基于TCGA数据库分析POSTN在头颈部鳞癌中的表达和临床意义

张曼; 周兰柱; 孙哲; 吴俊; 王文忠

doi:10.16766/j.cnki.issn.1674-4152.002525

基于TCGA数据库分析POSTN在头颈部鳞癌中的表达和临床意义

doi: 10.16766/j.cnki.issn.1674-4152.002525

蚌埠医学院第一附属医院耳鼻喉头颈外科，安徽蚌埠 233004

基金项目:

安徽省高校自然科学研究项目 KJ2020A0568

详细信息

通讯作者:
王文忠, E-mail: 13955259093@163.com

中图分类号: R739.91 R730
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出版历程
- 收稿日期: 2022-01-25
- 网络出版日期: 2022-09-21

Analysis of the expression and clinical significance of POSTN in head and neck squamous carcinoma based on TCGA database

Department of Otorhinolaryngology Head and Neck Surgery, the First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233004, China

摘要

摘要: 目的利用在线数据库和生物信息学方法分析POSTN基因在头颈部鳞状细胞癌中的差异表达对其预后的临床意义，寻找头颈部鳞状细胞癌新的生物标志物。方法使用TCGA公共数据库获取头颈部鳞癌患者的基本信息、POSTN的表达情况；利用Kaplan-Meier Plotter分析不同POSTN表达水平的头颈部鳞癌患者生存曲线；采用TIMER数据库分析POSTN与头颈部鳞癌中各种免疫细胞浸润的相关性；GEPIA数据库分析POSTN表达与免疫细胞标志物表达的相关性；GO和KEGG基因富集分析探索POSTN共表达基因的功能及其作用通路。结果 POSTN在头颈部鳞癌中高表达(P＜0.05)，其表达与头颈部鳞癌患者的预后呈正相关；头颈部鳞癌患者的累积生存率与多种免疫细胞的浸润程度相关；POSTN表达水平与头颈部鳞癌中的巨噬细胞浸润程度呈正相关关系(P=0.024)，并与M2型巨噬细胞标志物呈正相关关系(P＜0.001)，POSTN高表达促进巨噬细胞向M2型极化。富集分析显示POSTN与细胞外基质调节过程相关。结论 POSTN表达与头颈部鳞癌患者预后及免疫浸润相关，并且其可能通过调节巨噬细胞极化而导致患者的不良预后。
- POSTN基因 /
- 头颈部鳞癌 /
- 生存分析 /
- 免疫细胞浸润 /
- 巨噬细胞极化
Abstract: Objective To analyse the clinical significance of POSTN differential expression in the diagnosis and prognosis of head and neck squamous cell carcinoma using online databases and bioinformatics methods and to find new biomarkers for this disease. Methods TCGA public database was used to obtain basic information of head and neck squamous carcinoma patients, the expression of POSTN and the correlation between POSTN and clinicopathological parameters of head and neck squamous carcinoma patients. The Kaplan-Meier Plotter was used to analyse the survival curve of head and neck squamous carcinoma patients with different POSTN expression levels. TIMER analysis was used to determine the correlation between POSTN and various immune cell infiltrations in head and neck squamous carcinoma. GEPIA database analysis was used to determine the correlation between POSTN expression and immune cell marker expression. GO and KEGG gene enrichment analysis were used to explore the function of differentially expressed genes and their pathways of action. Results POSTN was highly expressed in head and neck squamous carcinoma (P < 0.05), and its expression was positively correlated with prognosis of head and neck squamous carcinoma patients, the cumulative survival rate of head and neck squamous carcinoma patients was correlated with the degree of infiltration of multiple immune cells. POSTN expression level was positively correlated with the degree of macrophage infiltration in head and neck squamous carcinoma (P=0.024) and significantly positively correlated with M2 macrophage markers (P < 0.001), with high POSTN expression promoting macrophage polarisation towards M2 type. Enrichment analysis showed that POSTN was associated with extracellular matrix regulatory processes. Conclusion POSTN expression is associated with prognosis and immune infiltration in patients with head and neck squamous carcinoma, and it may contribute to poor patient prognosis by regulating macrophage polarisation.
- POSTN /
- Head and neck squamous carcinoma /
- Survival analysis /
- Immune infiltration /
- Macrophage polarisation

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图 1 POSTN在多种癌症中的表达差异

注：ACC为肾上腺皮质癌；BLCA为膀胱尿路上皮癌；BRCA为乳腺浸润癌；BRCA为乳腺浸润癌；CESC为宫颈鳞癌和腺癌；CHOL为胆管癌；COAD为结肠癌；COAD为结肠癌；DLBC为弥漫性大B细胞淋巴瘤；ESCA为食管癌；GBM为多形成性胶质细胞瘤；HNSC为头颈鳞状细胞癌；KICH为肾嫌色细胞癌；KIRC为肾透明细胞癌；KIRP为肾乳头状细胞癌；LAML为急性髓细胞样白血病；LGG为脑低级别胶质瘤；LIHC为肝细胞肝癌；LUAD为肺腺癌；LUSC为肺鳞癌；MESO为间皮瘤；OV为卵巢浆液性囊腺癌；PAAD为胰腺癌；PCPG为嗜铬细胞瘤和副神经节瘤；PRAD为前列腺癌；READ为直肠腺癌；SARC为肉瘤；SKCM为皮肤黑色素瘤；STAD为胃癌；TGCT为睾丸癌；THCA为甲状腺癌；THYM为胸腺癌；UCEC为子宫内膜癌；UCS为子宫肉瘤；UVM为葡萄膜黑色素瘤。^aP＜0.01，^bP＜0.05。

Figure 1. Expression differences of POSTN in various cancers

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图 2 POSTN在头颈部鳞癌中的表达差异

注：^aP＜0.05。红色为头颈部鳞癌组织，灰色为癌旁正常组织。

Figure 2. Expression differences of POSTN in head and neck squamous cell carcinoma

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图 3 POSTN对预后的影响

Figure 3. Effect of POSTN on prognosis

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表 1 头颈部鳞癌中POSTN表达水平与GEPIA数据库中免疫细胞标志物表达水平的相关性

Table 1. Correlation between the expression level of POSTN in head and neck squamous cell carcinoma and the expression level of immune cell markers in GEPIA database

细胞种类	基因标志物	肿瘤		非肿瘤
细胞种类	基因标志物	r值	P值	r值	P值
单核细胞	CD86	0.280	< 0.001	0.190	0.220
	CD115(CSF1R)	0.140	< 0.001	0.280	0.062
肿瘤相关巨噬细胞	CCL2	0.290	< 0.001	0.200	0.190
	CD68	0.330	< 0.001	0.055	0.730
	IL-10	0.370	< 0.001	0.160	0.300
M1型巨噬细胞	INOS(NOS2)	-0.069	0.120	-0.110	0.470
	IRF5	0.010	0.820	-0.200	0.200
	COX2(PTGS2)	-0.041	0.350	0.032	0.320
M2型巨噬细胞	CD163	0.350	< 0.001	0.680	< 0.001
	MS4A4A	0.370	< 0.001	0.420	0.005

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表 2 POSTN的GO功能富集和KEGG通路富集分析

Table 2. GO functional enrichment and KEGG pathway enrichment analysis of POSTN

类别	名称	P值	数量
BP	细胞外结构组织	＜0.001	55
BP	细胞外基质组织	＜0.001	52
BP	骨化作用	＜0.001	35
BP	胶原蛋白纤维组织	＜0.001	16
BP	肌肉系统形成	＜0.001	31
CC	含胶原蛋白的细胞外基质	＜0.001	62
CC	胶原蛋白三聚体	＜0.001	20
CC	内质网腔体	＜0.001	26
CC	纤维状胶原蛋白三聚体	＜0.001	7
CC	带状胶原蛋白纤维	＜0.001	7
MF	细胞外基质结构成分	＜0.001	39
MF	胶原蛋白结合	＜0.001	16
MF	整合素结合	＜0.001	20
MF	赋予抗拉强度的细胞外基质结构成分	＜0.001	13
MF	糖胺聚糖结合	＜0.001	24
KEGG	ECM-受体的相互作用	＜0.001	16
KEGG	PI3K-Akt信号通路	＜0.001	23
KEGG	蛋白质消化吸收	＜0.001	13
KEGG	局部黏附	＜0.001	16
KEGG	人乳头瘤病毒感染	＜0.001	18
注：BP为生物过程，MF为分子功能，CC为细胞定位。

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