鸡内金对大鼠肾草酸钙结石的防治作用研究

沙聪威; 陈文政; 曾木达; 周诗光; 陈俊虎

doi:10.16766/j.cnki.issn.1674-4152.002626

鸡内金对大鼠肾草酸钙结石的防治作用研究

doi: 10.16766/j.cnki.issn.1674-4152.002626

广东省生物制品与药物研究所，广东广州 510440

基金项目:

广东省中医药局科研项目 20182011

广东省中医药局科研项目 20182012

详细信息

通讯作者:
陈俊虎, E-mail: qq99jj@163.com

中图分类号: R692.4 R28
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- 被引次数: 0
出版历程
- 收稿日期: 2021-09-02
- 网络出版日期: 2022-11-29

Preventive effects of Galli gigerii endothelium corneum on renal calculus in rats

Guangdong Provincial Institute of Biological Products and Materia Medica, Guangzhou, Guangdong 510440, China

摘要

摘要: 目的通过研究鸡内金对大鼠肾草酸钙结石模型的作用，探讨鸡内金对大鼠肾草酸钙结石的防治作用。方法选用SPF级雄性大鼠40只，按体重排序后采用随机数字表法随机分为正常组、模型组和鸡内金高、中、低剂量组，每组8只。正常组给予正常饮水和饲料，模型组给予造模剂(1%乙二醇饮水+上午0.2 mL/只2%氯化铵灌胃)，鸡内金高剂量组[(5.00 g/kg体重]、中剂量组(2.50 g/kg体重)、低剂量组(1.25 g/kg体重)同时在下午分别按0.2 mL/100 g体重灌胃，连续4周。试验结束后测定各组大鼠24 h排尿量和尿钙(Ca)、尿磷(P)的含量，以及肾组织过氧化氢酶(CAT)、丙二醛(MDA)的含量，分析各组大鼠血清CAT、MDA、Ca、P、肌酐(Cr)、BUN含量，观察肾组织病理学变化。结果与模型组比较，鸡内金高剂量组肾CAT[(6.1±1.3)U/mg vs. (4.1±1.2)U/mg，P＜0.05]、血清CAT[(29.8±1.5)U/mL vs. (26.6±1.3)U/mL，P＜0.01]升高，鸡内金高、中、低剂量组肾MDA[(15.71±0.21)nmol/mg、(16.84±0.18)nmol/mg、(17.29±0.11)nmol/mg vs. (17.59±0.19)nmol/mg，均P＜0.01]含量均降低，鸡内金高剂量组血清Cr[(46.5±3.3)mmol/mL vs. (52.1±2.7)mmol/mL，P＜0.01]降低。与模型组比较，鸡内金各剂量组肾组织病理损伤有所减轻。结论鸡内金能有效预防大鼠肾草酸钙结石，并保护肾功能。
- 鸡内金 /
- 肾结石 /
- 大鼠
Abstract: Objective To study the effect of Galli gigerii endothelium corneum on kidney calcium oxalate stone in rat model, and to explore the preventive and therapeutic effect of Galli gigerii endothelium corneum on kidney calcium oxalate stone in rats. Methods Forty SPF male rats were randomly divided into normal group, model group, high-dose, medium-dose and low-dose groups. The normal group rats were given normal drinking water and feed, and the model group rats were given modeling agent (1% glycol water+0.2 mL / only 2% ammonium chloride to fill the stomach in the morning). The rats in high-dose (5.00 g/kg BW), medium-dose (2.50 g/kg BW), and low-dose (1.25 g/kg BW) GGEC groups fill the stomach with 0.2 mL/100 g BW at the same time in the afternoon for four weeks. The 24 h urine output, urinary calcium (Ca), urinary phosphorus content (P), and renal catalase (CAT), malondialdehyde content (MDA), serum CAT, MDA, Ca, P, creatinine (Cr), BUN contents were analyzed, and renal histopathological changes were observed at the end of the experiment. Results Compared with the model group, renal CAT [(6.1±1.3) U/mg vs. (4.1±1.2) U/mg, P < 0.05] and serum CAT [(29.8±1.5) U/mL vs. (26.6±1.3) U/mL, P < 0.01] significantly increased in the high-dose group, whereas renal MDA significantly decreased in the high-, medium-, and low-dose groups [(15.71±0.21) nmol/mg, (16.84±0.18) nmol/mg, (17.29±0.11) nmol/mg vs. (17.59±0.19) nmol/mg, all P < 0.01], respectively. Serum Cr [(46.5±3.3) mmol/mL vs. (52.1±2.7) mmol/mL, P < 0.01] significantly decreased in the high-dose group. Compared with the model group, the pathological damage of renal tissue in each dose group of GGEC was reduced. Conclusion The GGEC can effectively prevent calcium oxalate calculi and protect renal function in rats.
- Galli gigerii endothelium corneum /
- Renal calculus /
- Rats

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图 1 大鼠肾组织切片(HE染色，×50)

注：A为正常组；B为模型组；C为低剂量组；D为中剂量组；E为高剂量组。

Figure 1. Section of rat kidney tissue (HE, ×50)

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表 1 各组大鼠尿液指标比较(x ±s)

Table 1. Comparison of urine indexes of rats in each group (x ±s)

组别	只数	排尿量(mL)	Ca(mmol/mL)	P(mmol/mL)
正常组	8	10.6±1.2	2.30±0.59	8.81±2.45
模型组	8	8.9±1.6	2.94±0.68	9.94±2.57
高剂量组	8	9.8±1.7	2.45±0.56	9.21±2.29
中剂量组	8	9.4±1.5	2.53±0.60	9.48±2.91
低剂量组	8	9.1±1.4	2.79±0.62	9.55±1.81
F值		1.581	1.452	0.238
P值		0.201	0.238	0.915

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表 2 各组大鼠血清生化指标比较(x ±s)

Table 2. Comparison of serum biochemical indexes of rats in each group (x ±s)

组别	只数	Cr(mmol/mL)	BUN(μmol/mL)	P(mmol/mL)	Ca(mmol/mL)
正常组	8	42.0±2.7	4.99±1.16	2.75±0.35	2.31±0.11
模型组	8	52.1±2.7^a	6.63±1.07	3.73±0.56^a	2.58±0.14^a
高剂量组	8	46.5±3.3^ab	5.37±1.31	3.18±0.48	2.46±0.13
中剂量组	8	49.6±2.5^a	5.70±1.11	3.48±0.18^a	2.48±0.16^a
低剂量组	8	50.9±2.7^a	5.87±1.05	3.67±0.61^a	2.59±0.16^a
F值		17.059	2.310	6.054	5.981
P值		＜0.001	0.077	0.001	0.001
注：与正常组比较，^aP＜0.01；与模型组比较，^bP＜0.01。

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表 3 各组大鼠肾组织和血清过氧化应激指标比较(x ±s)

Table 3. Comparison of renal tissue and serum peroxidation stress index of rats in each group (x ±s)

组别	只数	肾CAT(U/mg)	肾MDA(nmol/mg)	血清CAT(U/mL)	血清MDA(nmol/mL)
正常组	8	10.6±1.2	11.84±0.05	33.9±1.3	3.36±0.66
模型组	8	4.1±1.2^a	17.59±0.19^a	26.6±1.3^a	4.28±0.69
高剂量组	8	6.1±1.3^ab	15.71±0.21^ac	29.8±1.5^ac	3.54±0.51
中剂量组	8	5.2±1.4^a	16.84±0.18^ac	27.8±1.0^a	3.71±0.58
低剂量组	8	4.5±1.0^a	17.29±0.11^ac	26.8±1.2^a	3.89±0.76
F值		30.212	1 745.940	45.271	2.394
P值		＜0.001	＜0.001	＜0.001	0.069
注：与正常组比较，^aP＜0.01；与模型对照组比较，^bP＜0.05，^cP＜0.01。

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参考文献(19)

[1]	唐琪琳, 年莉. 中医药治疗肾结石的现代研究概况[J]. 西部中医药, 2020, 33(3): 146-150. https://www.cnki.com.cn/Article/CJFDTOTAL-GSZY202003042.htm TANG Q L, NIAN L. General situation of modern research on treating kidney stone with traditional Chinese medicine[J]. Western Journal of Traditional Chinese Medicine, 2020, 33(3): 146-150. https://www.cnki.com.cn/Article/CJFDTOTAL-GSZY202003042.htm
[2]	吕茜倩, 毛楠, 樊均明. 中医自拟方治疗泌尿系结石临床理法方药规律研究[J]. 山西中医, 2020, 36(8): 57-59. doi: 10.3969/j.issn.1000-7156.2020.08.029 LYU X Q, MAO N, FAN J M. Study on the clinical theory and method of TCM self-made prescription for treating urinary calculi[J]. Shanxi Journal of Traditional Chinese Medicine, 2020, 36(8): 57-59. doi: 10.3969/j.issn.1000-7156.2020.08.029
[3]	翁湘涛, 王树声, 古炽明, 等. 基于数据挖掘方法总结王树声教授治疗上尿路结石的用药经验[J]. 中国中西医结合外科杂志, 2020, 26(5): 991-996. doi: 10.3969/j.issn.1007-6948.2020.05.037 WENG X T, WANG S S, GU Z M, et al. The study of professor Wang Shu-sheng's experience in the treatment of upper urinary calculi based on data mining[J]. Chinese Journal of Surgery of Integrated Traditional and Western Medicine, 2020, 26(5): 991-996. doi: 10.3969/j.issn.1007-6948.2020.05.037
[4]	赵扬, 谭艳云, 刘映红, 等. 中药"四金"治疗泌尿系结石的研究进展[J]. 中医药导报, 2018, 24(10): 110-112. https://www.cnki.com.cn/Article/CJFDTOTAL-HNZB201810034.htm ZHAO Y, TAN Y Y, LIU Y H, et al. The research progress of treating urinary stones with traditional Chinese medical "four gold"[J]. Guiding Journal of Traditional Chinese Medicine and Pharmacy, 2018, 24(10): 110-112. https://www.cnki.com.cn/Article/CJFDTOTAL-HNZB201810034.htm
[5]	吕双喜, 曾凡雄, 邵魁卿, 等. 基于数据挖掘对上尿路结石的中医用药规律及治疗思路探究[J]. 中国医药导报, 2016, 13(31): 101-104. https://www.cnki.com.cn/Article/CJFDTOTAL-YYCY201631025.htm LYU S X, ZENG F X, SHAO K Q, et al. Exploration on regularity of Chinese herbs application and treatment thought for calculus of upper urinary tract based on data mining[J]. China Medical Herald, 2016, 13(31): 101-104. https://www.cnki.com.cn/Article/CJFDTOTAL-YYCY201631025.htm
[6]	王楠, 顾笑妍, 吴怡, 等. 鸡内金的临床应用及药理作用研究概况[J]. 江苏中医药, 2021, 53(1): 77-81. https://www.cnki.com.cn/Article/CJFDTOTAL-JSZY202101027.htm WANG N, GU X Y, WU Y, et al. Research overview of clinical application and pharmacological action of Jineijin[J]. Jiangsu Journal of Traditional Chinese Medicine, 2021, 53(1): 77-81. https://www.cnki.com.cn/Article/CJFDTOTAL-JSZY202101027.htm
[7]	潘德强, 李红, 孟兆慧. 以鸡内金为例探讨中药选用方法[J]. 光明中医, 2015, 30(7): 1571-1572. doi: 10.3969/j.issn.1003-8914.2015.07.111 PAN D Q L, LI H, MENG Z H. Discussion the selection method of traditional Chinese medicine by taking Rhizome of chicken as an example[J]. Guangming Journal Of Chinese Medicine, 2015, 30(7): 1571-1572. doi: 10.3969/j.issn.1003-8914.2015.07.111
[8]	吴学敏, 顾成娟, 欧文娥, 等. 大叶金钱草、鸡内金、桃仁治疗尿石症经验-仝小林三味小方撷萃[J]. 吉林中医药, 2020, 40(7): 868-870. https://www.cnki.com.cn/Article/CJFDTOTAL-ZYJL202007009.htm WU X M, GU C J, OU W E, et al. Experience in the Treatment of urolithiasis with Lysimachia macrophylla, Inner Membrane of Chicken Gizzard and peach kernel: Three prescription by Professor TONG Xiaolin[J]. Jilin Journal of Chinese Medicine, 2020, 40(7): 868-870. https://www.cnki.com.cn/Article/CJFDTOTAL-ZYJL202007009.htm
[9]	赖海标, 刘朝晖, 吴松, 等. 实验性大鼠肾草酸钙结石造模方法筛选研究[J]. 中国实验方剂学杂志, 2010, 16(14): 135-138. doi: 10.3969/j.issn.1005-9903.2010.14.042 LAI H B, LIU C H, WU S, et al. Comparison of experimental renal calculus of calcium oxalate in rats[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2010, 16(14): 135-138. doi: 10.3969/j.issn.1005-9903.2010.14.042
[10]	冯驰. 金钱草提取物对大鼠肾结石模型作用及抗氧化作用的研究[D]. 南昌: 南昌大学, 2016. FENG C. Study on the effect and antioxidant activity of extracts of Pecuniary herb on renal stone model in rats[D]. Nanchang: Nanchang University, 2016.
[11]	SOHGAURA A, BIGONIYA P. A review on epidemiology and etiology of renal stone[J]. Am J Drug Discov Dev, 2017, 7(2): 54-62. doi: 10.3923/ajdd.2017.54.62
[12]	叶涛, 叶章群. 炎症和氧化应激反应与肾结石形成的研究进展[J]. 中华泌尿外科杂志, 2018, 39(9): 711-713. YE T, YE Z Q. Research progress of inflammation and oxidative stress and kidney stone formation[J]. Chinese Journal of Urology, 2018, 39(9): 711-713.
[13]	ZENG G H, MAI Z L, XIA S J, et al. Prevalence of kidney stones in China: An ultrasonography based cross-sectional study[J]. BJU Int, 2017, 120(1): 109-116.
[14]	徐陈, 韩杰. 上尿路结石微创治疗新进展[J]. 承德医学院学报, 2021, 38(2): 149-152. https://www.cnki.com.cn/Article/CJFDTOTAL-CDYX202102018.htm XU C, HAN J. New progress in minimally invasive treatment of upper urinary calculi[J]. Journal of Chengde Medical University, 2021, 38(2): 149-152. https://www.cnki.com.cn/Article/CJFDTOTAL-CDYX202102018.htm
[15]	蔡莹, 陈生, 徐勇. 肾结石患者血清中LncRNA TapSAKI表达及其与预后的关系[J]. 中华全科医学, 2020, 18(5): 739-742. doi: 10.16766/j.cnki.issn.1674-4152.001343 CAI Y, CHEN S, XU Y. Expression of LncRNA TapSAKI in serum of patients with renal calculi and its relationship with prognosis[J]. Chinese Journal of General Practice, 2020, 18(5): 739-742. doi: 10.16766/j.cnki.issn.1674-4152.001343
[16]	高新梅, 戚微岩, 徐寒梅. 肾结石形成机制及治疗方法研究进展[J]. 药物生物技术, 2019, 26(6): 558-561. https://www.cnki.com.cn/Article/CJFDTOTAL-YWSW201906021.htm GAO S M, QI W Y, XU H M. Research advances in the formation mechanism and treatment method of kidney stones[J]. Pharmaceutical Biotechnology, 2019, 26(6): 558-561. https://www.cnki.com.cn/Article/CJFDTOTAL-YWSW201906021.htm
[17]	靳妍, 刘志, 孙宁, 等. 内皮祖细胞移植对百草枯中毒所致急性肺损伤大鼠炎性因子表达的影响[J]. 中华全科医学, 2021, 19(1): 6-9. doi: 10.16766/j.cnki.issn.1674-4152.001715 JIN Y, LIU Z, SUN N, et al. Effects of endothelial progenitor cell transplantation on the expression of inflammatory factors in a rat model of acute lung injury caused by paraquat poisoning[J]. Chinese Journal of General Practice, 2021, 19(1): 6-9. doi: 10.16766/j.cnki.issn.1674-4152.001715
[18]	尹银华, 胡莹, 宋叶, 等. 尿石汤配方颗粒改善肾草酸钙结石大鼠肾功能效果及其机制[J]. 中国医院药学杂志, 2021, 41(1): 17-23. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGYZ202101005.htm YIN Y H, HU Y, SONG Y, et al. Effect and mechanism of Niaoshi Tang formula granules on improving renal function of rats with calcium oxalate calculi[J]. Chinese Journal of Hospital Pharmacy, 2021, 41(1): 17-23. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGYZ202101005.htm
[19]	杨长军, 秦玉洁, 单永玮, 等. 刺蒺藜对大鼠肾草酸钙结石的防治效果及机制[J]. 国际外科学杂志, 2019, 46(1): 39-43. YANG C J, QIN Y J, SHAN Y W, et al. Control effect and mechanism of Tribulus terrestris in kidney calcium oxalate stone in rats[J]. International Journal of Surgery, 2019, 46(1): 39-43.