硬脂酸对血管平滑肌细胞L-型钙通道功能的影响

江传月; 陶亚芬; 吴月; 汪蔷华; 王学故; 丁彪; 李祥

doi:10.16766/j.cnki.issn.1674-4152.002891

硬脂酸对血管平滑肌细胞L-型钙通道功能的影响

doi: 10.16766/j.cnki.issn.1674-4152.002891

1.
蚌埠医学院第一附属医院生殖医学中心，安徽蚌埠 233004
2.
蚌埠医学院第一附属医院感染科实验室，安徽蚌埠 233004
3.
蚌埠医学院第一附属医院分子诊断中心，安徽蚌埠 233004

基金项目:

安徽省自然科学基金青年项目 2008085QH407

详细信息

通讯作者:
李祥，E-mail：li19850115@163.com

中图分类号: R544.1 R589.2
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- 被引次数: 0
出版历程
- 收稿日期: 2022-07-12
- 网络出版日期: 2023-04-19

Effects of stearic acid on the functions of L-type calcium channel in vascular smooth muscle cells

1.
Reproductive Medicine Center, the First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233004, China

摘要

摘要: 目的高脂影响心血管健康，但机制不明。本研究旨在探讨高脂诱导的硬脂酸对血管平滑肌细胞(VSMCs)L-型钙通道(LTCCs)功能的影响。方法将12只4周龄SD大鼠编号后采用抽签方法进行随机分组：对照组(6只)和高脂组(6只)，分别予正常和高脂饲料喂养12周。采用游离脂肪酸试剂盒和股动脉插管分别检测大鼠血游离脂肪酸和血压；利用膜片钳技术检测大鼠肠系膜VSMCs钙电流。结果与对照组比较，高脂饮食可增加大鼠血游离脂肪酸[(0.667±0.027)mmol/L vs. (0.468±0.012)mmol/L, t=6.841, P < 0.001]、收缩压[(136.50±2.19)mmHg vs. (121.20±3.78)mmHg, t=3.507, P=0.006]及舒张压[(87.10±2.42)mmHg vs. (73.70±2.74)mmHg, t=3.660, P=0.004]。与对照组比较，高脂组大鼠肠系膜动脉VSMCs膜上的钙电流显著增加(膜电位在10 mV时pA/pF的比值，对照组为-16.86±2.58, 高脂组为-23.80±2.15, t=4.449, P < 0.001)。硬脂酸可显著增加VSMCs钙通道电流膜电位(10 mV的pA/pF，硬脂酸0 μmol/L: -12.93±2.58, 硬脂酸30 μmol/L: -18.10±2.15, t=4.097, P=0.001)，但不影响其失活和激活。结论高脂饮食诱导产生的硬脂酸可影响肠系膜血管VSMCs膜上L-型钙通道功能。
- 高脂饮食 /
- 硬脂酸 /
- L-型钙通道 /
- 高血压
Abstract: Objective High-fat diet has adverse effects on cardiovascular health, but the mechanism remains unclear. This study aims to investigate the effect of stearic acid induced by high-fat diet on the function of L-type calcium channel in vascular smooth muscle cells(VSMCs). Methods Four week-old SD rats were randomly divided into control group (6 rats) and high-fat group (6 rats) using a lottery method, and fed with normal standard diet and high-fat diet (containing 45% fat) for 12 weeks, respectively. The commercial kits and rat femoral artery cannulation techniques were used to detect blood free fatty acids and rat blood pressures respectively. Calcium currents in rat mesenteric VSMCs was detected by patch clamp technique. Results Compared with control group, the free fatty acids [(0.667±0.027) mmol/L vs. (0.468±0.012) mmol/L, t=6.841, P < 0.001], systolic [(136.50±2.19) mmHg vs. (121.20±3.78) mmHg, t=3.507, P=0.006] and diastolic [(87.10±2.42) mmHg vs. (73.70±2.74) mmHg, t=3.660, P=0.004)] blood pressure was significantly increased in rats with high-fat diet. Patch-clamp results showed that the calcium channel currents were significantly increased in VSMCs of mesenteric artery of the rats with high-fat diet (the ratio of pA/pF at a membrane potential of 10 mV was -16.86±2.58 in control group and -23.80±2.15 in high-fat group, t=4.449, P < 0.001). In addition, stearic acid could significantly increase calcium channel currents (the pA/pF at 10 mV: -12.93±2.58 in 0 μmol/L stearic acid group; -18.10±2.15 in 30 μmol/L stearic acid group; t=4.097, P=0.001) while not affect the calcium channel inactivation and activation. Conclusion Stearic acid induced by high-fat diet can affect the function of L-type calcium channel on VSMCs membrane of mesenteric vessels.
- High-fat diet /
- Stearic acid /
- L-type calcium channel /
- Hypertension

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图 1 饱和脂肪酸对肠系膜血管平滑肌细胞钙通道功能的影响

注：A为钙电流代表图；B为钙电流I-V曲线，与硬脂酸0 μmol/L组比较，^aP < 0.01，^bP < 0.05；C为尼非地平给药前后电流的变化；D为硬脂酸对钙通道激活的影响(激活曲线)；E为硬脂酸对钙通道失活的影响(失活曲线)。

Figure 1. Effect of saturated fatty acids on calcium channel function in mesenteric vascular smooth muscle cells

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