MiRNA-155在反复喘息儿童中的表达及与Th1/Th2细胞平衡的关系研究

沈仁; 杨善浦; 吴月超; 周艳; 张林桃

doi:10.16766/j.cnki.issn.1674-4152.002459

MiRNA-155在反复喘息儿童中的表达及与Th1/Th2细胞平衡的关系研究

doi: 10.16766/j.cnki.issn.1674-4152.002459

玉环市人民医院儿科，浙江玉环 317600

基金项目:

浙江省医药卫生科技计划项目 2019KY795

详细信息

通讯作者:
杨善浦，E-mail：ysp6005@qq.com

中图分类号: R725.6
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出版历程
- 收稿日期: 2021-05-10
- 网络出版日期: 2022-09-05

Expression of miRNA-155 in children with recurrent wheezing and its relationship with Th1/Th2 cell balance

Department of Pediatrics, Yuhuan People's Hospital of Zhejiang Province, Yuhuan, Zhejiang 317600, China

摘要

摘要: 目的分析反复喘息儿童miRNA-155的表达量，探讨miRNA-155与喘息及Th1/Th2细胞平衡的可能关系。方法选取2019年1月—2020年10月在玉环市人民医院住院的反复喘息40例儿童为观察组，同期在该院健康体检儿童40例为对照组。检测2组儿童外周血miRNA-155、IFN-γ及IL-4水平。结果观察组miRNA-155表达量为5.24(3.42, 7.45)，对照组miRNA-155表达量为6.52(4.51, 12.88)，观察组miRNA-155表达量显著低于对照组，2组比较差异有统计学意义(P＜0.05)。观察组IFN-γ/IL-4值为0.73±0.09，对照组IFN-γ/IL-4值为0.82±0.10，观察组IFN-γ/IL-4值显著低于对照组，2组比较差异有统计学意义(P＜0.05)。观察组miRNA-155表达量与喘息次数呈负相关关系(r=-0.557，P＜0.05)。观察组miRNA-155表达量与IFN-γ/IL-4值无相关关系(r=0.223，P＞0.05)。结论 miRNA-155表达量可能与儿童喘息发生呈负相关关系，miRNA-155表达量下降可能会增加儿童喘息发生的风险；IFN-γ与IL-4比值下降可能会增加儿童喘息发生的风险，但未发现miRNA-155表达量与Th1/Th2细胞平衡存在明显相关性。
- 微RNA-155 /
- 喘息 /
- 儿童 /
- 细胞平衡
Abstract: Objective To analyse the expression of miRNA-155 in children with recurrent wheezing and to explore the possible relationship between miRNA-155 and wheezing and the balance of Th1/Th2 cells. Methods Forty cases of children with recurrent wheezing in Yuhuan People's Hospital from January 2019 to October 2020 were selected as observation group, and 40 cases of healthy children in our hospital at the same time were selected as control group. The levels of miRNA-155, IFN-γ and IL-4 in the peripheral blood of the two groups were detected. Results The expression of miRNA-155 in the observation group was 5.24(3.42, 7.45), and that in the control group was 6.52(4.51, 12.88). The expression of miRNA-155 in the observation group was significantly lower than that in the control group (P < 0.05). The IFN-γ/IL-4 value of the observation group was 0.73±0.09, and that of the control group was 0.82±0.10, and the difference was statistically significant (P < 0.05). The expression of miRNA-155 was negatively correlated with the number of wheezing in the observation group (r=-0.557, P < 0.05). No correlation was observed between the expression of miRNA-155 and IFN-γ/IL-4 value in the observation group (r=0.223, P>0.05). Conclusion The expression of miRNA-155 may be negatively correlated with the occurrence of wheezing in children, and the decreased expression of miRNA-155 may increase the risk of wheezing in children. Decreased IFN-γ-to-IL-4 ratio may increase the risk of wheezing in children, but there is no significant correlation between the expression of miRNA-155 and the balance of Th1/Th2 cells.
- MicroRNA-155 /
- Wheezing /
- Children /
- Cell balance

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表 1 2组儿童IFN-γ、IL-4及IFN-γ/IL-4值比较(x±s)

Table 1. The values of IFN-γ, IL-4 and IFN-γ/IL-4 in two groups of children were compared(x±s)

组别	例数	IFN-γ(pg/mL)	IL-4(pg/mL)	IFN-γ/IL-4
观察组	40	125.56±58.84	137.01(126.14, 216.99)	0.73±0.09
对照组	40	119.84±37.67	126.36(106.84, 187.41)	0.82±0.10
统计量		0.518^a	-2.242^b	-3.839^a
P值		0.606	0.025	< 0.001
注：^a为t值，^b为Z值。

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