MiRNA-107在注意缺陷多动障碍儿童外周血中表达的初步研究

蒋茂莹; 王尊晖; 陈东; 黄红宇; 朱萍

doi:10.16766/j.cnki.issn.1674-4152.002458

MiRNA-107在注意缺陷多动障碍儿童外周血中表达的初步研究

doi: 10.16766/j.cnki.issn.1674-4152.002458

蒋茂莹¹,
王尊晖²,
陈东³,
黄红宇^1, ,,
朱萍⁴

1.
杭州市儿童医院儿童保健科，浙江杭州 310014
2.
杭州市儿童医院病案统计室，浙江杭州 310014
3.
杭州市儿童医院检验科，浙江杭州 310014
4.
上海市第六人民医院儿科，上海 201306

基金项目:

浙江省医药卫生科技计划项目 2017KY558

详细信息

通讯作者:
黄红宇，E-mail：598454315@qq.com

中图分类号: R748
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- 被引次数: 0
出版历程
- 收稿日期: 2021-05-10
- 网络出版日期: 2022-09-05

Expression of miRNA-107 in peripheral blood of children with attention deficit hyperactivity disorder

1.
Child Health Care Department of Hangzhou Children's Hospital, Hangzhou, Zhejiang 310014, China

摘要

摘要: 目的探讨miRNA-107、miR-34c-3p在注意缺陷多动障碍(ADHD)儿童中的表达意义。方法选取2018年1月—2019年9月就诊于杭州市儿童医院儿童保健发育行为专科门诊的ADHD儿童共37名，设为ADHD组，74例杭州市儿童医院同期健康体检儿童设为健康对照组。用定量逆转录-聚合酶链反应(RT-PCR)方法检测外周循环血中miRNA-107、miR-34c-3p相对表达量；根据ROC曲线分析miRNA-107在诊断ADHD中的灵敏度及特异度；分析miRNA-107表达量与ADHD核心症状之间的相关性。结果 ADHD儿童外周循环中miRNA-107的相对表达量降低，与健康对照组比较差异有统计学意义(4.06±1.57 vs. 4.62±1.30，t=1.994，P=0.049)，miR-34c-3p相对表达量与健康对照组比较差异无统计学意义；miRNA-107的AUC为0.609，灵敏度为97.3%，特异度为67.6%；相关性分析显示，ADHD女童的miRNA-107表达水平与注意缺陷程度呈负相关关系(r=-0.861, P < 0.05)。结论 MiRNA-107可能是潜在的诊断儿童ADHD的生物标记，具有一定的诊断意义。
- 注意缺陷多动障碍 /
- 微RNA-107 /
- 诊断 /
- 生物标记
Abstract: Objective To measure the expression levels of miRNA-107 and miR-34c-3p in children with attention-deficit hyperactivity disorder (ADHD). Methods From January 2018 to September 2019, 37 children were admitted to the Developmental Behaviour Clinic of Children's Health Care in Hangzhou Children's Hospital and were set as the ADHD group. Seventy-four healthy children in our hospital were set as the healthy control group. The relative expression levels of miRNA-107 and miR-34c-3p in peripheral blood were detected by quantitative reverse transcription-polymerase chain reaction. The sensitivity and specificity of miRNA-107 in diagnosing ADHD were analysed according to the receiver operating characteristic (ROC) curve. The correlation between miRNA-107 expression and ADHD core symptoms was analysed. Results The relative expression of miRNA-107 in peripheral circulation was significantly lower in the ADHD group than in the control group (4.06±1.57 vs. 4.62±1.30, t=1.994, P=0.049), whereas miR-34c-3p showed no significant difference. The AUC of miRNA-107 was 0.609, the sensitivity was 97.3% and the specificity was 67.6%. Correlation analysis showed that the expression level of miRNA-107 in ADHD girls was negatively correlated with the degree of attention deficit (r=-0.861, P < 0.05). Conclusion MiRNA-107 may be a potential biomarker of ADHD and has a certain diagnostic significance.
- Attention deficit hyperactivity disorder /
- MicroRNA-107 /
- Diagnoses /
- Biomarker

HTML全文

图 1 miRNA-107诊断儿童ADHD的ROC曲线

Figure 1. ROC curve of miRNA-107 in the diagnosis of ADHD in children

下载: 全尺寸图片幻灯片

表 1 2组儿童危险因素、身高、体重、年龄情况比较

Table 1. Comparison of risk factors, height, weight and age between the two groups

组别	例数	是否早产[例(%)]		是否顺产[例(%)]		胎龄(x±s, 周)	多胞胎状况[例(%)]
组别	例数	是	否	是	否	胎龄(x±s, 周)	是	否
ADHD组	37	2(5.41)	35(94.59)	30(81.08)	7(18.92)	41.50±2.24	4(10.81)	33(89.19)
对照组	74	6(8.11)	68(91.89)	60(81.08)	14(18.92)	41.60±2.03	8(10.81)	66(89.19)
统计量		0.017^a		0.001^a		0.236^b	0.105^a
P值		0.604		0.995		0.814	0.991

组别	例数	出生情况[例(%)]		身高(x±s, cm)		体重(x±s, kg)	年龄(x±s, 岁)
组别	例数	正常	疾病	身高(x±s, cm)		体重(x±s, kg)	年龄(x±s, 岁)
ADHD组	37	36(97.30)	1(2.70)	123.60±25.40		31.30±5.70	8.13±3.56
对照组	74	72(97.30)	2(2.70)	122.90±20.30		31.60±5.10	8.45±3.77
统计量		0.385^a		0.157^b		0.281^b	0.429^b
P值		0.924		0.875		0.779	0.669
注：^a为χ²值，^b为t值。

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表 2 PCR引物序列

Table 2. PCR primer sequence

引物名称	引物序列
miR-15b	5'-TACTGTAGCAGCACATCAT-3'
miR-15bRT	5'-GTCGTATCCAGTGChGGGTCCGAGGTATTCGCACTGGAT ACGACTGTAAA-3'
hsa-miR-34c-3p	5'-GGTGGAATCACTAACCACACG-3'
hsa-miR-34c-3p-RT	5'-GTGCAGGGTCCGAGGT-3'
miRNA-107	5'-GCCGCAGCAGCATTGTACAGGG-3'
hsa-miRNA-107-RT	5'-GTCGTATGCAGTGCAGGGTCCGAGGTATTCGCACTGCAT ACGACTGATAG-3'
RNU6RT	5'-AAAATATGGAACGCT-3'
RNU6a	5'-AACGCTTCACGAATTTGCGT-3'
RNU6s	5'-CTCGCTTCGGCAGCACA-3'

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表 3 ADHD组及健康对照组儿童miRNA-107、miR-34c-3p表达水平比较(x±s)

Table 3. The expression levels of miRNA-107 and miR- 34C -3p in children with ADHD and healthy controls(x±s)

组别	例数	has-miRNA-107	hsa-miR-34c-3p
ADHD组	37	4.06±1.57	1.76±0.03
健康对照组	74	4.62±1.30	1.77±0.03
t值		1.994	1.656
P值		0.049	0.101

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表 4 不同性别ADHD儿童miRNA-107表达量与SNAP-Ⅳ量表得分的相关性(r值)

Table 4. Correlation between the expression of miRNA-107 and the score of SNAP- Ⅳ in ADHD children of different genders (r value)

项目	注意缺陷	多动冲动
男童miRNA-107表达量	0.245	0.244
女童miRNA-107表达量	-0.861^a	0.505
注：^aP < 0.05。

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