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线粒体动力相关蛋白DRP1对肝癌细胞糖代谢的调控作用研究

耿西林 张颖 李浩 张煜 常虎林

耿西林, 张颖, 李浩, 张煜, 常虎林. 线粒体动力相关蛋白DRP1对肝癌细胞糖代谢的调控作用研究[J]. 中华全科医学, 2022, 20(1): 35-38. doi: 10.16766/j.cnki.issn.1674-4152.002270
引用本文: 耿西林, 张颖, 李浩, 张煜, 常虎林. 线粒体动力相关蛋白DRP1对肝癌细胞糖代谢的调控作用研究[J]. 中华全科医学, 2022, 20(1): 35-38. doi: 10.16766/j.cnki.issn.1674-4152.002270
GENG Xi-lin, ZHANG Ying, LI Hao, ZHANG Yu, CHANG Hu-lin. Regulation of mitochondrial dynamic-related protein 1 on glucose metabolism in liver cancer cells[J]. Chinese Journal of General Practice, 2022, 20(1): 35-38. doi: 10.16766/j.cnki.issn.1674-4152.002270
Citation: GENG Xi-lin, ZHANG Ying, LI Hao, ZHANG Yu, CHANG Hu-lin. Regulation of mitochondrial dynamic-related protein 1 on glucose metabolism in liver cancer cells[J]. Chinese Journal of General Practice, 2022, 20(1): 35-38. doi: 10.16766/j.cnki.issn.1674-4152.002270

线粒体动力相关蛋白DRP1对肝癌细胞糖代谢的调控作用研究

doi: 10.16766/j.cnki.issn.1674-4152.002270
基金项目: 

陕西省自然科学基金青年项目 2020JQ-948

详细信息
    通讯作者:

    常虎林,E-mail: hulinchang008@163.com

  • 中图分类号: R735.7  R730.231

Regulation of mitochondrial dynamic-related protein 1 on glucose metabolism in liver cancer cells

  • 摘要:   目的  探讨线粒体动力相关蛋白1(dynamic-related protein 1, DRP1)在肝癌细胞糖代谢重编程中的作用。  方法  (1) siRNA下调肝癌细胞SNU-739中DRP1表达后,检测对肝癌细胞葡萄糖摄取与乳酸产生的影响,以明确DRP1对肝癌细胞糖酵解的调控作用。(2)siRNA下调肝癌细胞SNU-739中DRP1表达后,检测对肝癌细胞氧耗速率与ATP产生的影响,以明确DRP1对肝癌细胞氧化磷酸化的调控作用。(3)siRNA下调肝癌细胞SNU-739中DRP1表达后,利用质谱检测对糖酵解与线粒体三羧酸循环代谢产物的影响。  结果  (1) 下调DRP1可显著抑制肝癌SNU-739细胞的葡萄糖摄取(siCtrl:si-DRP1#1:si-DRP1#2=1.000±0.069:0.417±0.032:0.400±0.040;F=141.400,P<0.001)与乳酸产生(siCtrl:si-DRP1#1:si-DRP1#2=1.000±0.050:0.327±0.040:0.310±0.036;F=256.700,P<0.001)。(2)下调DRP1可激活肝癌SNU-739细胞的氧耗速率(siCtrl:si-DRP1#1:si-DRP1#2=1.000±0.069:1.623±0.081:1.591±0.046;F=81.720,P<0.001)与ATP产生(siCtrl:si-DRP1#1:si-DRP1#2= 1.000±0.062:1.813±0.093:1.850±0.070;F=119.200,P<0.001)。(3)下调DRP1后,肝癌SNU-739细胞中糖酵解中间产物葡萄糖、丙酮酸与乳酸的水平均显著降低(均P<0.001),而三羧酸循环关键产物柠檬酸、延胡索酸与苹果酸的水平均显著升高(均P<0.001)。  结论  线粒体动力相关蛋白DRP1通过激活糖酵解并抑制氧化磷酸化而促进肝癌细胞的糖代谢重编程。

     

  • 图  1  Western blotting检测siRNA对肝癌SNU-739细胞中DRP1表达的下调效率

    注:A为Western blotting曝光图片;B为Western blotting条带灰度扫描结果(DRP1/β-actin);与siCtrl组比较,aP<0.001。

    图  2  下调DRP1对肝癌SNU-739细胞葡萄糖摄取与乳酸产生的影响

    注:A为葡萄糖摄取相对值;B为乳酸产生相对值;与siCtrl组比较,aP<0.001。

    图  3  下调DRP1对肝癌SNU-739细胞氧耗速率与ATP产生的影响

    注:A为细胞氧耗速率;B为ATP含量;与siCtrl组比较,aP<0.001。

    图  4  下调DRP1对肝癌SNU-739细胞中糖酵解与TCA循环代谢中间代谢产物的影响

    注:A为糖酵解相对水平;B为三羧酸循环相对水平;与siCtrl组比较,aP<0.001。

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出版历程
  • 收稿日期:  2020-05-18
  • 网络出版日期:  2022-03-03

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