±0.06^a	44.61±2.53^a
	中度	14	1.15±0.09^ab	1.31±0.13^a	35.54±6.09^ab
	重度	8	1.56±0.28^abc	1.82±0.14^abc	28.78±3.37^abc
F值			70.183	145.237	67.395
P值			< 0.001	< 0.001	< 0.001
注：与对照组比较, ^aP < 0.05；与轻度组比较, ^bP < 0.05；与中度组比较，^cP < 0.05。

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表 3 各组TLR9、STAT3及IL-10蛋白表达水平的平均吸光度值 (x ±s，ng/mL)

组别	分级	例数	TLR9	STAT3	IL-10
对照组		30	0.28±0.09	0.23±0.12	1.98±0.19
实验组	轻度	10	0.60±0.10^a	0.81±0.22^a	1.36±0.05^a
	中度	14	0.95±0.09^ab	1.08±0.09^ab	0.88±0.14^ab
	重度	8	1.73±0.23^abc	2.07±0.18^abc	0.60±0.11^abc
F值			351.765	377.283	268.492
P值			< 0.001	< 0.001	< 0.001
注：与对照组比较, ^aP < 0.05；与轻度组比较, ^bP < 0.05；与中度组比较，^cP < 0.05。

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表 4 TLR9 mRNA、STAT3 mRNA及IL-10之间的相关性

指标	r值	P值
TLR9/STAT3	0.809	< 0.001
TLR9/IL-10	-0.749	< 0.001
STAT3/IL-10	-0.838	< 0.001

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表 5 TLR9、STAT3及IL-10蛋白表达水平之间的相关性

指标	r值	P值
TLR9/STAT3	0.940	< 0.001
TLR9/IL-10	-0.892	< 0.001
STAT3/IL-10	-0.898	< 0.001

下载: 导出CSV

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