血浆Aβ1-40及血脂与炎性指标在阿尔茨海默病患者中的表达及意义

李伟英; 林青; 傅攀

doi:10.16766/j.cnki.issn.1674-4152.002233

血浆Aβ1-40及血脂与炎性指标在阿尔茨海默病患者中的表达及意义

doi: 10.16766/j.cnki.issn.1674-4152.002233

台州市第一人民医院神经内科，浙江台州 318000

基金项目:

浙江省医药卫生计划项目 2019RC310

详细信息

通讯作者:
李伟英，E-mail：liweiyingliwei1@163.com

中图分类号: R742.5 R749.16
计量
- 文章访问数: 168
- HTML全文浏览量: 83
- PDF下载量: 4
- 被引次数: 0
出版历程
- 收稿日期: 2020-04-15
- 网络出版日期: 2022-03-02

Expression and significance of plasma Aβ1-40, blood lipid and inflammatory indexes in Alzheimer's disease

Department of Neurology, Taizhou First People's Hospital, Taizhou, Zhejiang 318020, China

摘要

摘要: 目的探讨血浆β淀粉样蛋白1-40(Aβ1-40)、血脂及炎性指标在老年阿尔茨海默病患者中的表达及临床意义。方法选择2017年6月—2019年10月台州市第一人民医院接诊的老年阿尔茨海默病患者93例作为观察组，依据ADAS-Cog表分组：53例轻度(ADAS-Cog评分＜20分)，40例中重度(ADAS-Cog评分≥20分)。另选择同期于本院行健康体检的50例正常老年人作为对照组，通过酶联免疫吸附试验检测血浆Aβ1-40、炎性指标[白细胞介素1β(IL-1β)、白细胞介素6(IL-6)、肿瘤坏死因子α(TNF-α)]表达，并经全自动生化分析仪检测血脂[三酰甘油(TG)、高密度脂蛋白(HDL)、胆固醇(TC)、低密度脂蛋白(LDL)]表达。结果观察组患者血浆Aβ1-40、IL-1β、IL-6、TNF-α表达均比对照组高[(109.37±8.52)pg/mL vs. (93.48±7.23)pg/mL、(49.53±6.09)pg/mL vs. (30.51±7.46)pg/mL、(170.87±12.71)pg/mL vs. (75.15±8.27)pg/mL、(65.93±5.72)pg/mL vs. (36.70±5.24)pg/mL，均P < 0.05]；2组HDL水平对比差异无统计学意义(均P＞0.05)；观察组患者血脂TG、TC、LDL水平均比对照组高(均P＜0.05)；中重度组患者血浆Aβ1-40、IL-1β、IL-6、TNF-α表达水平均比轻度组高(均P＜0.05)；轻度组、中重度组血脂指标TG、HDL、TC、LDL水平对比差异无统计学意义(均P＞0.05)。结论血浆Aβ1-40、血脂及炎性因子表达与老年阿尔茨海默病病情严重程度密切相关，可为临床评估老年阿尔茨海默病病情进展提供参考依据。
- 血浆β淀粉样蛋白1-40 /
- 血脂 /
- 炎性指标 /
- 阿尔茨海默病
Abstract: Objective To investigate the expression and clinical significance of plasma β-amyloid 1-40 (Aβ1-40), blood lipids and inflammatory indexes in elderly patients with Alzheimer's disease. Methods A total of 93 cases of elderly patients with Alzheimer's disease in Taizhou First People's Hospital from June 2017 to October 2019 were selected as the observation group. All patients were grouped according to the ADAS-Cog table: 53 cases were mild (ADAS-Cog score < 20 points), and 40 cases were moderate to severe (ADAS-Cog score ≥ 20 points). In addition, 50 normal elderly people who underwent health examinations in our hospital during the same period were selected as the control group. The expression of plasma Aβ1-40 and inflammatory indicators [interleukin 1β (IL-1β), IL-6, tumour necrosis factor-α (TNF-α)] were detected by enzyme-linked immunosorbent assay, and the expression of blood lipids [triacylglycerol (TG), high-density lipoprotein (HDL), cholesterol (TC), low-density lipoprotein (LDL)] were detected by a fully automatic biochemical analyser. Results The expressions of plasma Aβ1-40, IL-1β, IL-6 and TNF-α were higher in the observation group than in the control group [(109.37±8.52) pg/mL vs. (93.48±7.23) pg/mL, (49.53±6.09) pg/mL vs. (30.51±7.46) pg/mL, (170.87±50.71) pg/mL vs. (75.15±45.27) pg/mL, (65.93±5.72) pg/mL vs. (36.70±5.24) pg/mL, all P < 0.05]. No statistically significant difference was observed in the HDL levels between the two groups of patients (all P>0.05). The blood lipid levels of TG, TC and LDL were higher in the observation group than in the control group (all P < 0.05). The levels of plasma Aβ1-40, IL-1β, IL-6 and TNF-α were higher in the moderate to severe group than in the mild group (all P < 0.05). No significant difference were observed in the levels of serum TG, HDL, TC and LDL in the mild and moderate groups (all P>0.05). Conclusion The expressions of plasma Aβ1-40, blood lipids and inflammatory factors are closely related to the severity of Alzheimer's disease in the elderly, which can provide reference for the clinical evaluation of the progress of Alzheimer's disease in the elderly.
- Plasma Aβ1-40 /
- Blood lipids /
- Inflammatory indexes /
- Alzheimer's disease

HTML全文

表 1 2组研究对象血浆Aβ1-40、血脂及炎性指标表达水平对比(x±s)

组别	例数	Aβ1-40 (pg/mL)	IL-1β (pg/mL)	IL-6 (pg/mL)	TNF-α (pg/mL)	TG (mmol/L)	HDL (mmol/L)	TC (mmol/L)	LDL (mmol/L)
对照组	50	93.48±7.23	30.51±7.46	75.15±8.27	36.70±5.24	1.04±0.67	1.10±0.38	3.95±1.10	2.40±0.44
观察组	93	109.37±8.52	49.53±6.09	170.87±12.71	65.93±5.72	1.76±0.58	1.13±0.39	5.06±0.93	3.22±0.79
t值		24.149	16.437	48.026	29.990	6.700	0.443	6.378	6.788
P值		< 0.001	< 0.001	< 0.001	< 0.001	< 0.001	0.659	< 0.001	< 0.001

下载: 导出CSV

表 2 不同严重程度老年阿尔茨海默病患者血浆Aβ1-40、血脂及炎性指标水平对比(x±s)

组别	例数	Aβ1-40 (pg/mL)	IL-1β (pg/mL)	IL-6 (pg/mL)	TNF-α (pg/mL)	TG (mmol/L)	HDL (mmol/L)	TC (mmol/L)	LDL (mmol/L)
轻度组	53	107.59±6.78	40.54±6.32	152.60±47.59	60.41±5.70	1.72±0.61	1.09±0.42	4.98±1.01	3.20±0.82
中重度组	40	111.15±10.26	58.52±5.86	189.14±53.83	71.45±7.18	1.80±0.55	1.17±0.36	5.14±0.85	3.24±0.76
t值		2.012	14.011	3.464	8.266	0.653	0.966	0.809	0.240
P值		0.047	< 0.001	0.001	< 0.001	0.516	0.337	0.421	0.811

下载: 导出CSV

参考文献(20)

[1]	赵傲楠, 仇颖慧, 闫艺, 等. 炎症相关标记物在遗忘型轻度认知功能障碍和阿尔茨海默病中诊断价值的初步研究[J]. 临床内科杂志, 2018, 35(5): 316-319. doi: 10.3969/j.issn.1001-9057.2018.05.007
[2]	周利. 阿尔茨海默病发病过程Aβ降解酶的变化规律和相互关系以及初步药物研究[D]. 上海: 华东理工大学, 2016.
[3]	郭盼, 李菁媛, 李琳, 等. 老年女性阿尔茨海默病病人血脂及甲状腺激素水平[J]. 实用老年医学, 2019, 33(4): 389-391. doi: 10.3969/j.issn.1003-9198.2019.04.022
[4]	HE B, CHEN W, ZENG J S, et al. MicroRNA-326 decreases tau phosphorylation and neuron apoptosis through inhibition of the JNK signaling pathway by targeting VAV1 in Alzheimer's disease[J]. J Cell Physiol, 2020, 235(1): 480-493. doi: 10.1002/jcp.28988
[5]	邸畅, 李国萍, 陈绍春. β样淀粉蛋白和早老素增强子-2与环磷酸腺苷应答原件结合蛋白的关系[J]. 中国老年学杂志, 2019, 39(22): 5637-5640. doi: 10.3969/j.issn.1005-9202.2019.22.077
[6]	隗永健, 郇玲, 于淑花, 等. Aβ1-40对大鼠海马CA3区神经干细胞凋亡关系的实验研究[J]. 吉林医学, 2017, 38(11): 2006-2007. doi: 10.3969/j.issn.1004-0412.2017.11.002
[7]	敖鹏, 边晓燕, 费洪新, 等. 通天草提取物对Aβ1-40所致老年痴呆大鼠基底核超微结构影响[J]. 中医药学报, 2017, 45(2): 19-22. doi: 10.3969/j.issn.1002-2392.2017.02.005
[8]	马丽, 武海燕, 张力, 等. 老年阿尔茨海默病患者的尿甲醛和血清炎症因子水平及其临床意义[J]. 广西医学, 2017, 39(5): 633-635. https://www.cnki.com.cn/Article/CJFDTOTAL-GYYX201705016.htm
[9]	CHEN L Z, XU S C, WU T, et al. Abnormal platelet amyloid-β precursor protein metabolism in SAMP8 mice: Evidence for peripheral marker in Alzheimer's disease[J]. J Cell Physiol, 2019, 234(12): 23528-23536. doi: 10.1002/jcp.28921
[10]	王海, 邹小冬, 杨钦钦, 等. 乌灵菌粉对APPswe/PS1dE9阿尔兹海默病模型小鼠认知功能、Aβ1-42和p-Tau蛋白的影响[J]. 中华全科医学, 2019, 17(8): 1279-1281, 1395. https://www.cnki.com.cn/Article/CJFDTOTAL-SYQY201908009.htm
[11]	徐珊珊, 金雪红, 吕圣龙, 等. 阿尔兹海默病早期认知功能障碍与代谢指标及炎性因子的相关性研究[J]. 神经损伤与功能重建, 2019, 14(12): 647-649. https://www.cnki.com.cn/Article/CJFDTOTAL-GWKF201912016.htm
[12]	姜大景, 胡婷婷, 向明清, 等. 阿尔兹海默病的发生与常见血管危险因素的相关性研究[J]. 现代中西医结合杂志, 2017, 26(22): 2422-2424, 2428. doi: 10.3969/j.issn.1008-8849.2017.22.009
[13]	王小晗, 张宁宁. 不同程度阿尔茨海默病患者甲状腺激素、血脂水平变化及相关影响因素[J]. 实用临床医药杂志, 2016, 20(5): 32-35. https://www.cnki.com.cn/Article/CJFDTOTAL-XYZL201605010.htm
[14]	张宁, 张冠群, 刘焕. 不同病程的帕金森病患者血清Aβ1-42、YKL-40、BDNF水平变化及其临床意义[J]. 卒中与神经疾病, 2019, 26(6): 737-738, 749. doi: 10.3969/j.issn.1007-0478.2019.06.023
[15]	李俊毅, 党亮君, 商苏杭, 等. 血浆β淀粉样蛋白水平与认知功能衰退的关系[J]. 中华内科杂志, 2019, 58(9): 656-661.
[16]	任秀敏, 于佳楠, 张楠楠, 等. 阿尔兹海默病早期认知功能障碍和高脂血症的相关性分析[J]. 微量元素与健康研究, 2018, 35(4): 9-10. https://www.cnki.com.cn/Article/CJFDTOTAL-WYJK201804006.htm
[17]	LAZZARO S, OGRINC N, LAMONT L, et al. Ion mobility spectrometry combined with multivariate statistical analysis: Revealing the effects of a drug candidate for Alzheimer's disease on Aβ1-40 peptide early assembly[J]. Anal Bioanal Chem, 2019, 411(24): 6353-6363. doi: 10.1007/s00216-019-02030-7
[18]	王天佐, 薛真真, 朱启顺, 等. 阿尔兹海默病发病机理概述及治疗策略展望[J]. 临床医学进展, 2018, 8(1): 85-92.
[19]	贾新, 韩亚军, 许荣, 等. 西北地区中老年人群ApoE基因分布特征及血脂水平与认知功能障碍的关系[J]. 心脏杂志, 2018, 30(1): 13-16, 25. https://www.cnki.com.cn/Article/CJFDTOTAL-XGNZ201801005.htm
[20]	亢涛. 阿尔茨海默病和血管性痴呆患者血清炎症因子和生化指标的检测及临床意义[J]. 检验医学与临床, 2019, 16(20): 2966-2968. doi: 10.3969/j.issn.1672-9455.2019.20.014