宏基因组学第二代测序技术辅助诊断内脏利什曼病1例并文献复习

伍增祥; 安康; 李双庆

doi:10.16766/j.cnki.issn.1674-4152.002665

宏基因组学第二代测序技术辅助诊断内脏利什曼病1例并文献复习

doi: 10.16766/j.cnki.issn.1674-4152.002665

四川大学华西医院全科医学中心，四川成都 610041

详细信息

通讯作者:
李双庆, E-mail: 1259594471@qq.com

中图分类号: R531
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- 文章访问数: 147
- HTML全文浏览量: 98
- PDF下载量: 17
- 被引次数: 0
出版历程
- 收稿日期: 2022-02-15
- 网络出版日期: 2022-11-29

Metagenomics next generation sequencing assisted diagnosis of visceral leishmaniasis: A case report and literature review

General Practice Center, West China Hospital, Sichuan University, Chengdu, 610041, China

摘要

摘要: 宏基因组第二代测序技术(metagenomics next generation sequencing, mNGS)具有快速检测广范围病原体的优点。本文报告1例内脏利什曼病(visceral leishmaniasis, VL)。患者为47岁男性，以反复发热起病，先后于几家医院就诊，未明确诊断，予抗细菌治疗及对症治疗，病情无缓解。后经mNGS查见利什曼原虫属(Leishmania)序列，进一步行rK39免疫层析试条检测(rK39 test)，结果为阳性，最终诊断内脏利什曼病。提示mNGS有望应用于诊断内脏利什曼病，其在诊断感染性疾病方面具有重要的参考价值。
- 内脏利什曼病 /
- 宏基因组第二代测序 /
- 感染性疾病 /
- 诊断
Abstract: Metagenomics next generation sequencing (mNGS) has the advantage of rapid detection of a wide range of pathogens. A case of visceral leishmaniasis (VL) was reported. The patient, a 47-year-old male, started with recurrent fever and went to several hospitals without definite diagnosis. He was treated with antibacterial agents and symptomatic treatment, but his condition was not relieved. After that, the Leishmania sequences were found by mNGS, and rK39 immunochromatographic rapid detection (rK39 test) was further performed. The result was positive, and the final diagnosis was visceral leishmaniasis. It is suggested that mNGS is expected to be used in the diagnosis of visceral leishmaniasis, and it has important reference value in the diagnosis of infectious diseases.
- Visceral leishmaniasis /
- Metagenomics next generation sequencing /
- Infectious diseases /
- Diagnosis

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参考文献(18)

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