CD8<sup>+</sup> T细胞、NK细胞在子宫颈癌患者外周血中的表达及意义

哈提拉·吐尔逊; 美力班·吐尔逊; 刘倩; 李金秋; 艾力菲热·加帕尔; 阿仙姑·哈斯木

doi:10.16766/j.cnki.issn.1674-4152.002581

CD8⁺ T细胞、NK细胞在子宫颈癌患者外周血中的表达及意义

doi: 10.16766/j.cnki.issn.1674-4152.002581

1.
新疆医科大学基础医学院病理学教研室，新疆地方病分子生物学重点实验室，新疆乌鲁木齐 830054
2.
新疆医科大学基础医学院，新疆地方病分子生物学重点实验室，新疆乌鲁木齐 830054

基金项目:

新疆维吾尔自治区自然科学基金项目 2018D01C151

新疆维吾尔自治区研究生科研创新项目 XJ2020G186

详细信息

通讯作者:
阿仙姑·哈斯木, E-mail: axiangu75@126.com

中图分类号: R737.33R446
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- 文章访问数: 212
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- 被引次数: 0
出版历程
- 收稿日期: 2021-12-21
- 网络出版日期: 2022-09-26

Expression and significance of CD8⁺ T cells and NK cells in peripheral blood of patients with cervical cancer

1.
Department of Pathology, School of Basic Medicine, Xinjiang Medical University, Xinjiang Key Laboratory of Molecular Biology for Endemic Diseases, Urumqi, Xinjiang 830054, China

摘要

摘要: 目的分析子宫颈癌患者的外周血中自然杀伤细胞(NK)与CD8⁺T细胞及免疫卡控点具有IG和ITIM域蛋白的T细胞免疫受体(TIGIT)、程序性死亡受体-1(PD1)、程序性死亡配体-1(PDL1)的表达水平和临床意义。方法将2020年9月—2021年2月新疆医科大学第一附属医院收治的14例子宫颈癌患者与14例健康人群分为宫颈癌组与对照组。采用流式细胞术检测CD8⁺ T细胞与NK细胞在外周血中分布比例及免疫卡控点TIGIT、PD1、PDL1的表达水平。结果宫颈癌患者外周血中CD8⁺ T的比例[(22.66±3.79)%]低于对照组[(28.30±8.61)%，P < 0.01]，而NK细胞在宫颈癌外周血中分布的比例与对照组比较差异无统计学意义(P>0.05)。NK细胞中免疫卡控点PDL1在宫颈癌外周血水平为(5.40±4.44)%，低于对照组的(21.03±16.65)%，差异有统计学意义(P < 0.01)。而TIGIT、PD1在宫颈癌外周血的表达与对照组比较差异均无统计学意义(均P>0.05)。CD8⁺ T细胞上TIGIT、PD1、PDL1在宫颈癌患者外周血中的比例与对照组比较差异无统计学意义(P>0.05)。结论检测NK细胞与CD8⁺ T细胞及免疫卡控点可以监测宫颈癌患者的免疫状态，为临床治疗提供参考。
- 宫颈癌 /
- CD8⁺ T细胞 /
- 自然杀伤细胞 /
- 免疫卡控点
Abstract: Objective To analyze the expression levels and clinical significance of natural killer cells (NK), CD8⁺ T cells and immune checkpoint molecules TIGIT, PD1 and PDL1 in peripheral blood of patients with cervical cancer in Xinjiang. Methods Fourteen cervical cancer patients and fourteen healthy people admitted to the First Affiliated Hospital of Xinjiang Medical University from September 2020 to February 2021 were divided into cervical cancer group and control group. Flow cytometry was used to detect the distribution ratio of CD8⁺ T cells and NK cells in peripheral blood and the expression levels of immune checkpoint molecules, TIGIT, PD1 and PDL1. Results The proportion of CD8⁺ T cells in peripheral blood of cervical cancer patients [(22.66±3.79)%] was lower than that of the control group [(28.30±8.61)%, P < 0.01], however, the proportion of NK cells in peripheral blood of cervical cancer patients has no difference compared with the control group (P>0.05). The level of PDL1 in NK cells from peripheral blood of cervical cancer patients [(5.40±4.44)%] was lower than that of the control group [(21.03±16.65)%], the difference was statistically significant (P < 0.01). However, the expression of TIGIT and PD1 in the peripheral blood of cervical cancer patient were not significantly different from those of the control group (all P>0.05). The proportions of TIGIT, PD1 and PDL1 on CD8⁺ T cells in peripheral blood of cervical cancer patients were not significantly different from those of the control group (P>0.05). Conclusion Detection of NK cells, CD8⁺ T cells and immune checkpoint molecules in peripheral blood of patients with cervical cancer can monitor the immune status of cervical cancer patients, which providing reference for clinical treatment.
- Cervical cancer /
- CD8⁺ T cells /
- Natural killer cells /
- Immune checkpoint

HTML全文

图 1 CD8⁺T细胞及NK细胞在宫颈癌患者及对照组外周血中的分布

注：A示CD8⁺ T细胞在对照组外周血中的比例；B示CD8⁺ T细胞在宫颈癌外周血中的比例；C示NK细胞在对照组外周血中的比例；D示NK细胞在宫颈癌外周血中的比例。

Figure 1. CD8⁺ T cells and NK cells in patients with cervical cancer and the control group in the peripheral blood distribution

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图 2 CD8⁺ T细胞及NK细胞中TIGIT、PD1、PDL1在宫颈癌患者中的分布

注：A示TIGIT在CD8⁺ T细胞的比例；B示PD1在CD8⁺ T细胞的比例；C示PDL1在CD8⁺ T细胞的比例；D示TIGIT在NK细胞的比例；E示PD1在NK细胞的比例；F示PDL1在NK细胞的比例。

Figure 2. TIGIT CD8⁺ T cells and NK cells, PD1, PDL1 distribution in patients with cervical cancer

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表 1 2组外周血中CD8⁺ T细胞上TIGIT、PD1、PDL1的表达情况[M(P₂₅, P₇₅)]

Table 1. Expression of TIGIT, PD1 and PDL1 on CD8⁺ T cells in peripheral blood of the two groups [M(P₂₅, P₇₅)]

组别	例数	TIGIT	PD1	PDL1
对照组	14	7.49(4.09，14.77)	4.88(3.11，7.24)	5.60(3.44，5.46)
宫颈癌组	14	6.45(3.45，7.71)	3.31(2.21，4.34)	5.40(3.99，6.27)
Z值		-0.414	-1.562	-0.092
P值		0.168	0.118	0.927

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表 2 2组外周血中NK细胞上TIGIT、PD1、PDL1的表达情况[M(P₂₅, P₇₅)]

Table 2. Expression of TIGIT, PD1 and PDL1 on NK cells in peripheral blood of two groups[M(P₂₅, P₇₅)]

组别	例数	TIGIT	PD1	PDL1
对照组	14	17.57(14.32，23.29)	5.53(2.85，9.77)	21.03±16.65
宫颈癌组	14	18.47(6.23，22.29)	3.96(2.05，10.58)	5.40±4.44
统计量		-0.414^a	-0.597^a	3.393^b
P值		0.679	0.559	0.007
注：^a为Z值，^b为t值。

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