Mechanism of San'ao Decoction in treatment of cough variant asthma on the basis of network pharmacology
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摘要:
目的 根据网络药理学方法对三拗汤治疗咳嗽变异型哮喘的作用机制进行探讨。 方法 利用中药系统药理学数据库与分析平台(TCMSP)对三拗汤中有效成分进行筛选及靶点预测，检索GeneCards、OMIM数据库筛选CVA疾病靶点，借助网络拓扑分析插件CytoNCA筛选三拗汤治疗CVA核心靶点，使用Cytoscape 3.7.1建立化合物-疾病-靶点调控网络，基于DAVID数据库对核心靶点进行GO功能富集和KEGG通路富集分析。 结果 基于TCMSP得到三拗汤的114个有效成分和254个潜在靶点，筛选获得与CVA疾病发生、发展相关的靶点2 257个，借助网络拓扑分析插件CytoNCA筛选得到三拗汤治疗CVA的核心靶点28个，GO功能富集分析得到条目328个，其中生物过程条目264个、分子功能条目46个、细胞组成条目18个，主要包括细胞凋亡、脂多糖反应、丝裂原活化蛋白激酶(MAPK)活性、一氧化氮合酶活性等功能途径；KEGG通路富集分析得出条目115个，主要包括MAPK信号通路、肿瘤坏死因子信号通路、Th17细胞分化等通路途径。 结论 基于网络药理学初步探讨并验证了三拗汤治疗CVA多成分、多靶点、多通路的整体调节作用特点，预测了三拗汤治疗CVA的潜在作用机制，以期为其活性成分研究与实验研究提供科学依据。 Abstract:Objective To explore the mechanism of San'ao Decoction in the treatment of cough variant asthma (CVA). Methods The TCMSP database was used to screen the effective components and predict the target of San'ao Decoction. GeneCards and OMIM database were searched to screen the CVA disease target. CytoNCA was used to screen the core target of San'ao Decoction in the treatment of CVA. Cytoscape 3.7.1 was used to establish a compound disease target regulatory network. On the basis of DAVID database, GO and KEGG pathway enrichment analyses were conducted to determine the core targets of San'ao Decoction. Results On the basis of TCMSP, 114 effective components and 254 potential targets of San'ao Decoction were obtained. A total of 2 257 targets related to the occurrence and development of CVA were screened. Twenty-eight core targets of San'ao Decoction for CVA treatment were screened by using the network topology analysis plug-in CytoNCA. Total 328 entries were obtained by GO function enrichment analysis, including 264 entries of biological process and 46 entries of molecular function and 18 orders of cell composition, mainly including apoptosis, lipopolysaccharide reaction, mitogen activated protein kinase (MAPK) activity, nitric oxide synthetase activity and other functional pathways. A total of 115 entries were obtained from KEGG pathway enrichment analysis, mainly including MAPK signalling pathway, tumour necrosis factor signalling pathway, Th17 cell differentiation and other pathways. Conclusion On the basis of network pharmacology, the overall regulatory characteristics of San'ao Decoction in the treatment of CVA, including multi-component, multi-target and multi-channel, are preliminarily discussed and verified, and the potential mechanism of San'ao Decoction in the treatment of CVA is predicted so as to provide scientific basis for the research of its active components. -
Key words:
- Network pharmacology /
- San'ao Decoction /
- Cough variant asthma /
- Signalling pathway /
- Target
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表 1 数据库及分析平台
名称 网址 版本 中药系统药理学数据库与分析平台 http://tcmspw.com/tcmsp.php 2.3 UniProt数据库 https://www.uniprot.org GeneCards人类基因数据库 https://www.genecards.org 4.12 OMIM人类孟德尔遗传数据库 https://www.omim.org 1.1 STRING数据库在线平台 https://string-db.org/cgi/input.pl 11.0 DAVID数据库 https://david.ncifcrf.gov/home.jsp 6.8 韦恩图在线平台 https://bioinfogp.cnb.csic.es/tools/venny/index.html 2.1.0 Cytoscape https://cytoscape.org 3.7.1 
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[1] 徐扬扬. 呼吸内科门诊180例慢性咳嗽病因以及影响因素分析[J]. 中西医结合心血管病电子杂志, 2020, 8(10): 51. https://www.cnki.com.cn/Article/CJFDTOTAL-ZXJH202010043.htm [2] 中华医学会呼吸病学分会哮喘学组. 咳嗽的诊断与治疗指南(2015)[J]. 中华结核和呼吸杂志, 2016, 39(5): 323-354. doi: 10.3760/cma.j.issn.1001-0939.2016.05.003 [3] 安鹏. 三拗汤治疗咳嗽变异性哮喘的临床疗效评价[J]. 世界最新医学信息文摘, 2017, 17(92): 104. https://www.cnki.com.cn/Article/CJFDTOTAL-WMIA201792086.htm [4] 黄惠芬, 范良, 傅汝梅, 等. 玄参甘桔汤合三拗汤加减治疗燥热伤肺型感染后咳嗽的疗效观察[J]. 中国实验方剂学杂志, 2019, 25(3): 125-130. https://www.cnki.com.cn/Article/CJFDTOTAL-ZSFX201903020.htm [5] 孟凡翠, 汤立达. 中药网络药理学研究中存在的问题与发展展望[J]. 中草药, 2020, 51(8): 2232-2237. https://www.cnki.com.cn/Article/CJFDTOTAL-ZCYO202008034.htm [6] 邢心睿, 吕狄亚, 柴逸峰, 等. 网络药理学在中药作用机制中的研究进展[J]. 药学实践杂志, 2018, 36(2): 97-102. doi: 10.3969/j.issn.1006-0111.2018.02.001 [7] 高龙霞, 阎玥, 包海鹏. 咳嗽变异性哮喘现代研究进展[J], 中华中医药杂志, 2019, 34(9): 4171-4174. https://www.cnki.com.cn/Article/CJFDTOTAL-BXYY201909072.htm [8] 李淑玲, 马春, 杨丽华. 中医药治疗CVA的研究进展[J]. 中国中医药现代远程教育, 2017, 15(23): 153-155. doi: 10.3969/j.issn.1672-2779.2017.23.068 [9] 李德斌. 三拗汤加减治疗咳嗽变异性哮喘40例临床观察[J]. 医学食疗与健康, 2019(18): 44-46. https://www.cnki.com.cn/Article/CJFDTOTAL-YXSL201918030.htm [10] 王雨墨, 吴丽霞, 张弘, 等. 王坤根教授应用祛风法治疗咳嗽变异性哮喘的经验[J]. 陕西中医药大学学报, 2017, 40(2): 24-26. https://www.cnki.com.cn/Article/CJFDTOTAL-SXXY201702008.htm [11] 陈海彬, 周红光, 李文婷, 等. 网络药理学-中药复方作用机制研究新视角[J]. 中华中医药杂志, 2019, 34(7): 2873-2876. https://www.cnki.com.cn/Article/CJFDTOTAL-BXYY201907009.htm [12] 沈凤, 王明慧. 槐杞黄颗粒联合酮替芬和孟鲁司特对小儿咳嗽变异性哮喘患者免疫功能的影响[J]. 中国临床研究, 2019, 32(2): 256-259. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGCK201902027.htm [13] 徐超极. 基于Th1/Th2失衡机制观察儿童咳嗽变异性哮喘中医分期治疗的临床疗效[D]. 福州: 福建中医药大学, 2018. [14] 邹华, 石立慧, 王颖, 等. 木犀草素通过诱导型一氧化氮合酶/一氧化氮途径抑制哮喘幼鼠气道炎症[J]. 解剖科学进展, 2020, 26(2): 146-149. https://www.cnki.com.cn/Article/CJFDTOTAL-JPKX202002008.htm [15] 吴守业, 张余转. 支气管哮喘患儿诱导痰嗜酸粒细胞与肺功能及FeNO浓度的关系[J]. 国际呼吸杂志, 2020, 40(5): 355-356, 358. [16] 汪夙兴. 呼出气一氧化氮水平与诱导痰嗜酸性粒细胞联合检测对哮喘-慢性阻塞性肺疾病重叠综合征、哮喘、慢性阻塞性肺疾病患者的诊断价值[J]. 中国当代医药, 2020, 27(5): 12-15. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGUD202005004.htm [17] 高龙霞, 阎玥, 包海鹏, 等. 咳嗽变异性哮喘现代研究进展[J]. 中华中医药杂志, 2019, 34(9): 4171-4174. https://www.cnki.com.cn/Article/CJFDTOTAL-BXYY201909072.htm [18] 窦晓宾, 吴铁峰, 蔡振荡. 效应T细胞、调节T细胞失衡与支气管哮喘患儿病情程度的相关性及对疾病控制情况的预测价值[J]. 中华全科医学, 2019, 17(4): 597-600. https://www.cnki.com.cn/Article/CJFDTOTAL-SYQY201904023.htm [19] 陈存国, 金华良. 老年哮喘患者呼出气一氧化氮水平与病情严重程度和治疗效果的关系[J]. 中华全科医学, 2017, 15(1): 33-35. https://www.cnki.com.cn/Article/CJFDTOTAL-SYQY201701011.htm [20] 徐凤, 肖韩艳, 周淑芬, 等. 柴朴汤对哮喘模型大鼠气道炎症及ERK/p38 MAPK信号通路的影响[J]. 中国实验方剂学杂志, 2018, 24(2): 104-109. https://www.cnki.com.cn/Article/CJFDTOTAL-ZSFX201802019.htm [21] 康建强, 董杨阳, 杨玲, 等. 下呼吸道流感嗜血杆菌定植对哮喘小鼠气道炎症的影响及信号通路的研究[J]. 诊断学理论与实践, 2020, 19(1): 44-49. https://www.cnki.com.cn/Article/CJFDTOTAL-ZDLS202001014.htm [22] 黄羽, 刘瑛. 孟鲁司特对咳嗽变异性哮喘患儿Treg及Th17相关细胞因子表达的影响[J]. 昆明医科大学学报, 2019, 40(6): 125-128. https://www.cnki.com.cn/Article/CJFDTOTAL-KMYX201906026.htm [23] 孙婧怡, 马礼兵. 咳嗽变异性哮喘的发病机制及研究进展[J]. 实用医学杂志, 2018, 34(16): 2803-2806. https://www.cnki.com.cn/Article/CJFDTOTAL-SYYZ201816043.htm -
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