精神分裂症患者肠道菌群与临床症状的相关性研究

王中娴; 张爱国; 姚理慧; 程夏龙; 程鹏; 朱翠珍; 苏虹; 张许来

doi:10.16766/j.cnki.issn.1674-4152.002104

精神分裂症患者肠道菌群与临床症状的相关性研究

doi: 10.16766/j.cnki.issn.1674-4152.002104

王中娴^{1, 2},
张爱国^{1, 2},
姚理慧^{1, 2},
程夏龙^{1, 2},
程鹏^{1, 2},
朱翠珍^{1, 2},
苏虹³,
张许来^{1, 2, ,}

1.
安徽省精神卫生中心，安徽合肥 230022
2.
合肥市第四人民医院精神科，安徽省精神心理疾病临床研究中心，安徽合肥230022
3.
安徽医科大学公共卫生学院流行病与卫生统计学系，安徽合肥 230032

基金项目:

国家自然科学基金项目 81773518

合肥市科技局“借转补”项目 J2018Y05

详细信息

通讯作者:
张许来, E-mail：Xulaizhang@ahmhcentre.com

中图分类号: R749.3 R57
计量
- 文章访问数: 155
- HTML全文浏览量: 119
- PDF下载量: 5
- 被引次数: 0
出版历程
- 收稿日期: 2020-06-15
- 网络出版日期: 2022-02-15

Correlation between intestinal flora and clinical symptoms in patients with schizophrenia

WANG Zhong-xian^{1, 2},
ZHANG Ai-guo^{1, 2},
YAO Li-hui^{1, 2},
CHENG Xia-long^{1, 2},
CHENG Peng^{1, 2},
ZHU Cui-zhen^{1, 2},
SU Hong³,
ZHANG Xu-lai^{1, 2
, ,}

1.
Anhui Metal Health Center, Hefei, Anhui 230022, China

摘要

摘要: 目的探究精神分裂症菌群结构特征与临床症状的相关性。方法纳入2018年10月—2019年6月合肥市第四人民医院收治的精神分裂症患者急性发作期(A组)32例, 缓解期(B组)30例，健康对照(C组)34人。运用16SrRNA技术分析菌群差异及与临床症状的相关性。结果 (1) 另枝菌属A组丰度高于C组(F=2.575，P=0.017)，硫杆菌属A组丰度低于C组(F=3.940，P=0.023)；巨球型菌属A组、B组丰度高于C组(F=11.720，P < 0.001)，毛螺菌属、粪球菌属A组、B组丰度低于C组(F=4.279、3.670，均P < 0.05)；拟杆菌属、嗜血杆菌属、酪酸梭菌属A组丰度高于B组(F=2.977、4.840、2.655，均P < 0.05)，琥珀酸弧菌属A组丰度低于B组(F=6.190，P=0.003)。(2)小杆菌属与抑郁因子呈负相关(r=-0.269，P=0.034)；巨单胞菌属与阳性和阴性症状量表(PANSS)总分呈正相关(r=0.291，P=0.022)；拟杆菌属与阳性因子成正相关(r=0.282，P=0.029)。结论精神分裂症急性发作期和缓解期菌群结构改变，且部分菌群相对丰度与临床症状相关。
- 精神分裂症 /
- 肠道菌群 /
- 高通量测序 /
- 临床症状
Abstract: Objective This study aimed to explore the correlation between the structural characteristics of schizophrenia flora and clinical symptoms. Methods Patients who were diagnosed with schizophrenia in the fourth hospital in Hefei from October 2018 to June 2019 were included in this study: 32 patients in the acute stage (group A), 30 in the remission stage (group B) and 34 in the healthy control group (group C). The 16SrRNA technique was used to analyse the difference of bacterial flora and its correlation with clinical symptoms. Results (1) The abundance of other mycobacteria in group A was higher than that in group C (F=2.575, P=0.017), whereas the abundance of thiobacillus in group A was lower than that in group C (F=3.940, P=0.023). The abundance of macrococcidia in groups A and B was higher than that of group C (F=11.720, P < 0.001), and the abundance of trichoderma and coprococcus in groups A and B was lower than that of group C (F=4.279、3.670, all P < 0.05). The abundance of Bacteroidetes, haemophilus and Clostridium tyrosine in group A was higher than that in group B (F=2.977, 4.840; F=2.655, P < 0.05), and the abundance of vibrio succinate was lower than that of group B (F=6.190, P=0.003). (2) Microbacilli were negatively correlated with depression factors (r=-0.269, P=0.034). Macrosomonas was positively correlated with the total PANSS score (r=0.291, P=0.022). Bacteroidetes was positively correlated with positive factors (r=0.282, P=0.029). Conclusion The structural changes of the microflora in the acute and remission stages of schizophrenia and the relative abundance of some microflora were correlated with clinical symptoms.
- Schizophrenia /
- Intestinal flora /
- High-throughput sequencing /
- Clinical symptoms

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图 1 主坐标分析(PCoA分析)

注：红色为A组，急性发作期；蓝色为B组，缓解期；黄色为C组，健康对照组。

下载: 全尺寸图片幻灯片

图 2 门水平的物种丰度热图

注：A为急性发作期；B为缓解期；C为健康对照组。

下载: 全尺寸图片幻灯片

图 3 属水平的物种丰度热图(log10)

注：A为急性发作期；B为缓解期；C为健康对照组。

下载: 全尺寸图片幻灯片

表 1 精神分裂症患者发作期与缓解期PANSS评分比较(x±s，分)

组别	例数	阳性症状	阴性症状	认知因子	兴奋因子	抑郁因子	总分
A组	32	15.44±4.77	19.44±5.09	10.78±2.14	10.41±2.54	4.28±2.23	71.44±8.20
B组	30	9.03±2.11	13.67±4.40	6.83±1.86	5.70±1.06	3.20±0.48	45.67±6.64
t值		6.764	4.788	7.776	9.417	2.674	13.639
P值		＜0.001	＜0.001	＜0.001	＜0.001	0.011	＜0.001

下载: 导出CSV

表 2 3组肠道菌群的Alpha多样性指数比较(x±s)

组别	例数	chao指数	ace指数	observed指数	simpson指数	shannon指数	coverage指数
A组	32	338.53±73.88	335.57±73.88	278.71±67.13	0.14±0.12	3.05±0.66	1.00±0.00
B组	30	334.52±89.20	334.84±86.34	278.28±80.92	0.12±0.76	3.10±0.64	1.00±0.00
C组	34	333.46±75.74	330.79±68.62	268.56±69.66	0.12±0.08	3.08±0.58	1.00±0.01
F值		0.172	0.153	0.153	0.008	0.040	0.434
P值		0.859	0.860	0.778	0.998	0.994	0.532

下载: 导出CSV

表 3 肠道菌群相对丰度与临床症状的相关性(r值)

肠道菌群	阳性因子	阴性因子	认知因子	兴奋因子	抑郁因子	总分
小杆菌属	-0.180	-0.230	-0.217	-0.153	-0.269^a	-0.123
嗜黏蛋白阿克曼病菌属	0.070	0.209	0.138	0.113	0.076	0.157
考拉杆菌属	0.137	0.030	0.125	0.068	0.123	0.013
瘤胃球菌属	0.141	0.158	0.086	0.121	-0.097	0.208
罗斯氏菌属	0.131	-0.129	-0.058	0.009	0.080	-0.111
巨单胞菌属	0.090	0.226	0.204	0.028	-0.196	0.291^a
埃希氏杆菌属	0.181	-0.145	-0.053	0.068	-0.215	0.091
粪杆菌属	0.062	0.075	-0.020	-0.020	0.136	-0.054
普氏菌属	-0.197	-0.249	-0.247	-0.080	-0.215	-0.120
拟杆菌属	0.282^a	0.082	0.103	0.004	0.082	0.123
注：^aP < 0.05。

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