心脏交感神经对自发性高血压大鼠心脏的影响研究

袁航; 褚婉飞; 徐强

doi:10.16766/j.cnki.issn.1674-4152.002446

心脏交感神经对自发性高血压大鼠心脏的影响研究

doi: 10.16766/j.cnki.issn.1674-4152.002446

袁航^1, ,,
褚婉飞²,
徐强³

1.
宁波市医疗中心李惠利医院电生理科，浙江宁波 315000
2.
宁波市北仑区人民医院心电图室，浙江宁波 315800
3.
浙江省人民医院心内科，浙江杭州 310014

基金项目:

浙江省医药卫生科技计划项目 2016KYA011

详细信息

通讯作者:
袁航，E-mail：65473526@qq.com

中图分类号: R544.1 R-4332
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- 文章访问数: 131
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- PDF下载量: 1
- 被引次数: 0
出版历程
- 收稿日期: 2021-09-14
- 网络出版日期: 2022-09-05

Effect of cardiac sympathetic nerve on the heart of spontaneously hypertensive rats

1.
Department of Electrophysiology, Ningbo Medical Centre Lihuili Hospital, Ningbo, Zhejiang 315000, China

摘要

摘要: 目的探讨心脏交感神经对自发性高血压大鼠心脏的影响。方法选取6~7周龄自发性高血压雄性大鼠30只，按照随机数表法分为观察组与对照组，各15只。观察组大鼠予以交感神经节切除术，对照组大鼠暴露颈中交感神经节。比较2组大鼠心率、血压、左心室舒张、收缩末期内径、左心室射血分数(LVEF)以及心肌胶原组织Ⅰ、心肌胶原组织Ⅲ、结缔组织生长因子(CTGF)、去甲肾上腺素转运蛋白(NET)mRNA表达水平。结果观察组心率明显低于对照组[(374.48±17.46)次/min vs. (403.12±14.56)次/min，t=4.879，P＜0.05]；观察组大鼠左心室舒张末期内径及左心室收缩末期内径明显大于对照组大鼠[(4.97±0.52)mm vs. (4.51±0.45)mm，(3.06±0.23)mm vs. (2.57±0.17)mm，t=2.591、6.635，均P＜0.05]，LVEF明显低于对照组[(71.48±4.46)% vs. (80.12±3.56)%，t=5.864，P＜0.05]；观察组大鼠NET mRNA表达明显高于对照组(1.507±0.054 vs. 1.424±0.046，t=4.532，P < 0.05)，心肌胶原组织Ⅲ、CTGF mRNA表达明显低于对照组(1.245±0.046 vs. 1.359±0.036；1.434±0.095 vs. 1.541±0.012，t=7.559、4.328，均P＜0.05)。结论心脏交感神经可通过调节CTGF、NET水平，抑制自发性高血压大鼠心肌肥厚及心肌纤维化进程。
- 自发性高血压 /
- 心脏交感神经 /
- 心率 /
- 信使RNA表达
Abstract: Objective To investigate the effect of cardiac sympathetic nerve on the heart of spontaneously hypertensive rats. Methods Thirty spontaneously hypertensive male rats aged 6-7 weeks were selected and divided into the observation group and control group according to the random number table method, each with 15 rats. The rats in the observation group were treated with sympathetic ganglion resection, and the sympathetic ganglion in the neck of the rats in the control group was exposed. The heart rate, blood pressure, left ventricular diastole, end-systolic diameter, left ventricular ejection fraction (LVEF) and myocardial collagen tissue Ⅰ, myocardial collagen tissue Ⅲ, connective tissue growth factor and norepinephrine transporter mRNA expression levels were compared between the two groups of rats. Results The heart rate of the observation group was significantly lower than that of the control group [(374.48±17.46) times/min vs. (403.12±14.56) times/min, t=4.879, P < 0.05]; The left ventricular end-diastolic diameter and left ventricular end-systolic diameter of the observation group were significantly lower than those in the control group [(4.97±0.52) mm vs. (4.51±0.45) mm, (3.06±0.23) mm vs. (2.57±0.17) mm, t=2.591, 6.635, all P < 0.05], The LVEF of the observation group was significantly lower than that in the control group [(71.48±4.46)% vs. (80.12±3.56)%, t=5.864, P < 0.05]; The expression of NET mRNA in the observation group were significantly higher than that in the control group (1.507±0.054 vs. 1.424±0.046, t=4.532, P < 0.05), and the expression of myocardial collagen Ⅲ and CTGF mRNA was significantly lower than that in the control group (1.245±0.046 vs. 1.359±0.036, 1.434±0.095 vs. 1.541±0.012, t=7.559, 4.328, P < 0.05). Conclusion Cardiac sympathetic nerve can adjust the CTGF, NET, inhibit myocardial hypertrophy and myocardial fibrosis in spontaneously hypertensive rats.
- Spontaneous hypertension /
- Cardiac sympathetic nerve /
- Heart rate /
- Messenger RNA expression

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表 1 2组大鼠心率及血压比较(x±s)

Table 1. Comparison of heart rate and blood pressure between the two groups(x±s)

组别	例数	收缩压(mm Hg)	舒张压(mm Hg)	心率(次/min)
对照组	15	241.41±17.45	148.57±23.82	403.12±14.56
观察组	15	234.21±12.63	152.06±16.13	374.48±17.46
t值		1.295	0.470	4.879
P值		0.206	0.642	＜0.001
注：1 mm Hg=0.133 kPa。

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表 2 2组大鼠超声心动图检测结果比较(x±s)

Table 2. The results of echocardiography were compared between the two groups(x±s)

组别	例数	左心室舒张末期内径(mm)	左心室收缩末期内径(mm)	LVEF (%)
对照组	15	4.51±0.45	2.57±0.17	80.12±3.56
观察组	15	4.97±0.52	3.06±0.23	71.48±4.46
t值		2.591	6.635	5.864
P值		0.015	＜0.001	＜0.001

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表 3 2组大鼠左心室心肌组织各指标mRNA表达水平比较(x±s)

Table 3. The mRNA expression levels of left ventricular myocardial tissue were compared between the two groups(x±s)

组别	例数	心肌胶原组织Ⅰ	心肌胶原组织Ⅲ	CTGF	NET
对照组	15	1.306±0.032	1.359±0.036	1.541±0.012	1.424±0.046
观察组	15	1.301±0.031	1.245±0.046	1.434±0.095	1.507±0.054
t值		0.435	7.559	4.328	4.532
P值		0.667	＜0.001	＜0.001	＜0.001

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参考文献(26)

[1]	魏明慧, 薛明明. 高血压性心肌肥厚相关信号通路的研究进展[J]. 中国心血管病研究, 2020, 18(5): 460-464. doi: 10.3969/j.issn.1672-5301.2020.05.015 WEI M H, XUE M M. Research progress of related signal pathway for hypertensive cardiac hypertrophy[J]. Chinese Journal of Cardiovascular Research, 2020, 18(5): 460-464. doi: 10.3969/j.issn.1672-5301.2020.05.015
[2]	中国老年学和老年医学学会心血管病分会, 中国高血压联盟. β受体阻滞剂治疗高血压的临床应用建议[J]. 中华心血管病杂志, 2019, 47(6): 443-446. doi: 10.3760/cma.j.issn.0253-3758.2019.06.004 Chinese Society of Gerontology and Geriatrics Cardiovascular Disease Branch, Chinese Hypertension League. Recommendation on the application of β-blockers in the treatment of hypertension[J]. Chinese Journal of Cardiology, 2019, 47(6): 443-446. doi: 10.3760/cma.j.issn.0253-3758.2019.06.004
[3]	刘利勤, 李青, 胡明, 等. 应用Markov模型对3种血管紧张素Ⅱ受体拮抗剂预防高血压患者卒中和心肌梗死的经济学评价[J]. 中国药学杂志, 2019, 54(2): 137-143. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGYX201902010.htm LIU L Q, LI Q, HU M, et al. The economic evaluation of three kinds of angiotensin Ⅱ receptor blockers in stroke and myocardial infarction prevention among hypertension patients using Markov model[J]. Chinese Pharmaceutical Journal, 2019, 54(2): 137-143. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGYX201902010.htm
[4]	吴晓飞. 高血压急症识别与处理[J]. 中华全科医学, 2021, 19(1): 4-5. http://www.zhqkyx.net/article/id/7811fbee-ac5f-4dc8-914f-8c1fc9cbf2de WU X F. Emergency identification and management of hypertension[J]. Chinese general practice, 2021, 19(1): 4-5. http://www.zhqkyx.net/article/id/7811fbee-ac5f-4dc8-914f-8c1fc9cbf2de
[5]	HU L, WANG J, HUANG H, et al. YTHDF1 regulates pulmonary hypertension through translational control of MAGED1[J]. Am J Respir Crit Care Med, 2021, 203(9): 1158-1172. doi: 10.1164/rccm.202009-3419OC
[6]	MARTIN N, MANOHARAN K, DAVIES C, et al. Beta-blockers and inhibitors of the renin-angiotensin aldosterone system for chronic heart failure with preserved ejection fraction[J]. Cochrane Database Syst Rev, 2021, 5(5): CD012721. DOI: 10.1002/14651858.CD012721.pub3.
[7]	常乐, 李强, 廖建文, 等. 高血压患者自发性压力反射敏感性与靶器官损害的关系[J]. 中华高血压杂志, 2018, 26(7): 627-633. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGGZ201807013.htm CHANG L, LI Q, LIAO J W, et al. The relationship between the spontaneous baroreflex sensitivity and target organ damage in hypertensive patients[J]. Chinese Journal of Hypertension, 2018, 26(7): 627-633. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGGZ201807013.htm
[8]	肖冰, 杨秀春, 鲁静朝, 等. ADMA在交感神经损毁自发性高血压大鼠中的作用[J]. 安徽医科大学学报, 2018, 53(3): 382-385. https://www.cnki.com.cn/Article/CJFDTOTAL-YIKE201803011.htm XIAO B, YANG X C, LU J C, et al. Effect of ADMA in sympathectomized spontaneously hypertensive rat[J]. Acta Universitatis Medicinalis Anhui, 2018, 53(3): 382-385. https://www.cnki.com.cn/Article/CJFDTOTAL-YIKE201803011.htm
[9]	郭建强, 李素娟, 贾宇臣. 儿茶酚胺抑素在自发性高血压大鼠交感神经活化中的作用[J]. 中国现代医学杂志, 2019, 29(5): 12-16. doi: 10.3969/j.issn.1005-8982.2019.05.003 GUO J Q, LI S J, JIA Y C. Role of Catestatin in sympathetic activation in spontaneously hypertensive rats[J]. China Journal of Modern Medicine, 2019, 29(5): 12-16. doi: 10.3969/j.issn.1005-8982.2019.05.003
[10]	DELALIO L J, SVED A F, STOCKER S D. Sympathetic nervous system contributions to hypertension: Updates and therapeutic relevance[J]. Can J Cardiol, 2020, 36(5): 712-720.
[11]	丁宇, 李世军. 心脏交感神经对自发性高血压大鼠心肌肥厚与心肌纤维化调节作用[J]. 中华老年心脑血管病杂志, 2018, 20(12): 1298-1301. doi: 10.3969/j.issn.1009-0126.2018.12.017 DING Y, LI S J. Effect of cardiac sympathetic nerve on myocardial hypertrophy and fibrosis in spontaneous hypertension rats[J]. Chinese Journal of Geriatric Heart Brain and Vessel Diseases, 2018, 20(12): 1298-1301. doi: 10.3969/j.issn.1009-0126.2018.12.017
[12]	牛丹丹, 吕本艳. 原发性高血压病患者交感神经和迷走神经张力对静息心率的影响[J]. 新乡医学院学报, 2020, 37(5): 430-432. https://www.cnki.com.cn/Article/CJFDTOTAL-XXYX202005009.htm NIU D D, LYU B Y. Effects of sympathetic nerve and vagal nerve tension on resting heart rate in patients with essential hypertension[J]. Journal of Xinxiang Medical University, 2020, 37(5): 430-432. https://www.cnki.com.cn/Article/CJFDTOTAL-XXYX202005009.htm
[13]	贾娜, 曾学寨, 刘德平, 等. 高血压患者动态脉搏波速度与左心室肥厚和左心功能的关系[J]. 中国心血管病研究, 2020, 18(5): 395-400. doi: 10.3969/j.issn.1672-5301.2020.05.003 JIA N, ZENG X Z, LIU D P, et al. Relationship between ambulatory pulse wave velocity and left ventricular structure and function in patients with primary hypertension[J]. Chinese Journal of Cardiovascular Research, 2020, 18(5): 395-400. doi: 10.3969/j.issn.1672-5301.2020.05.003
[14]	ZELT J G E, DEKEMP R A, ROTSTEIN B H, et al. Nuclear imaging of the cardiac sympathetic nervous system: A disease-specific interpretation in heart failure[J]. JACC Cardiovasc Imaging, 2020, 13(4): 1036-1054. doi: 10.1016/j.jcmg.2019.01.042
[15]	KARMACHARYA P, SINGH S, TIWARI I. Evaluation of sympathetic response in offsprings of hypertensive and normotensive parents[J]. J Nepal Health Res Counc, 2020, 17(4): 528-531. doi: 10.33314/jnhrc.v17i4.2270
[16]	KIUCHI M G, HO J K, NOLDE J M, et al. Sympathetic activation in hypertensive chronic kidney disease: A stimulus for cardiac arrhythmias and sudden cardiac death?[J]. Front Physiol, 2020, 14(10): 1546.
[17]	HERING L, RAHMAN M, HOCH H, et al. α2A-adrenoceptors modulate renal sympathetic neurotransmission and protect against hypertensive kidney disease[J]. J Am Soc Nephrol, 2020, 31(4): 783-798. doi: 10.1681/ASN.2019060599
[18]	李榕, 闫琪, 陶宁, 等. NET基因启动子区DNA甲基化在职业紧张所致高血压中的研究[J]. 职业与健康, 2019, 35(9): 1168-1170, 1175. LI R, YAN Q, TAO Y, et al. Study of methylation of DNA on promoter of NET gene in occupational stress-induced hypertension[J]. Occupation and Health, 2019, 35(9): 1168-1170, 1175.
[19]	谭文鹏, 李文杰, 黄兆琦. 上调miR-133a表达水平对自发性高血压大鼠心肌纤维化的影响[J]. 中国病理生理杂志, 2018, 34(6): 1142-1146. doi: 10.3969/j.issn.1000-4718.2018.06.031 TAN W P, LI W J, HUANG Z Q. Up-regulation of miR-133a expression attenuates myocardial fibrosis in spontaneously hypertensive rats[J]. Chinese Journal of Pathophysiology, 2018, 34(6): 1142-1146. doi: 10.3969/j.issn.1000-4718.2018.06.031
[20]	戴永发, 李健玲, 覃翡, 等. 去肾动脉交感神经对自发性高血压大鼠交感肾上腺系统的影响[J]. 中国现代医学杂志, 2020, 30(17): 1-6. doi: 10.3969/j.issn.1005-8982.2020.17.001 DAI Y F, LI J L, ZAO F, et al. Effects of renal artery denervation on sympathoadrenal system in spontaneously hypertensive rats[J]. China Journal of Modern Medicine, 2020, 30(17): 1-6. doi: 10.3969/j.issn.1005-8982.2020.17.001
[21]	冯宇, 周曼丽, 王健章, 等. 替米沙坦对自发性高血压大鼠左心室肥厚相关蛋白质谱的影响[J]. 中华高血压杂志, 2020, 28(8): 750-756. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGGZ202008018.htm FENG Y, ZHOU M L, WANG J Z, et al. Effects of telmisartan on left ventricular hypertrophy related protein profiles in spontaneously hypertensive rats[J]. Chinese Journal of Hypertension, 2020, 28(8): 750-756. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGGZ202008018.htm
[22]	YOO J K, FU Q. Impact of sex and age on metabolism, sympathetic activity, and hypertension[J]. FASEB J, 2020, 34(9): 11337-11346. doi: 10.1096/fj.202001006RR
[23]	李华妮, 郑连营, 王艳艳, 等. 莱菔子配伍蒺藜对自发性高血压大鼠的降压作用及机制研究[J]. 中国中医基础医学杂志, 2021, 27(5): 756-759, 865. https://www.cnki.com.cn/Article/CJFDTOTAL-ZYJC202105016.htm LI H N, ZHENG L Y, WANG Y Y, et al. Experimental study on anti-hypertensive effects and mechanisms of semen raphani combined with tribuli fructus on spontaneous hypertension rats[J]. Journal of Basic Chinese Medicine, 2021, 27(5): 756-759, 865. https://www.cnki.com.cn/Article/CJFDTOTAL-ZYJC202105016.htm
[24]	ROBLES-VERA I, TORAL M, DUARTE J. Microbiota and hypertension: Role of the sympathetic nervous system and the immune system[J]. Am J Hypertens, 2020, 33(10): 890-901.
[25]	WANG Q, DENG F X, ZHU D W. Superoxide anions modulate the effects of alarin in the paraventricular nucleus on sympathetic activity and blood pressure in spontaneously hypertensive rats[J]. Neuropeptides, 2020, 80: 102021.
[26]	SIGURDARDOTTIR H L, KRANZ G S, RAMI-MARK C, et al. Association of norepinephrine transporter methylation with in vivo NET expression and hyperactivity-impulsivity symptoms in ADHD measured with PET[J]. Mol Psychiatr, 2019, 26(3): 1009-1018.