吲哚美辛对内镜下逆行胆胰管造影术后外周血辅助性T细胞的影响

杨清峰; 王华冠; 韩亮; 雷家才; 黄海涛; 燕善军

doi:10.16766/j.cnki.issn.1674-4152.002500

吲哚美辛对内镜下逆行胆胰管造影术后外周血辅助性T细胞的影响

doi: 10.16766/j.cnki.issn.1674-4152.002500

1.
杭州市第九人民医院消化内科, 浙江杭州 311225
2.
杭州市第一人民医院消化内科, 浙江杭州 310003
3.
蚌埠医学院第一附属医院消化内科, 安徽蚌埠 233004

基金项目:

浙江省医药卫生科技计划项目 2020KY704

杭州市卫生科技计划项目 B20200616

详细信息

通讯作者:
杨清峰, E-mail: yangqingfeng7499@sina.com

中图分类号: R575.7 R576
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出版历程
- 收稿日期: 2021-03-24
- 网络出版日期: 2022-09-21

Effect of indomethacin on helper T cells in peripheral blood after endoscopic retrograde cholangiopancreatography

1.
Department of Gastroenterology, Hangzhou Ninth People ' s Hospital, Hangzhou, Zhejiang 311225, China

摘要

摘要: 目的探讨吲哚美辛栓纳肛对预防内镜下逆行胆胰管造影术(ERCP)后胰腺炎(PEP)可能的细胞学机制。方法选择2017年3-10月在蚌埠医学院第一附属医院消化内科住院并拟行ERCP术的患者40例, 按随机数字表法随机分为观察组和对照组, 每组20例, 分别检测2组患者ERCP术前及术后12 h外周静脉血调节性T细胞(Treg)、辅助性T细胞17型(Th17)及Treg/Th17值。结果对照组: 术前Treg、Th17、Treg/Th17分别为2.94(1.52, 3.71)%、1.42(0.87, 4.47)%、1.57(0.69, 2.88), 术后分别为1.92(1.22, 3.25)%、2.02(1.12, 4.93)%、0.77(0.37, 1.75), 术前术后比较差异均有统计学意义。观察组: 术前Treg、Th17、Treg/Th17分别为1.57(0.97, 2.37)%、1.92(1.27, 3.05)%、0.74(0.39, 2.28);术后分别为1.63(1.03, 1.86)%、2.26(1.36, 3.65)%、0.65(0.38, 1.64), 术前术后比较差异均无统计学意义。2组间除术前Treg差异有统计学意义外(Z=-2.259, P=0.024), 其余指标差异均无统计学意义。结论吲哚美辛栓纳肛预防ERCP术后PEP可能机制是维持Treg、Th17细胞免疫平衡, 使ERCP术后早期的炎症反应得到控制。
- 内镜下逆行性胰胆管造影 /
- 胰腺炎 /
- 调节性T细胞 /
- 辅助性T细胞17 /
- 吲哚美辛
Abstract: Objective To investigate the possible cytological mechanism of indomethacin suppository in the prevention of pancreatitis (PEP) after endoscopic retrograde cholangiopancreatography (ERCP). Methods Forty patients who were hospitalised in the Department of Gastroenterology of the First Affiliated Hospital of Bengbu Medical College from March 2017 to October 2017 and planned to undergo ERCP were randomly divided into the observation group and control group, with 20 cases in each group.The peripheral venous blood Treg, Th17 and Treg/Th17 values of the two groups were measured before and 12 h after ERCP. Results In the control group, the preoperative Treg, Th17 and Treg/Th17 values were 2.94(1.52, 3.71)%, 1.42(0.87, 4.47)% and 1.57(0.69, 2.88), and the postoperative values were 1.92(1.22, 3.25)%, 2.02(1.12, 4.93)% and 0.77(0.37, 1.75), respectively.There were significant differences in the preoperative and postoperative changes.In the observation group, the preoperative Treg, Th17 and Treg/Th17 values were 1.57(0.97, 2.37)%, 1.92(1.27, 3.05)% and 0.74(0.39, 2.28), and the postoperative values were 1.63(1.03, 1.86)%, 2.26(1.36, 3.65)% and 0.65(0.38, 1.64), respectively.There was no significant difference between the preoperative and postoperative changes.There was no significant difference in other indexes between the two groups except for the preoperative Treg difference (Z=-2.259, P=0.024). Conclusion The possible mechanism of indomethacin suppository in preventing PEP after ERCP is to maintain the immune balance of Treg and Th17 cells and control the early inflammatory response after ERCP.
- Endoscopic retrograde cholangiopancreatography /
- Pancreatitis /
- Regulatory T cell /
- Helper T cell 17 /
- Indomethacin

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表 1 2组拟行ERCP术患者术前、术后12 h外周血Th17、Treg及Treg/Th17的比较[M(P₂₅, P₇₅)]

Table 1. Comparison of peripheral blood Th17, Treg and Treg/Th17 before and 12 h after ERCP between the two groups[M(P₂₅, P₇₅)]

组别	例数	Treg(%)		Th17(%)		Treg/Th17
组别	例数	术前	术后12 h	术前	术后12 h	术前	术后12 h
观察组	20	1.57(0.97，2.37)	1.63(1.03，1.86)	1.92(1.27，3.05)	2.26(1.36，3.65)	0.74(0.39，2.28)	0.65(0.38，1.64)
对照组	20	2.94(1.52，3.71)	1.92(1.22，3.25)^a	1.42(0.87，4.47)	2.02(1.12，4.93)^a	1.57(0.69，2.88)	0.77(0.37，1.75)^a
Z值		-2.259	-1.190	-0.514	-0.514	-1.542	-0.352
P值		0.024	0.234	0.607	0.607	0.123	0.725
注：与同组术前比较，^aP＜0.05。

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